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Protein

3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase

Gene

fabA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Necessary for the introduction of cis unsaturation into fatty acids. Catalyzes the dehydration of (3R)-3-hydroxydecanoyl-ACP to E-(2)-decenoyl-ACP and then its isomerization to Z-(3)-decenoyl-ACP. Can catalyze the dehydratase reaction for beta-hydroxyacyl-ACPs with saturated chain lengths up to 16:0, being most active on intermediate chain length (By similarity).By similarity

Catalytic activityi

A (3R)-3-hydroxyacyl-[acyl-carrier protein] = a trans-2-enoyl-[acyl-carrier protein] + H2O.
(3R)-3-hydroxydecanoyl-[acyl-carrier-protein] = trans-dec-2-enoyl-[acyl-carrier-protein] + H2O.
Trans-dec-2-enoyl-[acyl-carrier-protein] = cis-dec-3-enoyl-[acyl-carrier-protein].

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei76 – 761By similarity

GO - Molecular functioni

  1. 3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-HAMAP
  2. 3-hydroxyoctanoyl-[acyl-carrier-protein] dehydratase activity Source: UniProtKB-EC
  3. trans-2-decenoyl-acyl-carrier-protein isomerase activity Source: UniProtKB-HAMAP

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Isomerase, Lyase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00094.

Names & Taxonomyi

Protein namesi
Recommended name:
3-hydroxydecanoyl-[acyl-carrier-protein] dehydratase (EC:4.2.1.59)
Alternative name(s):
3-hydroxyacyl-[acyl-carrier-protein] dehydratase FabA
Beta-hydroxydecanoyl thioester dehydrase
Trans-2-decenoyl-[acyl-carrier-protein] isomerase (EC:5.3.3.14)
Gene namesi
Name:fabA
Ordered Locus Names:HI_1325
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cytoplasm By similarity

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 1771773-hydroxydecanoyl-[acyl-carrier-protein] dehydratasePRO_0000091599Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI1325.

Structurei

3D structure databases

ProteinModelPortaliP45159.
SMRiP45159. Positions 7-177.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0764.
KOiK01716.
OMAiFFDCHFK.
OrthoDBiEOG6VB6SH.
PhylomeDBiP45159.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.

Sequencei

Sequence statusi: Complete.

P45159-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQNACTLNKK SSYSYDDLLA SGRGELFGKE GPQLPAPTML MMDRIIEMNE
60 70 80 90 100
ETGAFGKGYI EAELDIKPEL PFFGCHFIGD PVMPGCLGLD AMWQLVGFYL
110 120 130 140 150
GWIGGKGKGR ALGVGEVKFT GQILPTAKKV VYRIHMKRVI NRKLVMGMAD
160 170
GEVEVDGRVI YTATDLKVGL FQDTSTF
Length:177
Mass (Da):19,465
Last modified:November 1, 1995 - v1
Checksum:i17E272B39A11552D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22972.1.
PIRiG64116.
RefSeqiNP_439476.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22972; AAC22972; HI_1325.
GeneIDi950257.
KEGGihin:HI1325.
PATRICi20191333. VBIHaeInf48452_1377.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22972.1.
PIRiG64116.
RefSeqiNP_439476.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP45159.
SMRiP45159. Positions 7-177.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1325.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22972; AAC22972; HI_1325.
GeneIDi950257.
KEGGihin:HI1325.
PATRICi20191333. VBIHaeInf48452_1377.

Phylogenomic databases

eggNOGiCOG0764.
KOiK01716.
OMAiFFDCHFK.
OrthoDBiEOG6VB6SH.
PhylomeDBiP45159.

Enzyme and pathway databases

UniPathwayiUPA00094.

Family and domain databases

Gene3Di3.10.129.10. 1 hit.
HAMAPiMF_00405. FabA.
InterProiIPR010083. FabA.
IPR013114. FabA_FabZ.
IPR029069. HotDog_dom.
[Graphical view]
PfamiPF07977. FabA. 1 hit.
[Graphical view]
SUPFAMiSSF54637. SSF54637. 1 hit.
TIGRFAMsiTIGR01749. fabA. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiFABA_HAEIN
AccessioniPrimary (citable) accession number: P45159
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 103 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.