P45106 (PRMB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
50S ribosomal protein L3 glutamine methyltransferase
Short name=L3 MTase
EC=2.1.1.n15

Alternative name(s):
N5-glutamine methyltransferase PrmB

Gene names

Name:	prmB
Ordered Locus Names:	HI_1201

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	314 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Specifically methylates the 50S ribosomal protein L3 on a specific glutamine residue By similarity. HAMAP MF_02125
Catalytic activity	S-adenosyl-L-methionine + glutamine in ribosomal protein L3 = S-adenosyl-L-homocysteine + N5-methylglutamine in ribosomal protein L3. HAMAP MF_02125
Sequence similarities	Belongs to the protein N5-glutamine methyltransferase family. PrmB subfamily.

Ontologies

Keywords
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular component	intracellular Inferred from electronic annotation. Source: InterPro
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro protein methyltransferase activity Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 314	314	50S ribosomal protein L3 glutamine methyltransferase HAMAP MF_02125		PRO_0000088006

Sequences

	Sequence		Length	Mass (Da)
	P45106 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2AC862003FE05301	FASTA	314	35,590
	10 20 30 40 50 60 METSHNQELV ATILEDNVAN ELQTIQDFLR WTYSILNRSD IYFGQGHDNP WDESLQLVLS 70 80 90 100 110 120 SLHLPIDLPT ELFNSRLTPS EKETLVQLVL TRIEQRVPVA YLTNSAWFCG HEFYVDERTI 130 140 150 160 170 180 IPRSPISALI QDRFEDLISQ EPNHILDLCT GSGCIAIACA YAFPNAEVDA VDLSVDALNV 190 200 210 220 230 240 AEINISRHQL EHRVFPIQSN LFENILGQKY DLIVTNPPYV DEEDLADMPE EFHFEPELAL 250 260 270 280 290 300 GSGSDGLNIT KQILKQAPDY LTENGVLVCE VGNSMISLIE QYPDVPFEWV ELKNGGLGVF 310 AIQRKDLVKY HDLF

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22855.1.
PIR	F64189.
RefSeq	NP_439357.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P45106.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	950156.
GenomeReviews	Gene locus HI_1201 in contig L42023_GR.
KEGG	hin:HI1201.
NMPDR	fig|71421.1.peg.1148.
PATRIC	20191081. VBIHaeInf48452_1253.
TIGR	HI_1201.
Phylogenomic databases
HOGENOM	HBG732041.
OMA	VGNSQVH.
ProtClustDB	PRK11805.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1201-MONOMER.
Family and domain databases
HAMAP	MF_02125. L3_methyltr_PrmB. [Tree]
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR004556. Modification_methylase_HemK. IPR017127. Ribosome_L3_Gln-N5_MeTrfase. IPR007848. Small_mtfrase. [Graphical view]
KO	K07320.
Pfam	PF05175. MTS. 1 hit. [Graphical view]
PIRSF	PIRSF037167. Mtase_YfcB_prd. 1 hit.
TIGRFAMs	TIGR00536. HemK_fam. 1 hit. TIGR03533. L3_gln_methyl. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PRMB_HAEIN

Accession

Primary (citable) accession number: P45106

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program