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Protein

Immunoglobulin A1 protease autotransporter

Gene

iga

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Virulence factor; cleaves host immunoglobulin A producing intact Fc and Fab fragments.

Catalytic activityi

Cleavage of immunoglobulin A molecules at certain Pro-|-Xaa bonds in the hinge region. No small molecule substrates are known.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei288Curated1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Keywords - Biological processi

Virulence

Enzyme and pathway databases

BRENDAi3.4.21.72. 2529.

Names & Taxonomyi

Protein namesi
Recommended name:
Immunoglobulin A1 protease autotransporter (EC:3.4.21.72)
Cleaved into the following 2 chains:
Immunoglobulin A1 protease
Short name:
IGA1 protease
Alternative name(s):
Helper peptide
Gene namesi
Name:iga
Synonyms:iga1
Ordered Locus Names:HI_0990
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

Immunoglobulin A1 protease translocator :

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell outer membrane, Membrane, Periplasm, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 25Sequence analysisAdd BLAST25
ChainiPRO_000038759826 – 1694Immunoglobulin A1 protease autotransporterAdd BLAST1669
ChainiPRO_000002695826 – 1014Immunoglobulin A1 proteaseAdd BLAST989
ChainiPRO_00000269591015 – 1694Immunoglobulin A1 protease translocatorSequence analysisAdd BLAST680

Keywords - PTMi

Zymogen

Miscellaneous databases

PMAP-CutDBP44969.

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0990.

Structurei

Secondary structure

11694
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi30 – 32Combined sources3
Helixi34 – 42Combined sources9
Beta strandi55 – 57Combined sources3
Beta strandi63 – 69Combined sources7
Helixi77 – 79Combined sources3
Turni82 – 84Combined sources3
Beta strandi88 – 91Combined sources4
Beta strandi94 – 97Combined sources4
Beta strandi106 – 109Combined sources4
Beta strandi111 – 113Combined sources3
Helixi130 – 133Combined sources4
Beta strandi134 – 139Combined sources6
Beta strandi148 – 150Combined sources3
Helixi154 – 162Combined sources9
Beta strandi169 – 172Combined sources4
Helixi191 – 193Combined sources3
Turni195 – 197Combined sources3
Beta strandi200 – 205Combined sources6
Beta strandi207 – 218Combined sources12
Beta strandi229 – 236Combined sources8
Beta strandi243 – 246Combined sources4
Beta strandi248 – 251Combined sources4
Beta strandi257 – 261Combined sources5
Helixi273 – 275Combined sources3
Beta strandi291 – 296Combined sources6
Turni297 – 300Combined sources4
Beta strandi301 – 311Combined sources11
Helixi313 – 316Combined sources4
Beta strandi319 – 324Combined sources6
Helixi327 – 337Combined sources11
Beta strandi338 – 354Combined sources17
Beta strandi357 – 362Combined sources6
Beta strandi365 – 368Combined sources4
Beta strandi376 – 378Combined sources3
Helixi379 – 384Combined sources6
Beta strandi388 – 401Combined sources14
Beta strandi410 – 412Combined sources3
Beta strandi414 – 422Combined sources9
Beta strandi426 – 430Combined sources5
Beta strandi432 – 434Combined sources3
Beta strandi439 – 442Combined sources4
Beta strandi451 – 462Combined sources12
Beta strandi465 – 467Combined sources3
Beta strandi471 – 474Combined sources4
Beta strandi476 – 481Combined sources6
Beta strandi491 – 493Combined sources3
Beta strandi497 – 499Combined sources3
Beta strandi504 – 510Combined sources7
Helixi516 – 518Combined sources3
Beta strandi519 – 521Combined sources3
Beta strandi526 – 529Combined sources4
Beta strandi535 – 538Combined sources4
Beta strandi541 – 544Combined sources4
Beta strandi547 – 550Combined sources4
Beta strandi558 – 562Combined sources5
Helixi571 – 573Combined sources3
Beta strandi577 – 579Combined sources3
Beta strandi600 – 602Combined sources3
Turni603 – 606Combined sources4
Beta strandi607 – 611Combined sources5
Beta strandi630 – 638Combined sources9
Helixi639 – 653Combined sources15
Beta strandi660 – 663Combined sources4
Beta strandi673 – 678Combined sources6
Beta strandi685 – 688Combined sources4
Beta strandi690 – 693Combined sources4
Beta strandi695 – 707Combined sources13
Helixi728 – 730Combined sources3
Helixi732 – 734Combined sources3
Beta strandi745 – 754Combined sources10
Beta strandi759 – 762Combined sources4
Beta strandi766 – 775Combined sources10
Beta strandi780 – 785Combined sources6
Beta strandi791 – 794Combined sources4
Turni796 – 798Combined sources3
Beta strandi801 – 804Combined sources4
Helixi810 – 813Combined sources4
Beta strandi819 – 823Combined sources5
Beta strandi825 – 827Combined sources3
Beta strandi832 – 842Combined sources11
Beta strandi844 – 846Combined sources3
Beta strandi851 – 854Combined sources4
Beta strandi858 – 861Combined sources4
Beta strandi866 – 882Combined sources17
Turni886 – 888Combined sources3
Beta strandi894 – 911Combined sources18
Helixi912 – 914Combined sources3
Beta strandi919 – 926Combined sources8
Beta strandi928 – 938Combined sources11
Beta strandi946 – 950Combined sources5
Beta strandi959 – 965Combined sources7
Beta strandi976 – 980Combined sources5
Beta strandi983 – 987Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H09X-ray1.75A/B26-1014[»]
ProteinModelPortaliP44969.
SMRiP44969.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44969.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini26 – 332Peptidase S6PROSITE-ProRule annotationAdd BLAST307
Domaini1442 – 1694AutotransporterPROSITE-ProRule annotationAdd BLAST253

