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Reviewed, UniProtKB/Swiss-Prot P45017 (NRFA_HAEIN)

Last modified June 16, 2009. Version 83. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    Cytochrome c-552
    EC=1.7.2.2
Alternative name(s):
    Ammonia-forming cytochrome c nitrite reductase
      Short name=Cytochrome c nitrite reductase
Gene names
Name: nrfA
Ordered Locus Names: HI1069
OrganismHaemophilus influenzae [Complete proteome] [HAMAP]
Taxonomic identifier727 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

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Protein attributes

Sequence length538 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

Plays a role in nitrite reduction By similarity.

Catalytic activity

NH3 + 2 H2O + 6 ferricytochrome c = nitrite + 6 ferrocytochrome c + 7 H+. HAMAP MF_01182

Cofactor

Binds 1 calcium ion per monomer By similarity.

Binds 5 heme groups covalently per monomer By similarity.

Pathway

Nitrogen metabolism; nitrate reduction (assimilation). HAMAP MF_01182

Subcellular location

Periplasm By similarity.

Sequence similarities

Belongs to the cytochrome c-552 family.

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Ontologies

Keywords
   Biological processElectron transport
Transport
   Cellular componentPeriplasm
   DomainSignal
   LigandCalcium
Heme
Iron
Metal-binding
   Molecular functionOxidoreductase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological processelectron transport chain

Inferred from electronic annotation. Source: UniProtKB-KW

nitrogen compound metabolic process

Inferred from electronic annotation. Source: HAMAP

transport

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functioncalcium ion binding

Inferred from electronic annotation. Source: UniProtKB-KW

heme binding

Inferred from electronic annotation. Source: InterPro

nitrite reductase (cytochrome, ammonia-forming) activity

Inferred from electronic annotation. Source: HAMAP

Complete GO annotation...

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 5555 Potential
Chain56 – 538483Cytochrome c-552 HAMAP MF_01182
PRO_0000006579

Sites

Metal binding1331Iron (heme 3 axial ligand) By similarity
Metal binding1651Iron (heme 1 axial ligand) By similarity
Metal binding2031Iron (heme 2 axial ligand) By similarity
Metal binding2681Iron (heme 3 axial ligand) By similarity
Metal binding2701Calcium By similarity
Metal binding2711Calcium; via carbonyl oxygen By similarity
Metal binding3161Calcium; via carbonyl oxygen By similarity
Metal binding3181Calcium By similarity
Metal binding3301Iron (heme 5 axial ligand) By similarity
Metal binding3411Iron (heme 4 axial ligand) By similarity
Metal binding3561Iron (heme 2 axial ligand) By similarity
Metal binding3731Iron (heme 5 axial ligand) By similarity
Metal binding4481Iron (heme 4 axial ligand) By similarity
Binding site1611Heme 1 (covalent) By similarity
Binding site1641Heme 1 (covalent) By similarity
Binding site1991Heme 2 (covalent) By similarity
Binding site2021Heme 2 (covalent) By similarity
Binding site2641Heme 3 (covalent) By similarity
Binding site2671Heme 3 (covalent) By similarity
Binding site2711Substrate By similarity
Binding site3191Substrate By similarity
Binding site3371Heme 4 (covalent) By similarity
Binding site3401Heme 4 (covalent) By similarity
Binding site3691Heme 5 (covalent) By similarity
Binding site3721Heme 5 (covalent) By similarity

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Sequences

Sequence LengthMass (Da)Tools
P45017-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 48E64B46F2FB9CBB

FASTA53860,400
        10         20         30         40         50         60 
MKIYLRFVWI LIIILNFLLN LFITTNGVII VNAFKKSLIV AASFASLSLF NSATAELVYK 

        70         80         90        100        110        120 
PLEQPVEPAK PDLKIESVNE KFAEKYPNQY NSWRSTANGD GENIIYADEE NPRLIVLWGG 

       130        140        150        160        170        180 
YAFAKEYNAP RGHFYAVTDV RNILRTGAPK TANDGPQAMA CWTCKGPDVP RLIAEWGEKD 

       190        200        210        220        230        240 
YFNAKWAKGG PEIVNSIGCA DCHDTTSKDF AEGKPALRIA RPHVLRALDA LEKATAEKDK 

       250        260        270        280        290        300 
AEGRPHNNLS FNTAARTEKR AEICANCHVE YYFAGDIKQV TFPWDNGQTV DDIEKYYDDI 

       310        320        330        340        350        360 
GFTDWTHSLS KAPMLKAQHP DFEIWSLGMH GKNGVTCVDC HMPKVQGADG KVYTDHQIQN 

       370        380        390        400        410        420 
PFEAFDSTCA NCHDQSKEKL RDIVTSRKKE VKDVMGRLED QVVKAHFEAK EAWDAGATKK 

       430        440        450        460        470        480 
EMEAALMDIR HAQWRWDYTA ASHGGHMHAP EVVLRVLASG LDKVADARTK LAVILTKHGV 

       490        500        510        520        530 
KTPVQIPDIS TADKAWKVMG IDIEKERKAK EEFLKTVVPQ WEQQAREKGL LVDPPAQK

« Hide

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Cross-references

Sequence databases

L42023 Genomic DNA. Translation: AAC22727.1.
PIRC64181.
RefSeqNP_439227.3.

3D structure databases

HSSPHSSP built from PDB template 1GU6 based on UniProtKB P32050.
ModBaseSearch...

Genome annotation databases

GeneID950047.
GenomeReviewsGene locus HI1069 in contig L42023_GR.
KEGGhin:HI1069.
NMPDRfig|71421.1.peg.1028.
TIGRHI1069.

Phylogenomic databases

HOGENOMP45017.
OMAP45017. CHDQSKE.

Enzyme and pathway databases

BioCycHINF71421:HI_1069-MON.
BRENDA1.7.2.2. 109.

Family and domain databases

HAMAPMF_01182.
[Tree]
InterProIPR003321. Cyt_c552.
IPR017570. Cyt_c_NO2Rdtase_formate-dep.
IPR011031. Multihaem_cyt.
[Graphical view]
PfamPF02335. Cytochrom_C552. 1 hit.
[Graphical view]
PIRSFPIRSF000243. Cyt_c552. 1 hit.
TIGRFAMsTIGR03152. cyto_c552_HCOOH. 1 hit.
PROSITEPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry nameNRFA_HAEIN
AccessionPrimary (citable) accession number: P45017
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 16, 2009
This is version 83 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents