ID   LPXB_HAEIN              Reviewed;         390 AA.
AC   P45011; P94807;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1997, sequence version 2.
DT   27-MAR-2024, entry version 122.
DE   RecName: Full=Lipid-A-disaccharide synthase;
DE            EC=2.4.1.182;
GN   Name=lpxB; OrderedLocusNames=HI_1060;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
RN   [2]
RP   SEQUENCE REVISION.
RA   White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D.;
RL   Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases.
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=RM 7004 / Serotype B;
RX   PubMed=8917090; DOI=10.1016/0378-1119(96)00139-4;
RA   Servos S., Khan S., Maskell D.;
RT   "Cloning and expression of genes encoding lipid A biosynthesis from
RT   Haemophilus influenzae type b.";
RL   Gene 175:137-141(1996).
CC   -!- FUNCTION: Condensation of UDP-2,3-diacylglucosamine and 2,3-
CC       diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor
CC       of lipid A, a phosphorylated glycolipid that anchors the
CC       lipopolysaccharide to the outer membrane of the cell.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a lipid X + a UDP-2-N,3-O-bis[(3R)-3-hydroxyacyl]-alpha-D-
CC         glucosamine = a lipid A disaccharide + H(+) + UDP;
CC         Xref=Rhea:RHEA:67828, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223,
CC         ChEBI:CHEBI:137748, ChEBI:CHEBI:176338, ChEBI:CHEBI:176343;
CC         EC=2.4.1.182;
CC   -!- PATHWAY: Bacterial outer membrane biogenesis; LPS lipid A biosynthesis.
CC   -!- SIMILARITY: Belongs to the LpxB family. {ECO:0000305}.
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DR   EMBL; L42023; AAC22715.1; -; Genomic_DNA.
DR   EMBL; X87416; CAA60866.1; -; Genomic_DNA.
DR   RefSeq; NP_439218.1; NC_000907.1.
DR   AlphaFoldDB; P45011; -.
DR   SMR; P45011; -.
DR   STRING; 71421.HI_1060; -.
DR   CAZy; GT19; Glycosyltransferase Family 19.
DR   EnsemblBacteria; AAC22715; AAC22715; HI_1060.
DR   KEGG; hin:HI_1060; -.
DR   PATRIC; fig|71421.8.peg.1104; -.
DR   eggNOG; COG0763; Bacteria.
DR   HOGENOM; CLU_036577_3_0_6; -.
DR   OrthoDB; 9801642at2; -.
DR   PhylomeDB; P45011; -.
DR   BioCyc; HINF71421:G1GJ1-1097-MONOMER; -.
DR   UniPathway; UPA00973; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0008915; F:lipid-A-disaccharide synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005543; F:phospholipid binding; IBA:GO_Central.
DR   GO; GO:0009245; P:lipid A biosynthetic process; IBA:GO_Central.
DR   HAMAP; MF_00392; LpxB; 1.
DR   InterPro; IPR003835; Glyco_trans_19.
DR   NCBIfam; TIGR00215; lpxB; 1.
DR   PANTHER; PTHR30372; LIPID-A-DISACCHARIDE SYNTHASE; 1.
DR   PANTHER; PTHR30372:SF7; LIPID-A-DISACCHARIDE SYNTHASE; 1.
DR   Pfam; PF02684; LpxB; 1.
DR   SUPFAM; SSF53756; UDP-Glycosyltransferase/glycogen phosphorylase; 1.
PE   3: Inferred from homology;
KW   Glycosyltransferase; Lipid A biosynthesis; Lipid biosynthesis;
KW   Lipid metabolism; Reference proteome; Transferase.
FT   CHAIN           1..390
FT                   /note="Lipid-A-disaccharide synthase"
FT                   /id="PRO_0000190168"
FT   CONFLICT        342
FT                   /note="V -> A (in Ref. 3; CAA60866)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        350
FT                   /note="A -> S (in Ref. 3; CAA60866)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        387..388
FT                   /note="KE -> TK (in Ref. 3; CAA60866)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   390 AA;  43637 MW;  690BEC98ECFEECCD CRC64;
     MNKTNPTIAL VAGEVSGDIL GAGLIRQLKA HYPNARFIGI AGPRMLAEGC ETLVDMEELS
     VMGLAEILKH LPRLLKIRKN VIQTMLQEKP DVYIGIDAPD FNLDVELKLK ANGIKTIHYV
     SPSVWAWRQN RIHKIAKATH QVLAFLPFEK AFYDKFNVPC RFIGHTMADA IPLKPNRAEA
     CQTLQIDPAQ RYLAILVGSR GSEVEFLAEP FLKTALLLKE QFPDLQFLVP LVNEKRRIQF
     ETIKAKITPN LDLHLIDGNA RQAMIAADAT LLASGTAALE AMLCKSPMVV GYRMKPLTYF
     LAKRLVKTDY ISLPNLLANE MLVPEMIQEE CTPELLAEKL SVYLSDDESA VKNRHVLIQH
     FTDLHQKIQC NADKQAAQAV IDLLEGKENV
//