P45148 (CAN_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Carbonic anhydrase 2
EC=4.2.1.1

Alternative name(s):
Carbonate dehydratase 2

Gene names

Name:	can
Ordered Locus Names:	HI_1301

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	229 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	H2CO3 = CO2 + H2O.
Cofactor	Binds 1 zinc ion per subunit.
Sequence similarities	Belongs to the beta-class carbonic anhydrase family.

Ontologies

Keywords
Ligand	Metal-binding Zinc
Molecular function	Lyase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbon utilization Inferred from direct assay Ref.2. Source: UniProtKB
Molecular_function	carbonate dehydratase activity Inferred from direct assay Ref.2. Source: UniProtKB zinc ion binding Inferred from direct assay Ref.2. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 229

229

Carbonic anhydrase 2

PRO_0000077467

Sites

Metal binding

42

1

Zinc

Metal binding

44

1

Zinc

Metal binding

98

1

Zinc

Metal binding

101

1

Zinc

Secondary structure

1

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229

Helix Strand Turn

Details...

Helix

2 – 20

19

Helix

25 – 31

7

Beta strand

37 – 42

6

Turn

43 – 45

3

Helix

49 – 53

5

Beta strand

59 – 65

7

Helix

66 – 68

3

Helix

75 – 86

12

Beta strand

92 – 100

9

Helix

102 – 108

7

Helix

116 – 129

14

Helix

131 – 135

5

Helix

139 – 141

3

Helix

142 – 159

18

Helix

162 – 169

8

Beta strand

175 – 181

7

Turn

183 – 185

3

Beta strand

188 – 197

10

Helix

198 – 212

15

Helix

217 – 219

3

Sequences

	Sequence		Length	Mass (Da)
	P45148 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4EB20478BC9EB6C9	FASTA	229	26,250
	10 20 30 40 50 60 MDKIKQLFAN NYSWAQRMKE ENSTYFKELA DHQTPHYLWI GCSDSRVPAE KLTNLEPGEL 70 80 90 100 110 120 FVHRNVANQV IHTDFNCLSV VQYAVDVLKI EHIIICGHTN CGGIHAAMAD KDLGLINNWL 130 140 150 160 170 180 LHIRDIWFKH GHLLGKLSPE KRADMLTKIN VAEQVYNLGR TSIVKSAWER GQKLSLHGWV 190 200 210 220 YDVNDGFLVD QGVMATSRET LEISYRNAIA RLSILDEENI LKKDHLENT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Identification of a novel noncatalytic bicarbonate binding site in eubacterial beta-carbonic anhydrase." Cronk J.D., Rowlett R.S., Zhang K.Y., Tu C., Endrizzi J.A., Lee J., Gareiss P.C., Preiss J.R. Biochemistry 45:4351-4361(2006) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.3 ANGSTROMS), ZINC-BINDING.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L42023 Genomic DNA. Translation: AAC22946.1.

PIR

F64170.

RefSeq

NP_439452.1. NC_000907.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2A8C	X-ray	2.30	A/B/C/D/E/F	1-229	[»]
2A8D	X-ray	2.20	A/B/C/D/E/F	1-229	[»]
3E1V	X-ray	2.80	A/B	1-229	[»]
3E1W	X-ray	2.60	A/B	1-229	[»]
3E24	X-ray	2.30	A/B	1-229	[»]
3E28	X-ray	2.50	A/B/C/D/E/F	1-229	[»]
3E2A	X-ray	2.30	A/B/C/D/E/F	1-229	[»]
3E2W	X-ray	2.30	A/B/C/D/E/F	1-229	[»]
3E2X	X-ray	2.55	A/B	1-229	[»]
3E31	X-ray	2.95	A/B	1-229	[»]
3E3F	X-ray	2.30	A/B	1-229	[»]
3E3G	X-ray	2.30	A/B/C/D/E/F	1-229	[»]
3E3I	X-ray	2.00	A/B/C/D/E/F/G/H/I/J/K/L	1-229	[»]
3MF3	X-ray	2.50	A/B/C/D/E/F	1-221	[»]

ProteinModelPortal

P45148.

ModBase

Search...

Protein-protein interaction databases

STRING

71421.HI1301.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC22946; AAC22946; HI_1301.

GeneID

950229.

KEGG

hin:HI1301.

PATRIC

20191285. VBIHaeInf48452_1353.

Phylogenomic databases

eggNOG

COG0288.

KO

K01673.

OMA

PHRIKEL.

ProtClustDB

CLSK870162.

Family and domain databases

Gene3D

3.40.1050.10. 1 hit.

InterPro

IPR001765. Carbonic_anhydrase.
IPR015892. Carbonic_anhydrase_CS.
[Graphical view]

PANTHER

PTHR11002. PTHR11002. 1 hit.

Pfam

PF00484. Pro_CA. 1 hit.
[Graphical view]

SMART

SM00947. Pro_CA. 1 hit.
[Graphical view]

SUPFAM

SSF53056. Prok_plnt_COanhd. 1 hit.

PROSITE

PS00704. PROK_CO2_ANHYDRASE_1. 1 hit.
PS00705. PROK_CO2_ANHYDRASE_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

EvolutionaryTrace

P45148.

Entry information

Entry name

CAN_HAEIN

Accession

Primary (citable) accession number: P45148

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 87 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program