P44942 (NRFF_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Formate-dependent nitrite reductase complex bifunctional subunit NrfFG

Gene names

Name:	nrfF
Ordered Locus Names:	HI_0934

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	384 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Possible subunit of a heme lyase By similarity.
Subcellular location	Periplasm Probable.
Sequence similarities	Belongs to the CcmH/CycL/Ccl2/NrfF family. Contains 2 TPR repeats.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Repeat Signal TPR repeat
Ligand	Heme Iron Metal-binding
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 35	35	Potential
	Chain	36 – 384	349	Formate-dependent nitrite reductase complex bifunctional subunit NrfFG		PRO_0000006616
Regions
	Repeat	221 – 254	34	TPR 1
	Repeat	255 – 288	34	TPR 2
	Region	36 – 144	109	NrfF
	Region	180 – 384	205	NrfG
Sites
	Binding site	61	1	Heme (covalent) Potential
	Binding site	64	1	Heme (covalent) Potential

Sequences

	Sequence		Length	Mass (Da)
	P44942 [UniParc]. Last modified June 6, 2002. Version 2. Checksum: 0321F4A7704964C4	FASTA	384	43,780
	10 20 30 40 50 60 MKSILDIFTR GFIQVQKTVF FATALLFAFS LFTQAEMVDT YQFQNQDDRT RAVELAKSLR 70 80 90 100 110 120 CPQCQNQNLV ESNSPIAYDL RLEVYKMVDE GKSNQQIIDK MTARFGDFVN YKPPFKWNTA 130 140 150 160 170 180 LLWLLPVALL ILAAVLLYFS NRKKQFSEKV VAQQLENDEI ISLPSTFGSS PRKQGEPSKL 190 200 210 220 230 240 SKGKVNSKIY FVLFTLLIAI PATYYFSLDR FSRVQQGEQS MIEQHNQNVE MNDEHKNENV 250 260 270 280 290 300 IEKLQNKLRT DPNNAETWLQ LGEAYVQNNE FDSALVAYSN AEKLSGSKPN ILGLAATALY 310 320 330 340 350 360 YQAGQQMTQK VEQLLNEALA KDKNEVSSLS LLATIALENR QYQQAGMYLQ QLLDSGNAAV 370 380 DRRSVIQRMK MLDFLQRGEK GQNP

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22592.1.
PIR	H64161.
RefSeq	NP_439094.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44942.
SMR	P44942. Positions 37-114.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0934.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22592; AAC22592; HI_0934.
GeneID	949938.
KEGG	hin:HI0934.
PATRIC	20190525. VBIHaeInf48452_0975.
Phylogenomic databases
eggNOG	COG3088.
KO	K04017. K04018.
OMA	NSPIARD.
ProtClustDB	CLSK808468.
Family and domain databases
Gene3D	1.25.40.10. 2 hits.
InterPro	IPR005616. Cyt_c_biogenesis_CcmH. IPR017565. For-dep_Cytc_NO2Rdtase_NrfF. IPR013026. TPR-contain_dom. IPR011990. TPR-like_helical. IPR019734. TPR_repeat. [Graphical view]
Pfam	PF03918. CcmH. 1 hit. [Graphical view]
TIGRFAMs	TIGR03147. cyt_nit_nrfF. 1 hit.
PROSITE	PS50005. TPR. 1 hit. PS50293. TPR_REGION. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

NRFF_HAEIN

Accession

Primary (citable) accession number: P44942

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	June 6, 2002
Last modified:	May 1, 2013
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program