P44957 (RIBF_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Riboflavin biosynthesis protein RibF

Including the following 2 domains:

1. Riboflavin kinase
   EC=2.7.1.26
   Alternative name(s):
   Flavokinase
2. FMN adenylyltransferase
   EC=2.7.7.2
   Alternative name(s):
   FAD pyrophosphorylase
   FAD synthase

Gene names

Name:	ribF
Ordered Locus Names:	HI_0963

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + riboflavin = ADP + FMN. ATP + FMN = diphosphate + FAD.
Pathway	Cofactor biosynthesis; FAD biosynthesis; FAD from FMN: step 1/1. Cofactor biosynthesis; FMN biosynthesis; FMN from riboflavin (ATP route): step 1/1.
Sequence similarities	Belongs to the RibF family.
Sequence caution	The sequence AAC22622.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Ontologies

Keywords
Ligand	ATP-binding FAD FMN Nucleotide-binding
Molecular function	Kinase Nucleotidyltransferase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological_process	FAD biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway FMN biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway riboflavin biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW FMN adenylyltransferase activity Inferred from electronic annotation. Source: EC riboflavin kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	Riboflavin biosynthesis protein RibF		PRO_0000194140

Sequences

	Sequence		Length	Mass (Da)
	P44957 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5DA4EA0F5492923E	FASTA	308	35,121
	10 20 30 40 50 60 MQLIRGLHNA NRVLQGCALT IGNFDGVHLG HQTVLRHLRQ KADELNLPMA VLLFESQPRE 70 80 90 100 110 120 YFMGKNAPAR LMRLRDKIYY LEKAKVDVVI VAKFDRTFAE QLADVFIEQT LVNHLHVKFL 130 140 150 160 170 180 SIGDDFKFGS KRQGNFAMLQ AASKRFGFIV EDNRSFCLDA QRISSTAIRE ALANDDLQLA 190 200 210 220 230 240 ENLLGKPYRI FGRVIHGNKL GRTIGFPTAN IRLHRQVNPI KGVYAVKVRL KSGEIFNGVA 250 260 270 280 290 300 NMGKRPTING LMQLLEVHLF DFSQNIYGQM VEVEFCHKIR NEIKFPSFDD LKVQIEKDVE TAKAFFNS

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22622.1. Different initiation.
PIR	H64162.
RefSeq	NP_439124.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44957.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0963.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22622; AAC22622; HI_0963.
GeneID	949965.
KEGG	hin:HI0963.
PATRIC	20190585. VBIHaeInf48452_1005.
Phylogenomic databases
eggNOG	COG0196.
KO	K11753.
OMA	FHFGRGR.
ProtClustDB	PRK05627.
Enzyme and pathway databases
UniPathway	UPA00276; UER00406. UPA00277; UER00407.
Family and domain databases
Gene3D	2.40.30.30. 1 hit. 3.40.50.620. 1 hit.
InterPro	IPR004821. Cyt_trans-like. IPR015864. FAD_synthase. IPR023468. Riboflavin_kinase. IPR002606. Riboflavin_kinase_bac. IPR015865. Riboflavin_kinase_bac/euk. IPR023465. Riboflavin_kinase_domain. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
PANTHER	PTHR22749. PTHR22749. 1 hit.
Pfam	PF06574. FAD_syn. 1 hit. PF01687. Flavokinase. 1 hit. [Graphical view]
PIRSF	PIRSF004491. FAD_Synth. 1 hit.
SMART	SM00904. Flavokinase. 1 hit. [Graphical view]
SUPFAM	SSF82114. Riboflavin_kinase. 1 hit.
TIGRFAMs	TIGR00125. cyt_tran_rel. 1 hit. TIGR00083. ribF. 1 hit.
ProtoNet	Search...

Entry information

Entry name

RIBF_HAEIN

Accession

Primary (citable) accession number: P44957

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 82 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program