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Protein

Immunoglobulin A1 protease autotransporter

Gene

iga

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.

Catalytic activityi

Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei288 – 2881Curated

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi3.4.21.72. 2529.

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin A1 protease autotransporter (EC:3.4.21.72)
Cleaved into the following 2 chains:
Immunoglobulin A1 protease
Short name:
IGA1 protease
Alternative name(s):
Helper peptide
Gene namesi
Name:iga
Synonyms:iga1
Ordered Locus Names:HI_0990
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

Immunoglobulin A1 protease translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2525Sequence analysisAdd
BLAST
Chaini26 – 16941669Immunoglobulin A1 protease autotransporterPRO_0000387598Add
BLAST
Chaini26 – 1014989Immunoglobulin A1 proteasePRO_0000026958Add
BLAST
Chaini1015 – 1694680Immunoglobulin A1 protease translocatorSequence analysisPRO_0000026959Add
BLAST

Keywords - PTMi

Zymogen

Miscellaneous databases

PMAP-CutDBP44969.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0990.

Structurei

Secondary structure

1
1694
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi30 – 323Combined sources
Helixi34 – 429Combined sources
Beta strandi55 – 573Combined sources
Beta strandi63 – 697Combined sources
Helixi77 – 793Combined sources
Turni82 – 843Combined sources
Beta strandi88 – 914Combined sources
Beta strandi94 – 974Combined sources
Beta strandi106 – 1094Combined sources
Beta strandi111 – 1133Combined sources
Helixi130 – 1334Combined sources
Beta strandi134 – 1396Combined sources
Beta strandi148 – 1503Combined sources
Helixi154 – 1629Combined sources
Beta strandi169 – 1724Combined sources
Helixi191 – 1933Combined sources
Turni195 – 1973Combined sources
Beta strandi200 – 2056Combined sources
Beta strandi207 – 21812Combined sources
Beta strandi229 – 2368Combined sources
Beta strandi243 – 2464Combined sources
Beta strandi248 – 2514Combined sources
Beta strandi257 – 2615Combined sources
Helixi273 – 2753Combined sources
Beta strandi291 – 2966Combined sources
Turni297 – 3004Combined sources
Beta strandi301 – 31111Combined sources
Helixi313 – 3164Combined sources
Beta strandi319 – 3246Combined sources
Helixi327 – 33711Combined sources
Beta strandi338 – 35417Combined sources
Beta strandi357 – 3626Combined sources
Beta strandi365 – 3684Combined sources
Beta strandi376 – 3783Combined sources
Helixi379 – 3846Combined sources
Beta strandi388 – 40114Combined sources
Beta strandi410 – 4123Combined sources
Beta strandi414 – 4229Combined sources
Beta strandi426 – 4305Combined sources
Beta strandi432 – 4343Combined sources
Beta strandi439 – 4424Combined sources
Beta strandi451 – 46212Combined sources
Beta strandi465 – 4673Combined sources
Beta strandi471 – 4744Combined sources
Beta strandi476 – 4816Combined sources
Beta strandi491 – 4933Combined sources
Beta strandi497 – 4993Combined sources
Beta strandi504 – 5107Combined sources
Helixi516 – 5183Combined sources
Beta strandi519 – 5213Combined sources
Beta strandi526 – 5294Combined sources
Beta strandi535 – 5384Combined sources
Beta strandi541 – 5444Combined sources
Beta strandi547 – 5504Combined sources
Beta strandi558 – 5625Combined sources
Helixi571 – 5733Combined sources
Beta strandi577 – 5793Combined sources
Beta strandi600 – 6023Combined sources
Turni603 – 6064Combined sources
Beta strandi607 – 6115Combined sources
Beta strandi630 – 6389Combined sources
Helixi639 – 65315Combined sources
Beta strandi660 – 6634Combined sources
Beta strandi673 – 6786Combined sources
Beta strandi685 – 6884Combined sources
Beta strandi690 – 6934Combined sources
Beta strandi695 – 70713Combined sources
Helixi728 – 7303Combined sources
Helixi732 – 7343Combined sources
Beta strandi745 – 75410Combined sources
Beta strandi759 – 7624Combined sources
Beta strandi766 – 77510Combined sources
Beta strandi780 – 7856Combined sources
Beta strandi791 – 7944Combined sources
Turni796 – 7983Combined sources
Beta strandi801 – 8044Combined sources
Helixi810 – 8134Combined sources
Beta strandi819 – 8235Combined sources
Beta strandi825 – 8273Combined sources
Beta strandi832 – 84211Combined sources
Beta strandi844 – 8463Combined sources
Beta strandi851 – 8544Combined sources
Beta strandi858 – 8614Combined sources
Beta strandi866 – 88217Combined sources
Turni886 – 8883Combined sources
Beta strandi894 – 91118Combined sources
Helixi912 – 9143Combined sources
Beta strandi919 – 9268Combined sources
Beta strandi928 – 93811Combined sources
Beta strandi946 – 9505Combined sources
Beta strandi959 – 9657Combined sources
Beta strandi976 – 9805Combined sources
Beta strandi983 – 9875Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H09X-ray1.75A/B26-1014[»]
ProteinModelPortaliP44969.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44969.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini26 – 332307Peptidase S6PROSITE-ProRule annotationAdd
BLAST
Domaini1442 – 1694253AutotransporterPROSITE-ProRule annotationAdd
BLAST

