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P45161

- DACB_HAEIN

UniProt

P45161 - DACB_HAEIN

Protein

D-alanyl-D-alanine carboxypeptidase DacB

Gene

dacB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction.By similarity

    Catalytic activityi

    Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei69 – 691Acyl-ester intermediateBy similarity
    Active sitei72 – 721Proton acceptorBy similarity
    Active sitei310 – 3101By similarity
    Binding sitei420 – 4201SubstrateBy similarity

    GO - Molecular functioni

    1. serine-type D-Ala-D-Ala carboxypeptidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. cell cycle Source: UniProtKB-KW
    2. cell division Source: UniProtKB-KW
    3. peptidoglycan biosynthetic process Source: UniProtKB-UniPathway
    4. regulation of cell shape Source: UniProtKB-KW
    5. response to antibiotic Source: UniProtKB-KW

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Antibiotic resistance, Cell cycle, Cell division, Cell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis

    Enzyme and pathway databases

    UniPathwayiUPA00219.

    Protein family/group databases

    MEROPSiS13.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    D-alanyl-D-alanine carboxypeptidase DacB (EC:3.4.16.4)
    Short name:
    DD-carboxypeptidase
    Short name:
    DD-peptidase
    Alternative name(s):
    D-alanyl-D-alanine endopeptidase (EC:3.4.21.-)
    Short name:
    DD-endopeptidase
    Penicillin-binding protein 4
    Short name:
    PBP-4
    Gene namesi
    Name:dacB
    Ordered Locus Names:HI_1330
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Periplasm Curated

    GO - Cellular componenti

    1. periplasmic space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Periplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2626Sequence AnalysisAdd
    BLAST
    Chaini27 – 479453D-alanyl-D-alanine carboxypeptidase DacBPRO_0000027242Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI1330.

    Structurei

    Secondary structure

    1
    479
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi30 – 345
    Beta strandi42 – 498
    Turni50 – 534
    Beta strandi54 – 607
    Helixi68 – 714
    Helixi72 – 8211
    Beta strandi90 – 978
    Beta strandi108 – 1114
    Helixi120 – 13213
    Beta strandi142 – 1454
    Helixi160 – 1623
    Helixi166 – 1683
    Helixi177 – 1793
    Beta strandi180 – 1867
    Beta strandi196 – 1983
    Beta strandi206 – 2083
    Beta strandi212 – 2143
    Turni216 – 2183
    Helixi219 – 2213
    Beta strandi224 – 2296
    Turni230 – 2323
    Beta strandi233 – 2419
    Beta strandi247 – 2526
    Helixi256 – 27015
    Beta strandi289 – 2957
    Helixi299 – 30911
    Helixi312 – 32716
    Helixi333 – 34614
    Beta strandi359 – 3613
    Helixi370 – 38213
    Helixi384 – 3874
    Helixi390 – 3923
    Turni396 – 3983
    Helixi400 – 4023
    Helixi406 – 4083
    Turni411 – 4166
    Beta strandi418 – 4247
    Beta strandi427 – 4359
    Beta strandi441 – 45010
    Helixi463 – 47816

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    3A3DX-ray1.60A/B28-479[»]
    3A3EX-ray2.40A/B28-479[»]
    3A3FX-ray2.10A/B28-479[»]
    3A3IX-ray2.00A/B28-479[»]
    ProteinModelPortaliP45161.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP45161.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S13 family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiCOG2027.
    KOiK07259.
    OMAiFRTIGHE.
    OrthoDBiEOG6NGVQ4.
    PhylomeDBiP45161.

    Family and domain databases

    Gene3Di3.40.710.10. 1 hit.
    InterProiIPR012338. Beta-lactam/transpept-like.
    IPR000667. Peptidase_S13.
    [Graphical view]
    PfamiPF02113. Peptidase_S13. 1 hit.
    [Graphical view]
    PRINTSiPR00922. DADACBPTASE3.
    SUPFAMiSSF56601. SSF56601. 1 hit.
    TIGRFAMsiTIGR00666. PBP4. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P45161-1 [UniParc]FASTAAdd to Basket

    « Hide

    MKKLSSISTA LGSFLLSVSF SLPTFANINV SDLTQKLPEG SNVGFIAKNI    50
    NQNQIIADYN GSTFMLSAST QKVFTAVAAK LALDDQFQFE TALLSNGKIQ 100
    NGNLDGNLIV RFTGDPDLTR GQLYSLLAEL KKQGIKKING DLVLDTSVFS 150
    SHDRGLGWIW NDLTMCFNSP PAAANIDNNC FYAELDANKN PGEIVKINVP 200
    AQFPIQVFGQ VYVADSNEAP YCQLDVVVHD NNRYQVKGCL ARQYKPFGLS 250
    FAVQNTDAYA AEIIQRQLRQ LGIEFNGKVL LPQKPQQGQL LAKHLSKPLP 300
    DLLKKMMKKS DNQIADSLFR AVAFNYYKRP ASFQLGTLAV KSILQKQGIR 350
    FGNSILADGS GLSRHNLVAP KTMLSVLEYI AKNEDKLHLM ETFPIAGVDG 400
    TISGRGGLIS PPLVKNVIAK TGSLKGVYNL AGFMTNARGE KVAFVQFING 450
    YSTGDLESKT KRAPLVQFER NLYNELYKY 479
    Length:479
    Mass (Da):52,686
    Last modified:November 1, 1995 - v1
    Checksum:i632868C61206CB48
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22975.1.
    PIRiA64117.
    RefSeqiNP_439482.1. NC_000907.1.
    WP_005694445.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22975; AAC22975; HI_1330.
    GeneIDi950252.
    KEGGihin:HI1330.
    PATRICi20191345. VBIHaeInf48452_1383.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22975.1 .
    PIRi A64117.
    RefSeqi NP_439482.1. NC_000907.1.
    WP_005694445.1. NC_000907.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    3A3D X-ray 1.60 A/B 28-479 [» ]
    3A3E X-ray 2.40 A/B 28-479 [» ]
    3A3F X-ray 2.10 A/B 28-479 [» ]
    3A3I X-ray 2.00 A/B 28-479 [» ]
    ProteinModelPortali P45161.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI1330.

    Protein family/group databases

    MEROPSi S13.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22975 ; AAC22975 ; HI_1330 .
    GeneIDi 950252.
    KEGGi hin:HI1330.
    PATRICi 20191345. VBIHaeInf48452_1383.

    Phylogenomic databases

    eggNOGi COG2027.
    KOi K07259.
    OMAi FRTIGHE.
    OrthoDBi EOG6NGVQ4.
    PhylomeDBi P45161.

    Enzyme and pathway databases

    UniPathwayi UPA00219 .

    Miscellaneous databases

    EvolutionaryTracei P45161.

    Family and domain databases

    Gene3Di 3.40.710.10. 1 hit.
    InterProi IPR012338. Beta-lactam/transpept-like.
    IPR000667. Peptidase_S13.
    [Graphical view ]
    Pfami PF02113. Peptidase_S13. 1 hit.
    [Graphical view ]
    PRINTSi PR00922. DADACBPTASE3.
    SUPFAMi SSF56601. SSF56601. 1 hit.
    TIGRFAMsi TIGR00666. PBP4. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiDACB_HAEIN
    AccessioniPrimary (citable) accession number: P45161
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. Peptidase families
      Classification of peptidase families and list of entries
    5. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3