Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P45161 (DACB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 102. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (5) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanyl-D-alanine carboxypeptidase DacB

Short name=DD-carboxypeptidase
Short name=DD-peptidase
EC=3.4.16.4
Alternative name(s):
D-alanyl-D-alanine endopeptidase
Short name=DD-endopeptidase
EC=3.4.21.-
Penicillin-binding protein 4
Short name=PBP-4
Gene names
Name:dacB
Ordered Locus Names:HI_1330
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length479 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Not involved in transpeptidation but exclusively catalyzes a DD-carboxypeptidase and DD-endopeptidase reaction By similarity.

Catalytic activity

Preferential cleavage: (Ac)(2)-L-Lys-D-Ala-|-D-Ala. Also transpeptidation of peptidyl-alanyl moieties that are N-acyl substituents of D-alanine.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis.

Subcellular location

Periplasm Potential.

Sequence similarities

Belongs to the peptidase S13 family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 479453D-alanyl-D-alanine carboxypeptidase DacB
PRO_0000027242

Sites

Active site691Acyl-ester intermediate By similarity
Active site721Proton acceptor By similarity
Active site3101 By similarity
Binding site4201Substrate By similarity

Secondary structure

.......................................................................... 479
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P45161 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 632868C61206CB48

FASTA47952,686
        10         20         30         40         50         60 
MKKLSSISTA LGSFLLSVSF SLPTFANINV SDLTQKLPEG SNVGFIAKNI NQNQIIADYN 

        70         80         90        100        110        120 
GSTFMLSAST QKVFTAVAAK LALDDQFQFE TALLSNGKIQ NGNLDGNLIV RFTGDPDLTR 

       130        140        150        160        170        180 
GQLYSLLAEL KKQGIKKING DLVLDTSVFS SHDRGLGWIW NDLTMCFNSP PAAANIDNNC 

       190        200        210        220        230        240 
FYAELDANKN PGEIVKINVP AQFPIQVFGQ VYVADSNEAP YCQLDVVVHD NNRYQVKGCL 

       250        260        270        280        290        300 
ARQYKPFGLS FAVQNTDAYA AEIIQRQLRQ LGIEFNGKVL LPQKPQQGQL LAKHLSKPLP 

       310        320        330        340        350        360 
DLLKKMMKKS DNQIADSLFR AVAFNYYKRP ASFQLGTLAV KSILQKQGIR FGNSILADGS 

       370        380        390        400        410        420 
GLSRHNLVAP KTMLSVLEYI AKNEDKLHLM ETFPIAGVDG TISGRGGLIS PPLVKNVIAK 

       430        440        450        460        470 
TGSLKGVYNL AGFMTNARGE KVAFVQFING YSTGDLESKT KRAPLVQFER NLYNELYKY 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22975.1.
PIRA64117.
RefSeqNP_439482.1. NC_000907.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
3A3DX-ray1.60A/B28-479[»]
3A3EX-ray2.40A/B28-479[»]
3A3FX-ray2.10A/B28-479[»]
3A3IX-ray2.00A/B28-479[»]
ProteinModelPortalP45161.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI1330.

Protein family/group databases

MEROPSS13.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22975; AAC22975; HI_1330.
GeneID950252.
KEGGhin:HI1330.
PATRIC20191345. VBIHaeInf48452_1383.

Phylogenomic databases

eggNOGCOG2027.
KOK07259.
OMAFRTIGHE.
OrthoDBEOG6NGVQ4.
PhylomeDBP45161.

Enzyme and pathway databases

UniPathwayUPA00219.

Family and domain databases

Gene3D3.40.710.10. 1 hit.
InterProIPR012338. Beta-lactam/transpept-like.
IPR000667. Peptidase_S13.
[Graphical view]
PfamPF02113. Peptidase_S13. 1 hit.
[Graphical view]
PRINTSPR00922. DADACBPTASE3.
SUPFAMSSF56601. SSF56601. 1 hit.
TIGRFAMsTIGR00666. PBP4. 1 hit.
ProtoNetSearch...

Other

EvolutionaryTraceP45161.

Entry information

Entry nameDACB_HAEIN
AccessionPrimary (citable) accession number: P45161
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

PATHWAY comments

Index of metabolic and biosynthesis pathways

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names