P44961 (MENC_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
o-succinylbenzoate synthase
Short name=OSB synthase
Short name=OSBS
EC=4.2.1.113

Alternative name(s):
4-(2'-carboxyphenyl)-4-oxybutyric acid synthase
o-succinylbenzoic acid synthase

Gene names

Name:	menC
Ordered Locus Names:	HI_0969

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	329 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts SHCHC to OSB By similarity.
Catalytic activity	(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H2O. HAMAP-Rule MF_00470
Cofactor	Magnesium By similarity.
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 4/8. HAMAP-Rule MF_00470
Sequence similarities	Belongs to the mandelate racemase/muconate lactonizing enzyme family. MenC type 1 subfamily.
Sequence caution	The sequence AAC22626.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Lyase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	menaquinone biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular_function	hydro-lyase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 329	329	o-succinylbenzoate synthase HAMAP-Rule MF_00470		PRO_0000171272
Sites
	Active site	140	1	Proton donor By similarity
	Active site	242	1	Proton acceptor By similarity
	Metal binding	168	1	Magnesium By similarity
	Metal binding	197	1	Magnesium By similarity
	Metal binding	220	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44961 [UniParc]. Last modified November 2, 2001. Version 2. Checksum: 26353A1B50D5FF60	FASTA	329	36,970
	10 20 30 40 50 60 MAEKSFNLYR YSIPVDSQLI LRDRFLKRRE GLIVRVSCSR DGWGEIAPLP GFSEETLDQA 70 80 90 100 110 120 QEQAIEWLTT WCNASCDAPR VPLDGTYPSV AFGISCAMDE MKGYLQAEGN YHTAPLCYGD 130 140 150 160 170 180 PDELYAKLAS MEGEKVAKMK VGIYEANRDG LIADMFLEAI PDLQLRLDAN RHWSLEKALQ 190 200 210 220 230 240 FAAKVKLQHR KRIQFLEEPC KTQALSREFA VQTDIAIAWD ESVREPNFCL EKEPHLSAVV 250 260 270 280 290 300 IKPTLIGSIQ RCTELINQAH SLGLKAVISS SIESSLGLSQ LARIAQQYTP NVTPGLDTLD 310 320 LMEYQVLRAW PSSDLPIVDL ESEFITKII

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22626.1. Different initiation.
PIR	C64105.
RefSeq	NP_439130.2. NC_000907.1.
3D structure databases
ProteinModelPortal	P44961.
SMR	P44961. Positions 4-322.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0969.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22626; AAC22626; HI_0969.
GeneID	949968.
KEGG	hin:HI0969.
PATRIC	20190595. VBIHaeInf48452_1010.
Phylogenomic databases
eggNOG	COG1441.
KO	K02549.
OMA	YEANRDG.
ProtClustDB	PRK05105.
Enzyme and pathway databases
UniPathway	UPA00079; UER00165.
Family and domain databases
HAMAP	MF_00470. MenC_1.
InterPro	IPR013342. Mandelate_racemase_C. IPR010196. OSB_synthase. [Graphical view]
Pfam	PF01188. MR_MLE. 1 hit. [Graphical view]
SMART	SM00922. MR_MLE. 1 hit. [Graphical view]
TIGRFAMs	TIGR01927. menC_gamma/gm+. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MENC_HAEIN

Accession

Primary (citable) accession number: P44961

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 2, 2001
Last modified:	May 1, 2013
This is version 88 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program