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Protein

UvrABC system protein B

Gene

uvrB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

The UvrABC repair system catalyzes the recognition and processing of DNA lesions. A damage recognition complex composed of 2 UvrA and 2 UvrB subunits scans DNA for abnormalities. Upon binding of the UvrA2B2 complex to a putative damaged site, the DNA wraps around one UvrB monomer. DNA wrap is dependent on ATP binding by UvrB and probably causes local melting of the DNA helix, facilitating insertion of UvrB beta-hairpin between the DNA strands. Then UvrB probes one DNA strand for the presence of a lesion. If a lesion is found the UvrA subunits dissociate and the UvrB-DNA preincision complex is formed. This complex is subsequently bound by UvrC and the second UvrB is released. If no lesion is found, the DNA wraps around the other UvrB subunit that will check the other stand for damage.UniRule annotation

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi44 – 51ATPUniRule annotation8

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionExcision nuclease
Biological processDNA damage, DNA excision, DNA repair, SOS response
LigandATP-binding, Nucleotide-binding

Names & Taxonomyi

Protein namesi
Recommended name:
UvrABC system protein BUniRule annotation
Short name:
Protein UvrBUniRule annotation
Alternative name(s):
Excinuclease ABC subunit BUniRule annotation
Gene namesi
Name:uvrBUniRule annotation
Ordered Locus Names:HI_1247
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

P45125:
  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001383951 – 679UvrABC system protein BAdd BLAST679

Interactioni

Subunit structurei

Forms a heterotetramer with UvrA during the search for lesions. Interacts with UvrC in an incision complex.UniRule annotation

Protein-protein interaction databases

STRINGi71421.HI1247.

Structurei

3D structure databases

ProteinModelPortaliP45125.
SMRiP45125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini31 – 414Helicase ATP-bindingUniRule annotationAdd BLAST384
Domaini436 – 589Helicase C-terminalUniRule annotationAdd BLAST154
Domaini639 – 674UVRUniRule annotationAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi97 – 120Beta-hairpinAdd BLAST24

Domaini

The beta-hairpin motif is involved in DNA binding.UniRule annotation

Sequence similaritiesi

Belongs to the UvrB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCW. Bacteria.
COG0556. LUCA.
KOiK03702.
OMAiRYMHSEI.
PhylomeDBiP45125.

Family and domain databases

CDDicd00079. HELICc. 1 hit.
Gene3Di4.10.860.10. 1 hit.
HAMAPiMF_00204. UvrB. 1 hit.
InterProiView protein in InterPro
IPR006935. Helicase/UvrB_N.
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR001943. UVR_dom.
IPR036876. UVR_dom_sf.
IPR004807. UvrB.
IPR024759. UvrB_YAD/RRR_dom.
PANTHERiPTHR24029. PTHR24029. 1 hit.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF04851. ResIII. 1 hit.
PF02151. UVR. 1 hit.
PF12344. UvrB. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF46600. SSF46600. 1 hit.
SSF52540. SSF52540. 3 hits.
TIGRFAMsiTIGR00631. uvrb. 1 hit.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS50151. UVR. 1 hit.

Sequencei

Sequence statusi: Complete.

P45125-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSEKINTKPF ILHSDFRPSG DQPQAIEKLA ENLTDGLAHQ TLLGVTGSGK
60 70 80 90 100
TFTIANVIAQ LNRPAMLLAP NKTLAAQLYA EMKAFFPENA VEYFVSYYDY
110 120 130 140 150
YQPEAYVPSS DTFIEKDASI NDQIEQMRLS ATKSFLERRD TIVVASVSAI
160 170 180 190 200
YGLGDPDSYL QMMLHLQQGA IIDQRQILAK LAELQYTRND QAFQRGTFRV
210 220 230 240 250
RGEIIDIFPA ESDDRAVRIE LFDDEIERLS LFDPLTGSSF GAVPRFTIYP
260 270 280 290 300
KTHYVTPRER ILDAIENIKK ELVSRREYFI KEHKLLEEQR ISQRTQFDIE
310 320 330 340 350
MMNELGYCSG IENYSRYLSG RNEGEPPPTL FDYMPPDAIL IIDESHVTVP
360 370 380 390 400
QIGGMYRGDR SRKETLVEYG FRLPSALDNR PLRFEEFERL APQTIYVSAT
410 420 430 440 450
PGAYELEKSG SEIIDQVVRP TGLLDPLIEI RPVSIQVDDL LSEARQRADK
460 470 480 490 500
NERVLVTTLT KKMAEDLTDY LDEHGIRVRY LHSDIDTVER VEIIRDLRLG
510 520 530 540 550
EFDVLVGINL LREGLDIPEV SLVAILDADK EGFLRSERSL IQTIGRAARN
560 570 580 590 600
LNGKAILYAD SITKSMEKAI TETNRRREKQ TKYNEEHGIV PQALNKKVGE
610 620 630 640 650
LLDIGQGANQ KAKANKQRGK MAAEPTALYN APKNAKEYQQ QIKKLEQQMY
660 670
KFAQDLEFEK AAAIRDQLHQ LREQFVFDN
Length:679
Mass (Da):77,526
Last modified:November 1, 1995 - v1
Checksum:i926DF16C1B21A0C3
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22897.1.
PIRiG64112.
RefSeqiNP_439403.1. NC_000907.1.
WP_005694308.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22897; AAC22897; HI_1247.
GeneIDi950196.
KEGGihin:HI1247.
PATRICifig|71421.8.peg.1299.

Similar proteinsi

Entry informationi

Entry nameiUVRB_HAEIN
AccessioniPrimary (citable) accession number: P45125
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 25, 2017
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families