P45011 (LPXB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Lipid-A-disaccharide synthase
EC=2.4.1.182

Gene names

Name:	lpxB
Ordered Locus Names:	HI_1060

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Condensation of UDP-2,3-diacylglucosamine and 2,3-diacylglucosamine-1-phosphate to form lipid A disaccharide, a precursor of lipid A, a phosphorylated glycolipid that anchors the lipopolysaccharide to the outer membrane of the cell. HAMAP-Rule MF_00392
Catalytic activity	UDP-2,3-bis(3-hydroxytetradecanoyl)glucosamine + 2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate = UDP + 2,3-bis(3-hydroxytetradecanoyl)-D-glucosaminyl-1,6-beta-D-2,3-bis(3-hydroxytetradecanoyl)-beta-D-glucosaminyl 1-phosphate. HAMAP-Rule MF_00392
Pathway	Glycolipid biosynthesis; lipid IV(A) biosynthesis; lipid IV(A) from (3R)-3-hydroxytetradecanoyl-[acyl-carrier-protein] and UDP-N-acetyl-alpha-D-glucosamine: step 5/6. HAMAP-Rule MF_00392
Sequence similarities	Belongs to the LpxB family.

Ontologies

Keywords
Biological process	Lipid A biosynthesis Lipid biosynthesis Lipid metabolism
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	lipid A biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	lipid-A-disaccharide synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Lipid-A-disaccharide synthase HAMAP-Rule MF_00392		PRO_0000190168
Experimental info
	Sequence conflict	342	1	V → A in CAA60866. Ref.3
	Sequence conflict	350	1	A → S in CAA60866. Ref.3
	Sequence conflict	387 – 388	2	KE → TK in CAA60866. Ref.3

Sequences

	Sequence		Length	Mass (Da)
	P45011 [UniParc]. Last modified November 1, 1997. Version 2. Checksum: 690BEC98ECFEECCD	FASTA	390	43,637
	10 20 30 40 50 60 MNKTNPTIAL VAGEVSGDIL GAGLIRQLKA HYPNARFIGI AGPRMLAEGC ETLVDMEELS 70 80 90 100 110 120 VMGLAEILKH LPRLLKIRKN VIQTMLQEKP DVYIGIDAPD FNLDVELKLK ANGIKTIHYV 130 140 150 160 170 180 SPSVWAWRQN RIHKIAKATH QVLAFLPFEK AFYDKFNVPC RFIGHTMADA IPLKPNRAEA 190 200 210 220 230 240 CQTLQIDPAQ RYLAILVGSR GSEVEFLAEP FLKTALLLKE QFPDLQFLVP LVNEKRRIQF 250 260 270 280 290 300 ETIKAKITPN LDLHLIDGNA RQAMIAADAT LLASGTAALE AMLCKSPMVV GYRMKPLTYF 310 320 330 340 350 360 LAKRLVKTDY ISLPNLLANE MLVPEMIQEE CTPELLAEKL SVYLSDDESA VKNRHVLIQH 370 380 390 FTDLHQKIQC NADKQAAQAV IDLLEGKENV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D. Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	"Cloning and expression of genes encoding lipid A biosynthesis from Haemophilus influenzae type b." Servos S., Khan S., Maskell D. Gene 175:137-141(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: RM 7004 / Serotype B.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22715.1. X87416 Genomic DNA. Translation: CAA60866.1.
RefSeq	NP_439218.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P45011.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI1060.
Protein family/group databases
CAZy	GT19. Glycosyltransferase Family 19.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22715; AAC22715; HI_1060.
GeneID	950031.
KEGG	hin:HI1060.
PATRIC	20190783. VBIHaeInf48452_1104.
Phylogenomic databases
eggNOG	COG0763.
KO	K00748.
OMA	PTVWAWR.
ProtClustDB	PRK00025.
Enzyme and pathway databases
UniPathway	UPA00359; UER00481.
Family and domain databases
HAMAP	MF_00392. LpxB.
InterPro	IPR003835. Glyco_trans_19. [Graphical view]
Pfam	PF02684. LpxB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00215. lpxB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

LPXB_HAEIN

Accession

Primary (citable) accession number: P45011
Secondary accession number(s): P94807

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program