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P45000 (MTH5_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 90. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase HindV

Short name=M.HindV
EC=2.1.1.37
Alternative name(s):
Cytosine-specific methyltransferase HindV
Gene names
Name:hindVM
Ordered Locus Names:HI_1041
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length304 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence GRCGYC, causes specific methylation on C-? on both strands, and protects the DNA from cleavage by the HindV endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.

Sequence similarities

Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family.

Contains 1 SAM-dependent MTase C5-type domain.

Ontologies

Keywords
   Biological processRestriction system
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionDNA (cytosine-5-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-EC

DNA binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 304304Modification methylase HindV
PRO_0000087881

Regions

Domain1 – 299299SAM-dependent MTase C5-type

Sites

Active site751 By similarity

Sequences

Sequence LengthMass (Da)Tools
P45000 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 03DA1EAB27C84BBD

FASTA30434,366
        10         20         30         40         50         60 
MKCVDLFSGC GGLSLGFELA GFEICAAFEN WEKAIEIYKN NFSHPIYNID LRNEKEAVEK 

        70         80         90        100        110        120 
IKKYSPDLIM GGPPCQDFSS AGKRDISLGR ADLTYSFANI VCNIRPKWFV MENVEQIKKS 

       130        140        150        160        170        180 
HILQDIINQF IDFGYGLTSA ILDASYCGVP QSRTRFSLIG KLNSEHNFLI PTLSRKLSDK 

       190        200        210        220        230        240 
PMTVRDYLGN SLNLEFYYRH PRNYNRRGIF SIDEPSPTIR GVNRPIPKGY NINSCDPKGV 

       250        260        270        280        290        300 
ELAKVRPLTT IERSYIQTFP KSFLFSGTKT DLEQMIGNAV PVNLAKFVAS AIINFEKEPI 


RSMG 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22700.1.
PIRC64109.
RefSeqNP_439200.1. NC_000907.1.

3D structure databases

ProteinModelPortalP45000.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI1041.

Protein family/group databases

REBASE3574. M.HindV.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22700; AAC22700; HI_1041.
GeneID950661.
KEGGhin:HI1041.
PATRIC20190745. VBIHaeInf48452_1085.

Phylogenomic databases

eggNOGCOG0270.
KOK00558.
OMARGVNRPI.
OrthoDBEOG664CK9.
PhylomeDBP45000.

Family and domain databases

Gene3D3.40.50.150. 1 hit.
InterProIPR018117. C5_DNA_meth_AS.
IPR001525. C5_MeTfrase.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PANTHERPTHR10629. PTHR10629. 1 hit.
PfamPF00145. DNA_methylase. 1 hit.
[Graphical view]
PRINTSPR00105. C5METTRFRASE.
SUPFAMSSF53335. SSF53335. 1 hit.
TIGRFAMsTIGR00675. dcm. 1 hit.
PROSITEPS00094. C5_MTASE_1. 1 hit.
PS00095. C5_MTASE_2. 1 hit.
PS51679. SAM_MT_C5. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTH5_HAEIN
AccessionPrimary (citable) accession number: P45000
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names