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108BMY. Bacteria.
COG3468. LUCA.
KOiK01347.
OMAiAAPQDYM.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
IPR004899. Pertactin_central.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGRFSVG
60 70 80 90 100
ATNVEVRDKN NHSLGNVLPN GIPMIDFSVV DVDKRIATLI NPQYVVGVKH
110 120 130 140 150
VSNGVSELHF GNLNGNMNNG NAKSHRDVSS EENRYFSVEK NEYPTKLNGK
160 170 180 190 200
AVTTEDQTQK RREDYYMPRL DKFVTEVAPI EASTASSDAG TYNDQNKYPA
210 220 230 240 250
FVRLGSGSQF IYKKGDNYSL ILNNHEVGGN NLKLVGDAYT YGIAGTPYKV
260 270 280 290 300
NHENNGLIGF GNSKEEHSDP KGILSQDPLT NYAVLGDSGS PLFVYDREKG
310 320 330 340 350
KWLFLGSYDF WAGYNKKSWQ EWNIYKPEFA KTVLDKDTAG SLTGSNTQYN
360 370 380 390 400
WNPTGKTSVI SNGSESLNVD LFDSSQDTDS KKNNHGKSVT LRGSGTLTLN
410 420 430 440 450
NNIDQGAGGL FFEGDYEVKG TSDSTTWKGA GVSVADGKTV TWKVHNPKSD
460 470 480 490 500
RLAKIGKGTL IVEGKGENKG SLKVGDGTVI LKQQADANNK VKAFSQVGIV
510 520 530 540 550
SGRSTVVLND DKQVDPNSIY FGFRGGRLDA NGNNLTFEHI RNIDDGARLV
560 570 580 590 600
NHNTSKTSTV TITGESLITD PNTITPYNID APDEDNPYAF RRIKDGGQLY
610 620 630 640 650
LNLENYTYYA LRKGASTRSE LPKNSGESNE NWLYMGKTSD EAKRNVMNHI
660 670 680 690 700
NNERMNGFNG YFGEEEGKNN GNLNVTFKGK SEQNRFLLTG GTNLNGDLKV
710 720 730 740 750
EKGTLFLSGR PTPHARDIAG ISSTKKDQHF AENNEVVVED DWINRNFKAT
760 770 780 790 800
NINVTNNATL YSGRNVANIT SNITASDNAK VHIGYKAGDT VCVRSDYTGY
810 820 830 840 850
VTCTTDKLSD KALNSFNATN VSGNVNLSGN ANFVLGKANL FGTISGTGNS
860 870 880 890 900
QVRLTENSHW HLTGDSNVNQ LNLDKGHIHL NAQNDANKVT TYNTLTVNSL
910 920 930 940 950
SGNGSFYYLT DLSNKQGDKV VVTKSATGNF TLQVADKTGE PTKNELTLFD
960 970 980 990 1000
ASNATRNNLN VSLVGNTVDL GAWKYKLRNV NGRYDLYNPE VEKRNQTVDT
1010 1020 1030 1040 1050
TNITTPNNIQ ADVPSVPSNN EEIARVETPV PPPAPATPSE TTETVAENSK
1060 1070 1080 1090 1100
QESKTVEKNE QDATETTAQN GEVAEEAKPS VKANTQTNEV AQSGSETEET
1110 1120 1130 1140 1150
QTTEIKETAK VEKEEKAKVE KDEIQEAPQM ASETSPKQAK PAPKEVSTDT
1160 1170 1180 1190 1200
KVEETQVQAQ PQTQSTTVAA AEATSPNSKP AEETQPSEKT NAEPVTPVVS
1210 1220 1230 1240 1250
KNQTENTTDQ PTEREKTAKV ETEKTQEPPQ VASQASPKQE QSETVQPQAV
1260 1270 1280 1290 1300
LESENVPTVN NAEEVQAQLQ TQTSATVSTK QPAPENSINT GSATAITETA
1310 1320 1330 1340 1350
EKSDKPQTET AASTEDASQH KANTVADNSV ANNSESSDPK SRRRRSISQP
1360 1370 1380 1390 1400
QETSAEETTA ASTDETTIAD NSKRSKPNRR SRRSVRSEPT VTNGSDRSTV
1410 1420 1430 1440 1450
ALRDLTSTNT NAVISDAMAK AQFVALNVGK AVSQHISQLE MNNEGQYNVW
