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Protein

dTDP-glucose 4,6-dehydratase

Gene

rffG

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction.By similarity

Catalytic activityi

dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O.By similarity

Cofactori

NAD+By similarityNote: Binds 1 NAD+ per subunit.By similarity

Pathwayi: dTDP-L-rhamnose biosynthesis

This protein is involved in the pathway dTDP-L-rhamnose biosynthesis, which is part of Carbohydrate biosynthesis.By similarity
View all proteins of this organism that are known to be involved in the pathway dTDP-L-rhamnose biosynthesis and in Carbohydrate biosynthesis.

Pathwayi: LPS O-antigen biosynthesis

This protein is involved in the pathway LPS O-antigen biosynthesis, which is part of Bacterial outer membrane biogenesis.By similarity
View all proteins of this organism that are known to be involved in the pathway LPS O-antigen biosynthesis and in Bacterial outer membrane biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei85Substrate; via carbonyl oxygenBy similarity1
Binding sitei100NADBy similarity1
Binding sitei134SubstrateBy similarity1
Active sitei135Proton donorBy similarity1
Active sitei136Proton acceptorBy similarity1
Active sitei160Proton acceptorBy similarity1
Binding sitei189SubstrateBy similarity1
Binding sitei190NAD; via amide nitrogenBy similarity1
Binding sitei224SubstrateBy similarity1
Binding sitei259SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi12 – 13NADBy similarity2
Nucleotide bindingi33 – 36NADBy similarity4
Nucleotide bindingi59 – 60NADBy similarity2
Nucleotide bindingi81 – 85NADBy similarity5
Nucleotide bindingi160 – 164NADBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processLipopolysaccharide biosynthesis
LigandNAD

Enzyme and pathway databases

UniPathwayiUPA00124
UPA00281

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-glucose 4,6-dehydrataseBy similarity (EC:4.2.1.46By similarity)
Gene namesi
Name:rffG
Ordered Locus Names:HI_0873
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001832371 – 338dTDP-glucose 4,6-dehydrataseAdd BLAST338

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0873

Structurei

3D structure databases

ProteinModelPortaliP44914
SMRiP44914
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni199 – 200Substrate bindingBy similarity2
Regioni215 – 217Substrate bindingBy similarity3
Regioni293 – 297Substrate bindingBy similarity5

Sequence similaritiesi

Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.By similarity

Phylogenomic databases

eggNOGiENOG4105C1B Bacteria
COG1088 LUCA
KOiK01710
OMAiKLIPLMC

Family and domain databases

InterProiView protein in InterPro
IPR005888 dTDP_Gluc_deHydtase
IPR016040 NAD(P)-bd_dom
IPR036291 NAD(P)-bd_dom_sf
PfamiView protein in Pfam
PF16363 GDP_Man_Dehyd, 1 hit
SUPFAMiSSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR01181 dTDP_gluc_dehyt, 1 hit

Sequencei

Sequence statusi: Complete.

P44914-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MMNILVTGGS GFIGSALIRY IINHTQDFVI NIDKLTYAAN QSALREVENN
60 70 80 90 100
PRYVFEKVDI CDLNVIENIF EKYQPDAVMH LAAESHVDRS ISGAADFVQT
110 120 130 140 150
NIVGTYTLLE VAKNYWHTLD EAKKTTFRFH HISTDEVYGD LSLSEPAFTE
160 170 180 190 200
QSPYHPSSPY SASKAASNHL VQAWHRTYGL PVIITNSSNN YGAYQHAEKL
210 220 230 240 250
IPLMISNAVM GKPLPIYGDG QQIRDWLFVE DHVQASYLVL TKGRVGENYN
260 270 280 290 300
IGGNCEKTNL EVVKRICQLL EELAPSKPNH IKYYEDLMTF VKDRPGHDVR
310 320 330
YSLDCSKIHA ELGWQPQITF EQGLRQTVKW YLFNSSSS
Length:338
Mass (Da):38,329
Last modified:November 1, 1995 - v1
Checksum:iB44BCC29D6C7D04B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22531.1
PIRiC64099
RefSeqiNP_439034.2, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22531; AAC22531; HI_0873
GeneIDi949510
KEGGihin:HI0873
PATRICifig|71421.8.peg.914

Similar proteinsi

Entry informationi

Entry nameiRMLB_HAEIN
AccessioniPrimary (citable) accession number: P44914
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 23, 2018
This is version 111 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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