Domaini

The signal peptide, cleaved at the inner membrane, guides the autotransporter protein to the periplasmic space. Then, insertion of the C-terminal translocator domain in the outer membrane forms a hydrophilic pore for the translocation of the passenger domain to the bacterial cell surface, with subsequent cleavage (By similarity).By similarity

Sequence similaritiesi

Contains 1 autotransporter (TC 1.B.12) domain. [View classification]PROSITE-ProRule annotation
Contains 1 peptidase S6 domain.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane beta strand

Phylogenomic databases

eggNOGiENOG4108BMY. Bacteria.
COG3468. LUCA.
KOiK01347.
OMAiAAPQDYM.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
IPR004899. Pertactin_central.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44969-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLNKKFKLNF IALTVAYALT PYTEAALVRD DVDYQIFRDF AENKGRFSVG
60 70 80 90 100
ATNVEVRDKN NHSLGNVLPN GIPMIDFSVV DVDKRIATLI NPQYVVGVKH
110 120 130 140 150
VSNGVSELHF GNLNGNMNNG NAKSHRDVSS EENRYFSVEK NEYPTKLNGK
160 170 180 190 200
AVTTEDQTQK RREDYYMPRL DKFVTEVAPI EASTASSDAG TYNDQNKYPA
210 220 230 240 250
FVRLGSGSQF IYKKGDNYSL ILNNHEVGGN NLKLVGDAYT YGIAGTPYKV
260 270 280 290 300
NHENNGLIGF GNSKEEHSDP KGILSQDPLT NYAVLGDSGS PLFVYDREKG
310 320 330 340 350
KWLFLGSYDF WAGYNKKSWQ EWNIYKPEFA KTVLDKDTAG SLTGSNTQYN
360 370 380 390 400
WNPTGKTSVI SNGSESLNVD LFDSSQDTDS KKNNHGKSVT LRGSGTLTLN
410 420 430 440 450
NNIDQGAGGL FFEGDYEVKG TSDSTTWKGA GVSVADGKTV TWKVHNPKSD
460 470 480 490 500
RLAKIGKGTL IVEGKGENKG SLKVGDGTVI LKQQADANNK VKAFSQVGIV
510 520 530 540 550
SGRSTVVLND DKQVDPNSIY FGFRGGRLDA NGNNLTFEHI RNIDDGARLV
560 570 580 590 600
NHNTSKTSTV TITGESLITD PNTITPYNID APDEDNPYAF RRIKDGGQLY
610 620 630 640 650
LNLENYTYYA LRKGASTRSE LPKNSGESNE NWLYMGKTSD EAKRNVMNHI
660 670 680 690 700
NNERMNGFNG YFGEEEGKNN GNLNVTFKGK SEQNRFLLTG GTNLNGDLKV
710 720 730 740 750
EKGTLFLSGR PTPHARDIAG ISSTKKDQHF AENNEVVVED DWINRNFKAT
760 770 780 790 800
NINVTNNATL YSGRNVANIT SNITASDNAK VHIGYKAGDT VCVRSDYTGY
810 820 830 840 850
VTCTTDKLSD KALNSFNATN VSGNVNLSGN ANFVLGKANL FGTISGTGNS
860 870 880 890 900
QVRLTENSHW HLTGDSNVNQ LNLDKGHIHL NAQNDANKVT TYNTLTVNSL
910 920 930 940 950
SGNGSFYYLT DLSNKQGDKV VVTKSATGNF TLQVADKTGE PTKNELTLFD
960 970 980 990 1000
ASNATRNNLN VSLVGNTVDL GAWKYKLRNV NGRYDLYNPE VEKRNQTVDT
1010 1020 1030 1040 1050
TNITTPNNIQ ADVPSVPSNN EEIARVETPV PPPAPATPSE TTETVAENSK
1060 1070 1080 1090 1100
QESKTVEKNE QDATETTAQN GEVAEEAKPS VKANTQTNEV AQSGSETEET
1110 1120 1130 1140 1150
QTTEIKETAK VEKEEKAKVE KDEIQEAPQM ASETSPKQAK PAPKEVSTDT
1160 1170 1180 1190 1200
KVEETQVQAQ PQTQSTTVAA AEATSPNSKP AEETQPSEKT NAEPVTPVVS
1210 1220 1230 1240 1250
KNQTENTTDQ PTEREKTAKV ETEKTQEPPQ VASQASPKQE QSETVQPQAV
1260 1270 1280 1290 1300
LESENVPTVN NAEEVQAQLQ TQTSATVSTK QPAPENSINT GSATAITETA
1310 1320 1330 1340 1350
EKSDKPQTET AASTEDASQH KANTVADNSV ANNSESSDPK SRRRRSISQP
1360 1370 1380 1390 1400
QETSAEETTA ASTDETTIAD NSKRSKPNRR SRRSVRSEPT VTNGSDRSTV
1410 1420 1430 1440 1450
