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Protein

Cysteine synthase

Gene

cysK

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

O-acetyl-L-serine + hydrogen sulfide = L-cysteine + acetate.

Cofactori

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Serine acetyltransferase (cysE)
  2. Cysteine synthase (cysK)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei7Allosteric inhibitorBy similarity1
Binding sitei72Pyridoxal phosphateBy similarity1
Binding sitei268Allosteric inhibitor; via amide nitrogenBy similarity1
Binding sitei272Pyridoxal phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BRENDAi2.5.1.47. 2529.
UniPathwayiUPA00136; UER00200.

Names & Taxonomyi

Protein namesi
Recommended name:
Cysteine synthase (EC:2.5.1.47)
Short name:
CSase
Alternative name(s):
O-acetylserine (thiol)-lyase
Short name:
OAS-TL
O-acetylserine sulfhydrylase
Gene namesi
Name:cysK
Ordered Locus Names:HI_1103
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL1075088.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001670901 – 316Cysteine synthaseAdd BLAST316

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei42N6-(pyridoxal phosphate)lysineBy similarity1

Proteomic databases

PRIDEiP45040.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI1103.

Chemistry databases

BindingDBiP45040.

Structurei

Secondary structure

1316
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi7 – 10Combined sources4
Beta strandi16 – 18Combined sources3
Beta strandi20 – 24Combined sources5
Beta strandi28 – 32Combined sources5
Helixi37 – 39Combined sources3
Helixi42 – 55Combined sources14
Beta strandi64 – 68Combined sources5
Helixi72 – 84Combined sources13
Beta strandi88 – 93Combined sources6
Beta strandi94 – 97Combined sources4
Helixi98 – 106Combined sources9
Beta strandi110 – 114Combined sources5
Helixi116 – 118Combined sources3
Helixi119 – 133Combined sources15
Turni135 – 137Combined sources3
Beta strandi138 – 140Combined sources3
Turni143 – 145Combined sources3
Helixi148 – 155Combined sources8
Helixi157 – 164Combined sources8
Turni165 – 167Combined sources3
Beta strandi169 – 175Combined sources7
Beta strandi177 – 179Combined sources3
Helixi180 – 191Combined sources12
Beta strandi198 – 204Combined sources7
Helixi209 – 215Combined sources7
Helixi240 – 242Combined sources3
Beta strandi245 – 249Combined sources5
Helixi251 – 265Combined sources15
Helixi271 – 284Combined sources14
Helixi287 – 289Combined sources3
Beta strandi293 – 298Combined sources6
Helixi302 – 305Combined sources4
Turni308 – 310Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y7LX-ray1.55A1-316[»]
3IQGX-ray1.90X1-316[»]
3IQHX-ray1.90X1-316[»]
3IQIX-ray1.70X1-316[»]
4HO1X-ray1.86X1-316[»]
4LI3X-ray2.59X1-316[»]
4NU8X-ray2.07X1-316[»]
4OREX-ray2.20X1-316[»]
4ZU1X-ray2.20X1-316[»]
4ZU6X-ray2.03X1-316[»]
5DBEX-ray2.25X1-316[»]
5DBHX-ray1.98X1-316[»]
ProteinModelPortaliP45040.
SMRiP45040.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP45040.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni177 – 181Pyridoxal phosphate bindingBy similarity5

Sequence similaritiesi

Phylogenomic databases

eggNOGiENOG4105C6T. Bacteria.
COG0031. LUCA.
KOiK01738.
OMAiVKCRIGS.
PhylomeDBiP45040.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45040-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAIYADNSYS IGNTPLVRLK HFGHNGNVVV KIEGRNPSYS VKCRIGANMV
60 70 80 90 100
WQAEKDGTLT KGKEIVDATS GNTGIALAYV AAARGYKITL TMPETMSLER
110 120 130 140 150
KRLLCGLGVN LVLTEGAKGM KGAIAKAEEI VASDPSRYVM LKQFENPANP
160 170 180 190 200
QIHRETTGPE IWKDTDGKVD VVVAGVGTGG SITGISRAIK LDFGKQITSV
210 220 230 240 250
AVEPVESPVI SQTLAGEEVK PGPHKIQGIG AGFIPKNLDL SIIDRVETVD
260 270 280 290 300
SDTALATARR LMAEEGILAG ISSGAAVAAA DRLAKLPEFA DKLIVVILPS
310
ASERYLSTAL FEGIEG
Length:316
Mass (Da):33,384
Last modified:November 1, 1995 - v1
Checksum:iCA753FC79BBD05BD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22758.1.
PIRiF64182.
RefSeqiNP_439260.1. NC_000907.1.
WP_005693427.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22758; AAC22758; HI_1103.
GeneIDi950077.
KEGGihin:HI1103.
PATRICi20190873. VBIHaeInf48452_1149.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22758.1.
PIRiF64182.
RefSeqiNP_439260.1. NC_000907.1.
WP_005693427.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Y7LX-ray1.55A1-316[»]
3IQGX-ray1.90X1-316[»]
3IQHX-ray1.90X1-316[»]
3IQIX-ray1.70X1-316[»]
4HO1X-ray1.86X1-316[»]
4LI3X-ray2.59X1-316[»]
4NU8X-ray2.07X1-316[»]
4OREX-ray2.20X1-316[»]
4ZU1X-ray2.20X1-316[»]
4ZU6X-ray2.03X1-316[»]
5DBEX-ray2.25X1-316[»]
5DBHX-ray1.98X1-316[»]
ProteinModelPortaliP45040.
SMRiP45040.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1103.

Chemistry databases

BindingDBiP45040.
ChEMBLiCHEMBL1075088.

Proteomic databases

PRIDEiP45040.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22758; AAC22758; HI_1103.
GeneIDi950077.
KEGGihin:HI1103.
PATRICi20190873. VBIHaeInf48452_1149.

Phylogenomic databases

eggNOGiENOG4105C6T. Bacteria.
COG0031. LUCA.
KOiK01738.
OMAiVKCRIGS.
PhylomeDBiP45040.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00200.
BRENDAi2.5.1.47. 2529.

Miscellaneous databases

EvolutionaryTraceiP45040.
PROiP45040.

Family and domain databases

InterProiIPR005856. Cys_synth.
IPR005859. CysK.
IPR001216. P-phosphate_BS.
IPR001926. TrpB-like_PLP-dep.
[Graphical view]
PfamiPF00291. PALP. 1 hit.
[Graphical view]
SUPFAMiSSF53686. SSF53686. 1 hit.
TIGRFAMsiTIGR01139. cysK. 1 hit.
TIGR01136. cysKM. 1 hit.
PROSITEiPS00901. CYS_SYNTHASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYSK_HAEIN
AccessioniPrimary (citable) accession number: P45040
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 130 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Allosteric enzyme, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.