ID   NRFF_HAEIN              Reviewed;         384 AA.
AC   P44942;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   06-JUN-2002, sequence version 2.
DT   27-MAR-2024, entry version 141.
DE   RecName: Full=Formate-dependent nitrite reductase complex bifunctional subunit NrfFG;
DE   Flags: Precursor;
GN   Name=nrfF; OrderedLocusNames=HI_0934;
OS   Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Pasteurellales;
OC   Pasteurellaceae; Haemophilus.
OX   NCBI_TaxID=71421;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 51907 / DSM 11121 / KW20 / Rd;
RX   PubMed=7542800; DOI=10.1126/science.7542800;
RA   Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F.,
RA   Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M.,
RA   McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D.,
RA   Scott J.D., Shirley R., Liu L.-I., Glodek A., Kelley J.M., Weidman J.F.,
RA   Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R.,
RA   Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D.,
RA   Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A.,
RA   Small K.V., Fraser C.M., Smith H.O., Venter J.C.;
RT   "Whole-genome random sequencing and assembly of Haemophilus influenzae
RT   Rd.";
RL   Science 269:496-512(1995).
CC   -!- FUNCTION: Possible subunit of a heme lyase. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Periplasm {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the CcmH/CycL/Ccl2/NrfF family. {ECO:0000305}.
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DR   EMBL; L42023; AAC22592.1; -; Genomic_DNA.
DR   PIR; H64161; H64161.
DR   RefSeq; NP_439094.1; NC_000907.1.
DR   AlphaFoldDB; P44942; -.
DR   SMR; P44942; -.
DR   STRING; 71421.HI_0934; -.
DR   EnsemblBacteria; AAC22592; AAC22592; HI_0934.
DR   KEGG; hin:HI_0934; -.
DR   PATRIC; fig|71421.8.peg.975; -.
DR   eggNOG; COG3088; Bacteria.
DR   eggNOG; COG4235; Bacteria.
DR   HOGENOM; CLU_036074_0_1_6; -.
DR   OrthoDB; 9776053at2; -.
DR   PhylomeDB; P44942; -.
DR   BioCyc; HINF71421:G1GJ1-974-MONOMER; -.
DR   Proteomes; UP000000579; Chromosome.
DR   GO; GO:0042597; C:periplasmic space; IEA:UniProtKB-SubCell.
DR   GO; GO:0005886; C:plasma membrane; IBA:GO_Central.
DR   GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR   CDD; cd16378; CcmH_N; 1.
DR   Gene3D; 1.10.8.640; Cytochrome C biogenesis protein; 1.
DR   Gene3D; 1.25.40.10; Tetratricopeptide repeat domain; 1.
DR   InterPro; IPR005616; CcmH/CycL/Ccl2/NrfF.
DR   InterPro; IPR038297; CcmH/CycL/NrfF/Ccl2_sf.
DR   InterPro; IPR017565; For-dep_Cytc_NO2Rdtase_NrfF.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   NCBIfam; TIGR03147; cyt_nit_nrfF; 1.
DR   PANTHER; PTHR47870; CYTOCHROME C-TYPE BIOGENESIS PROTEIN CCMH; 1.
DR   PANTHER; PTHR47870:SF2; FORMATE-DEPENDENT NITRITE REDUCTASE COMPLEX SUBUNIT NRFF; 1.
DR   Pfam; PF03918; CcmH; 1.
DR   SMART; SM00028; TPR; 1.
DR   SUPFAM; SSF48452; TPR-like; 1.
DR   PROSITE; PS50005; TPR; 1.
DR   PROSITE; PS50293; TPR_REGION; 1.
PE   3: Inferred from homology;
KW   Heme; Iron; Metal-binding; Periplasm; Reference proteome; Repeat; Signal;
KW   TPR repeat.
FT   SIGNAL          1..35
FT                   /evidence="ECO:0000255"
FT   CHAIN           36..384
FT                   /note="Formate-dependent nitrite reductase complex
FT                   bifunctional subunit NrfFG"
FT                   /id="PRO_0000006616"
FT   REPEAT          221..254
FT                   /note="TPR 1"
FT   REPEAT          255..288
FT                   /note="TPR 2"
FT   REGION          36..144
FT                   /note="NrfF"
FT   REGION          180..384
FT                   /note="NrfG"
FT   BINDING         61
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255"
FT   BINDING         64
FT                   /ligand="heme"
FT                   /ligand_id="ChEBI:CHEBI:30413"
FT                   /note="covalent"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   384 AA;  43780 MW;  0321F4A7704964C4 CRC64;
     MKSILDIFTR GFIQVQKTVF FATALLFAFS LFTQAEMVDT YQFQNQDDRT RAVELAKSLR
     CPQCQNQNLV ESNSPIAYDL RLEVYKMVDE GKSNQQIIDK MTARFGDFVN YKPPFKWNTA
     LLWLLPVALL ILAAVLLYFS NRKKQFSEKV VAQQLENDEI ISLPSTFGSS PRKQGEPSKL
     SKGKVNSKIY FVLFTLLIAI PATYYFSLDR FSRVQQGEQS MIEQHNQNVE MNDEHKNENV
     IEKLQNKLRT DPNNAETWLQ LGEAYVQNNE FDSALVAYSN AEKLSGSKPN ILGLAATALY
     YQAGQQMTQK VEQLLNEALA KDKNEVSSLS LLATIALENR QYQQAGMYLQ QLLDSGNAAV
     DRRSVIQRMK MLDFLQRGEK GQNP
//