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Protein

Modification methylase HindV

Gene

hindVM

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HindV endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei75 – 751PROSITE-ProRule annotation

GO - Molecular functioni

  1. DNA (cytosine-5-)-methyltransferase activity Source: UniProtKB-EC
  2. DNA binding Source: InterPro

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3574. M.HindV.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase HindV (EC:2.1.1.37)
Short name:
M.HindV
Alternative name(s):
Cytosine-specific methyltransferase HindV
Gene namesi
Name:hindVM
Ordered Locus Names:HI_1041
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 304304Modification methylase HindVPRO_0000087881Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI1041.

Structurei

3D structure databases

ProteinModelPortaliP45000.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 299299SAM-dependent MTase C5-typePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.PROSITE-ProRule annotation
Contains 1 SAM-dependent MTase C5-type domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0270.
KOiK00558.
OMAiRGVNRPI.
OrthoDBiEOG664CK9.
PhylomeDBiP45000.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P45000-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKCVDLFSGC GGLSLGFELA GFEICAAFEN WEKAIEIYKN NFSHPIYNID
60 70 80 90 100
LRNEKEAVEK IKKYSPDLIM GGPPCQDFSS AGKRDISLGR ADLTYSFANI
110 120 130 140 150
VCNIRPKWFV MENVEQIKKS HILQDIINQF IDFGYGLTSA ILDASYCGVP
160 170 180 190 200
QSRTRFSLIG KLNSEHNFLI PTLSRKLSDK PMTVRDYLGN SLNLEFYYRH
210 220 230 240 250
PRNYNRRGIF SIDEPSPTIR GVNRPIPKGY NINSCDPKGV ELAKVRPLTT
260 270 280 290 300
IERSYIQTFP KSFLFSGTKT DLEQMIGNAV PVNLAKFVAS AIINFEKEPI

RSMG
Length:304
Mass (Da):34,366
Last modified:November 1, 1995 - v1
Checksum:i03DA1EAB27C84BBD
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22700.1.
PIRiC64109.
RefSeqiNP_439200.1. NC_000907.1.
WP_010869113.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22700; AAC22700; HI_1041.
GeneIDi950661.
KEGGihin:HI1041.
PATRICi20190745. VBIHaeInf48452_1085.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22700.1.
PIRiC64109.
RefSeqiNP_439200.1. NC_000907.1.
WP_010869113.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP45000.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI1041.

Protein family/group databases

REBASEi3574. M.HindV.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22700; AAC22700; HI_1041.
GeneIDi950661.
KEGGihin:HI1041.
PATRICi20190745. VBIHaeInf48452_1085.

Phylogenomic databases

eggNOGiCOG0270.
KOiK00558.
OMAiRGVNRPI.
OrthoDBiEOG664CK9.
PhylomeDBiP45000.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00105. C5METTRFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00675. dcm. 1 hit.
PROSITEiPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiMTH5_HAEIN
AccessioniPrimary (citable) accession number: P45000
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 7, 2015
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.