P44956 (Y961_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
HIT-like protein HI_0961
EC=3.-.-.-

Alternative name(s):
Purine nucleoside phosphoramidase

Gene names

Ordered Locus Names:

HI_0961

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	116 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Hydrolyzes purine nucleotide phosphoramidates, including adenosine 5'monophosphoramidate (AMP-NH2), adenosine 5'monophosphomorpholidate (AMP-morpholidate), guanosine 5'monophosphomorpholidate (GMP-morpholidate) and tryptamine 5'guanosine monophosphate (TpGd). Hydrolyzes lysyl-AMP (AMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) generated by lysine tRNA ligase and lysyl-GMP (GMP-N-epsilon-(N-alpha-acetyl lysine methyl ester)) By similarity.
Subunit structure	Homodimer By similarity.
Sequence similarities	Belongs to the HINT family. Contains 1 HIT domain.
Sequence caution	The sequence AAC22621.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Ligand	Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	metabolic process Inferred from electronic annotation. Source: GOC
Molecular_function	hydrolase activity Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 116	116	HIT-like protein HI_0961		PRO_0000109833
Regions
	Domain	6 – 115	110	HIT
	Nucleotide binding	30 – 32	3	Purine nucleotide phosphoramidate By similarity
	Nucleotide binding	96 – 97	2	Purine nucleotide phosphoramidate By similarity
	Nucleotide binding	101 – 103	3	Purine nucleotide phosphoramidate By similarity
	Motif	99 – 103	5	Histidine triad motif
Sites
	Active site	101	1	Tele-AMP-histidine intermediate By similarity
	Binding site	88	1	Purine nucleotide phosphoramidate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44956 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 904B9A3211757F12	FASTA	116	12,887
	10 20 30 40 50 60 MAEETIFSKI IRKEIPANIV YQDELVTAFR DISPQAKTHI LIIPNKVIPT VNDVTEQDEV 70 80 90 100 110 ALGRLFSVAA KLAKEEGVAE DGYRLIVNCN KHGGQEVFHL HMHLVGGEPL GRMLAK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22621.1. Different initiation.
PIR	G64162.
RefSeq	NP_439122.2. NC_000907.1.
3D structure databases
ProteinModelPortal	P44956.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0961.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22621; AAC22621; HI_0961.
GeneID	950594.
KEGG	hin:HI0961.
PATRIC	20190581. VBIHaeInf48452_1003.
Phylogenomic databases
eggNOG	COG0537.
OMA	NCNRHGG.
ProtClustDB	CLSK2462468.
Family and domain databases
Gene3D	3.30.428.10. 1 hit.
InterPro	IPR019808. Histidine_triad_CS. IPR001310. Histidine_triad_HIT. IPR011146. HIT-like. [Graphical view]
PANTHER	PTHR23089. PTHR23089. 1 hit.
Pfam	PF01230. HIT. 1 hit. [Graphical view]
PRINTS	PR00332. HISTRIAD.
SUPFAM	SSF54197. His_triad-like_motif. 1 hit.
PROSITE	PS00892. HIT_1. 1 hit. PS51084. HIT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Y961_HAEIN

Accession

Primary (citable) accession number: P44956

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 76 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program