P45100 (RLMM_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosomal RNA large subunit methyltransferase M
EC=2.1.1.186

Alternative name(s):
23S rRNA (cytidine2498-2'-O)-methyltransferase
23S rRNA 2'-O-ribose methyltransferase RlmM

Gene names

Name:	rlmM
Ordered Locus Names:	HI_1195

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the 2'-O-methylation at nucleotide C2498 in 23S rRNA By similarity. HAMAP-Rule MF_01551
Catalytic activity	S-adenosyl-L-methionine + cytidine(2498) in 23S rRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(2498) in 23S rRNA. HAMAP-Rule MF_01551
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm Potential HAMAP-Rule MF_01551.
Sequence similarities	Belongs to the methyltransferase superfamily. RlmE family. RlmM subfamily.
Sequence caution	The sequence AAC22849.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	rRNA processing
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	rRNA processing Inferred from electronic annotation. Source: HAMAP
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	S-adenosylmethionine-dependent methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 363	363	Ribosomal RNA large subunit methyltransferase M HAMAP-Rule MF_01551		PRO_0000070406
Regions
	Region	227 – 230	4	S-adenosyl-L-methionine binding By similarity
Sites
	Active site	313	1	Proton acceptor By similarity
	Binding site	194	1	S-adenosyl-L-methionine By similarity
	Binding site	246	1	S-adenosyl-L-methionine By similarity
	Binding site	266	1	S-adenosyl-L-methionine By similarity
	Binding site	284	1	S-adenosyl-L-methionine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45100 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: DC7B76D1B192D524	FASTA	363	41,563
	10 20 30 40 50 60 MNKLALYCRP GFEKEVAAEI TDQASHLGVF GFARVQDNSG YVIFECYQPD EADRLARDIP 70 80 90 100 110 120 FNRLIFARQM MVISDLLEDL DPADRISPIV VAFEELSQQV NFAQSSELFV ETADTNEAKE 130 140 150 160 170 180 LSTFCRKFTV PLRQALKKQG WLSAKASQKC GQFLHCFFVK PNCCYVGYSY VDNHSSHFMG 190 200 210 220 230 240 IPRLKFPADA PSRSTLKLEE AILTFIPRKE ENKRLNENMI GVDLGACPGG WTYQLVKRGL 250 260 270 280 290 300 FVYAVDHGKM AASLHDTGRI EHCAEDGFKF QPPKRKKVDW LVCDMVEQPS RISLLIGKWL 310 320 330 340 350 360 LNGWCRETIF NLKLPMKKRY QEVILCLENL AVMLAEQNLN FDIQAKHLYH DREEITVHIA LKP

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22849.1. Different initiation.
PIR	H64168.
RefSeq	NP_439351.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P45100.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1195m.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22849; AAC22849; HI_1195.
GeneID	950147.
KEGG	hin:HI1195m.
PATRIC	20191069. VBIHaeInf48452_1247.
Phylogenomic databases
eggNOG	COG2933.
KO	K06968.
OMA	VIFECYQ.
ProtClustDB	PRK11760.
Family and domain databases
HAMAP	MF_01551. 23SrRNA_methyltr_M.
InterPro	IPR002877. rRNA_MeTrfase_FtsJ_dom. IPR011224. rRNA_MeTrfase_M. [Graphical view]
Pfam	PF01728. FtsJ. 1 hit. [Graphical view]
PIRSF	PIRSF028774. UCP028774. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RLMM_HAEIN

Accession

Primary (citable) accession number: P45100

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program