P45000 (MTH5_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase HindV
Short name=M.HindV
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase HindV

Gene names

Name:	hindVM
Ordered Locus Names:	HI_1041

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	304 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HindV endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 304	304	Modification methylase HindV		PRO_0000087881
Sites
	Active site	75	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	P45000 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 03DA1EAB27C84BBD	FASTA	304	34,366
	10 20 30 40 50 60 MKCVDLFSGC GGLSLGFELA GFEICAAFEN WEKAIEIYKN NFSHPIYNID LRNEKEAVEK 70 80 90 100 110 120 IKKYSPDLIM GGPPCQDFSS AGKRDISLGR ADLTYSFANI VCNIRPKWFV MENVEQIKKS 130 140 150 160 170 180 HILQDIINQF IDFGYGLTSA ILDASYCGVP QSRTRFSLIG KLNSEHNFLI PTLSRKLSDK 190 200 210 220 230 240 PMTVRDYLGN SLNLEFYYRH PRNYNRRGIF SIDEPSPTIR GVNRPIPKGY NINSCDPKGV 250 260 270 280 290 300 ELAKVRPLTT IERSYIQTFP KSFLFSGTKT DLEQMIGNAV PVNLAKFVAS AIINFEKEPI RSMG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22700.1.
PIR	C64109.
RefSeq	NP_439200.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P45000.
ModBase	Search...
Protein family/group databases
REBASE	3574. M.HindV.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	950661.
GenomeReviews	Gene locus HI_1041 in contig L42023_GR.
KEGG	hin:HI1041.
NMPDR	fig|71421.1.peg.1002.
PATRIC	20190745. VBIHaeInf48452_1085.
TIGR	HI_1041.
Phylogenomic databases
HOGENOM	HBG422323.
OMA	RGVNRPI.
ProtClustDB	CLSK892394.
Enzyme and pathway databases
BioCyc	HINF71421:HI_1041-MONOMER.
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
KO	K00558.
PANTHER	PTHR10629. C5_DNA_meth. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. Dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTH5_HAEIN

Accession

Primary (citable) accession number: P45000

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program