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P44914

- RMLB_HAEIN

UniProt

P44914 - RMLB_HAEIN

Protein

dTDP-glucose 4,6-dehydratase

Gene

rffG

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction.By similarity

    Catalytic activityi

    dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O.

    Cofactori

    Binds 1 NAD per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei81 – 811NAD; via carbonyl oxygenBy similarity
    Binding sitei85 – 851Substrate; via carbonyl oxygenBy similarity
    Binding sitei100 – 1001NADBy similarity
    Active sitei135 – 1351Proton donorBy similarity
    Active sitei136 – 1361Proton acceptorBy similarity
    Active sitei160 – 1601Proton acceptorBy similarity
    Binding sitei189 – 1891SubstrateBy similarity
    Binding sitei190 – 1901NAD; via amide nitrogenBy similarity
    Binding sitei224 – 2241SubstrateBy similarity
    Binding sitei259 – 2591SubstrateBy similarity

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi8 – 147NADSequence Analysis
    Nucleotide bindingi33 – 364NADBy similarity
    Nucleotide bindingi59 – 602NADBy similarity
    Nucleotide bindingi160 – 1645NADBy similarity

    GO - Molecular functioni

    1. coenzyme binding Source: InterPro
    2. dTDP-glucose 4,6-dehydratase activity Source: UniProtKB

    GO - Biological processi

    1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
    2. extracellular polysaccharide biosynthetic process Source: UniProtKB
    3. lipopolysaccharide biosynthetic process Source: UniProtKB
    4. O antigen biosynthetic process Source: UniProtKB-UniPathway

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Lipopolysaccharide biosynthesis

    Keywords - Ligandi

    NAD

    Enzyme and pathway databases

    UniPathwayiUPA00124.
    UPA00281.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    dTDP-glucose 4,6-dehydratase (EC:4.2.1.46)
    Gene namesi
    Name:rffG
    Ordered Locus Names:HI_0873
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 338338dTDP-glucose 4,6-dehydratasePRO_0000183237Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi71421.HI0873.

    Structurei

    3D structure databases

    ProteinModelPortaliP44914.
    SMRiP44914. Positions 1-336.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni134 – 1363Substrate bindingBy similarity
    Regioni199 – 2002Substrate bindingBy similarity
    Regioni215 – 2173Substrate bindingBy similarity
    Regioni294 – 2974Substrate bindingBy similarity

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1088.
    KOiK01710.
    OMAiNANIEFR.
    OrthoDBiEOG6PZXCX.

    Family and domain databases

    Gene3Di3.40.50.720. 1 hit.
    InterProiIPR005888. dTDP_Gluc_deHydtase.
    IPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view]
    PANTHERiPTHR10366:SF41. PTHR10366:SF41. 1 hit.
    PfamiPF01370. Epimerase. 1 hit.
    [Graphical view]
    TIGRFAMsiTIGR01181. dTDP_gluc_dehyt. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P44914-1 [UniParc]FASTAAdd to Basket

    « Hide

    MMNILVTGGS GFIGSALIRY IINHTQDFVI NIDKLTYAAN QSALREVENN    50
    PRYVFEKVDI CDLNVIENIF EKYQPDAVMH LAAESHVDRS ISGAADFVQT 100
    NIVGTYTLLE VAKNYWHTLD EAKKTTFRFH HISTDEVYGD LSLSEPAFTE 150
    QSPYHPSSPY SASKAASNHL VQAWHRTYGL PVIITNSSNN YGAYQHAEKL 200
    IPLMISNAVM GKPLPIYGDG QQIRDWLFVE DHVQASYLVL TKGRVGENYN 250
    IGGNCEKTNL EVVKRICQLL EELAPSKPNH IKYYEDLMTF VKDRPGHDVR 300
    YSLDCSKIHA ELGWQPQITF EQGLRQTVKW YLFNSSSS 338
    Length:338
    Mass (Da):38,329
    Last modified:November 1, 1995 - v1
    Checksum:iB44BCC29D6C7D04B
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22531.1.
    PIRiC64099.
    RefSeqiNP_439034.2. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22531; AAC22531; HI_0873.
    GeneIDi949510.
    KEGGihin:HI0873.
    PATRICi20190401. VBIHaeInf48452_0914.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22531.1 .
    PIRi C64099.
    RefSeqi NP_439034.2. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44914.
    SMRi P44914. Positions 1-336.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0873.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22531 ; AAC22531 ; HI_0873 .
    GeneIDi 949510.
    KEGGi hin:HI0873.
    PATRICi 20190401. VBIHaeInf48452_0914.

    Phylogenomic databases

    eggNOGi COG1088.
    KOi K01710.
    OMAi NANIEFR.
    OrthoDBi EOG6PZXCX.

    Enzyme and pathway databases

    UniPathwayi UPA00124 .
    UPA00281 .

    Family and domain databases

    Gene3Di 3.40.50.720. 1 hit.
    InterProi IPR005888. dTDP_Gluc_deHydtase.
    IPR001509. Epimerase_deHydtase.
    IPR016040. NAD(P)-bd_dom.
    [Graphical view ]
    PANTHERi PTHR10366:SF41. PTHR10366:SF41. 1 hit.
    Pfami PF01370. Epimerase. 1 hit.
    [Graphical view ]
    TIGRFAMsi TIGR01181. dTDP_gluc_dehyt. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiRMLB_HAEIN
    AccessioniPrimary (citable) accession number: P44914
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 96 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3