1460 1470 1480 1490 1500
VSNTSMNENY SSSQYRRFSS KSTQTQLGWD QTISNNVQLG GVFTYVRNSN
1510 1520 1530 1540 1550
NFDKASSKNT LAQVNFYSKY YADNHWYLGI DLGYGKFQSN LKTNHNAKFA
1560 1570 1580 1590 1600
RHTAQFGLTA GKAFNLGNFG ITPIVGVRYS YLSNANFALA KDRIKVNPIS
1610 1620 1630 1640 1650
VKTAFAQVDL SYTYHLGEFS VTPILSARYD TNQGSGKINV NQYDFAYNVE
1660 1670 1680 1690
NQQQYNAGLK LKYHNVKLSL IGGLTKAKQA EKQKTAELKL SFSF
Length:1,694
Mass (Da):185,539
Last modified:November 1, 1995 - v1
Checksum:iC52427013F93178C
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti253 – 2542EN → GV in CAB56789 (Ref. 1) Curated
Sequence conflicti272 – 2721G → A in CAB56789 (Ref. 1) Curated
Sequence conflicti464 – 4641G → E in CAB56789 (Ref. 1) Curated
Sequence conflicti866 – 8661S → T in CAB56789 (Ref. 1) Curated
Sequence conflicti1036 – 10361A → D in CAB56789 (Ref. 1) Curated
Sequence conflicti1074 – 10741A → G in CAB56789 (Ref. 1) Curated
Sequence conflicti1421 – 14211A → G in CAB56789 (Ref. 1) Curated
Sequence conflicti1545 – 15451H → T in CAB56789 (Ref. 1) Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59800 Genomic DNA. Translation: CAB56789.1.
L42023 Genomic DNA. Translation: AAC22651.1.
PIRiH64106.
RefSeqiNP_439153.1. NC_000907.1.
WP_005693332.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22651; AAC22651; HI_0990.
GeneIDi950762.
KEGGihin:HI0990.
PATRICi20190641. VBIHaeInf48452_1033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59800 Genomic DNA. Translation: CAB56789.1.
L42023 Genomic DNA. Translation: AAC22651.1.
PIRiH64106.
RefSeqiNP_439153.1. NC_000907.1.
WP_005693332.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
3H09X-ray1.75A/B26-1014[»]
ProteinModelPortaliP44969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22651; AAC22651; HI_0990.
GeneIDi950762.
KEGGihin:HI0990.
PATRICi20190641. VBIHaeInf48452_1033.

Phylogenomic databases

eggNOGiENOG4108BMY. Bacteria.
COG3468. LUCA.
KOiK01347.
OMAiAAPQDYM.

Enzyme and pathway databases

BRENDAi3.4.21.72. 2529.

Miscellaneous databases

EvolutionaryTraceiP44969.
PMAP-CutDBP44969.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
IPR004899. Pertactin_central.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIGA0_HAEIN
AccessioniPrimary (citable) accession number: P44969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 123 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.