ALRDLTSTNT NAVISDAMAK AQFVALNVGK AVSQHISQLE MNNEGQYNVW
1460 1470 1480 1490 1500
VSNTSMNENY SSSQYRRFSS KSTQTQLGWD QTISNNVQLG GVFTYVRNSN
1510 1520 1530 1540 1550
NFDKASSKNT LAQVNFYSKY YADNHWYLGI DLGYGKFQSN LKTNHNAKFA
1560 1570 1580 1590 1600
RHTAQFGLTA GKAFNLGNFG ITPIVGVRYS YLSNANFALA KDRIKVNPIS
1610 1620 1630 1640 1650
VKTAFAQVDL SYTYHLGEFS VTPILSARYD TNQGSGKINV NQYDFAYNVE
1660 1670 1680 1690
NQQQYNAGLK LKYHNVKLSL IGGLTKAKQA EKQKTAELKL SFSF
Length:1,694
Mass (Da):185,539
Last modified:November 1, 1995 - v1
Checksum:iC52427013F93178C
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti253 – 254EN → GV in CAB56789 (Ref. 1) Curated2
Sequence conflicti272G → A in CAB56789 (Ref. 1) Curated1
Sequence conflicti464G → E in CAB56789 (Ref. 1) Curated1
Sequence conflicti866S → T in CAB56789 (Ref. 1) Curated1
Sequence conflicti1036A → D in CAB56789 (Ref. 1) Curated1
Sequence conflicti1074A → G in CAB56789 (Ref. 1) Curated1
Sequence conflicti1421A → G in CAB56789 (Ref. 1) Curated1
Sequence conflicti1545H → T in CAB56789 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59800 Genomic DNA. Translation: CAB56789.1.
L42023 Genomic DNA. Translation: AAC22651.1.
PIRiH64106.
RefSeqiNP_439153.1. NC_000907.1.
WP_005693332.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22651; AAC22651; HI_0990.
GeneIDi950762.
KEGGihin:HI0990.
PATRICi20190641. VBIHaeInf48452_1033.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X59800 Genomic DNA. Translation: CAB56789.1.
L42023 Genomic DNA. Translation: AAC22651.1.
PIRiH64106.
RefSeqiNP_439153.1. NC_000907.1.
WP_005693332.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3H09X-ray1.75A/B26-1014[»]
ProteinModelPortaliP44969.
SMRiP44969.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0990.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22651; AAC22651; HI_0990.
GeneIDi950762.
KEGGihin:HI0990.
PATRICi20190641. VBIHaeInf48452_1033.

Phylogenomic databases

eggNOGiENOG4108BMY. Bacteria.
COG3468. LUCA.
KOiK01347.
OMAiAAPQDYM.

Enzyme and pathway databases

BRENDAi3.4.21.72. 2529.

Miscellaneous databases

EvolutionaryTraceiP44969.
PMAP-CutDBP44969.

Family and domain databases

Gene3Di2.160.20.20. 1 hit.
2.40.128.130. 1 hit.
InterProiIPR005546. Autotransporte_beta.
IPR012332. P22_tailspike_C-like.
IPR011050. Pectin_lyase_fold/virulence.
IPR000710. Peptidase_S6.
IPR030396. Peptidase_S6_dom.
IPR004899. Pertactin_central.
[Graphical view]
PfamiPF03797. Autotransporter. 1 hit.
PF02395. Peptidase_S6. 1 hit.
PF03212. Pertactin. 1 hit.
[Graphical view]
PRINTSiPR00921. IGASERPTASE.
SMARTiSM00869. Autotransporter. 1 hit.
[Graphical view]
SUPFAMiSSF103515. SSF103515. 1 hit.
SSF51126. SSF51126. 3 hits.
PROSITEiPS51208. AUTOTRANSPORTER. 1 hit.
PS51691. PEPTIDASE_S6. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIGA0_HAEIN
AccessioniPrimary (citable) accession number: P44969
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. Peptidase families
    Classification of peptidase families and list of entries
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.