P45159 (FABA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase
EC=4.2.1.59

Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
Beta-hydroxydecanoyl thioester dehydrase
Trans-2-decenoyl-[acyl-carrier-protein] isomerase
EC=5.3.3.14

Gene names

Name:	fabA
Ordered Locus Names:	HI_1325

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	177 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length By similarity. HAMAP-Rule MF_00405
Catalytic activity	A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O. HAMAP-Rule MF_00405 (3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O. HAMAP-Rule MF_00405 Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein]. HAMAP-Rule MF_00405
Pathway	Lipid metabolism; fatty acid biosynthesis. HAMAP-Rule MF_00405
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the thioester dehydratase family. FabA subfamily.

Ontologies

Keywords
Biological process	Fatty acid biosynthesis Fatty acid metabolism Lipid biosynthesis Lipid metabolism
Cellular component	Cytoplasm
Molecular function	Isomerase Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	fatty acid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity Inferred from electronic annotation. Source: HAMAP 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Inferred from electronic annotation. Source: EC trans-2-decenoyl-acyl-carrier-protein isomerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 177	177	3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase HAMAP-Rule MF_00405		PRO_0000091599
Sites
	Active site	76	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45159 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 17E272B39A11552D	FASTA	177	19,465
	10 20 30 40 50 60 MQNACTLNKK SSYSYDDLLA SGRGELFGKE GPQLPAPTML MMDRIIEMNE ETGAFGKGYI 70 80 90 100 110 120 EAELDIKPEL PFFGCHFIGD PVMPGCLGLD AMWQLVGFYL GWIGGKGKGR ALGVGEVKFT 130 140 150 160 170 GQILPTAKKV VYRIHMKRVI NRKLVMGMAD GEVEVDGRVI YTATDLKVGL FQDTSTF

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22972.1.
PIR	G64116.
RefSeq	NP_439476.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P45159.
SMR	P45159. Positions 7-177.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22972; AAC22972; HI_1325.
GeneID	950257.
KEGG	hin:HI1325.
PATRIC	20191333. VBIHaeInf48452_1377.
Phylogenomic databases
eggNOG	COG0764.
KO	K01716.
OMA	KGRAIST.
ProtClustDB	PRK05174.
Enzyme and pathway databases
UniPathway	UPA00094.
Family and domain databases
HAMAP	MF_00405. FabA.
InterPro	IPR010083. FabA. IPR013114. FabA_FabZ. [Graphical view]
Pfam	PF07977. FabA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01749. fabA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

FABA_HAEIN

Accession

Primary (citable) accession number: P45159

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 94 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program