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P44914 (RMLB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 22, 2014. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
dTDP-glucose 4,6-dehydratase

EC=4.2.1.46
Gene names
Name:rffG
Ordered Locus Names:HI_0873
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length338 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction By similarity.

Catalytic activity

dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O.

Cofactor

Binds 1 NAD per subunit By similarity.

Pathway

Carbohydrate biosynthesis; dTDP-L-rhamnose biosynthesis.

Bacterial outer membrane biogenesis; LPS O-antigen biosynthesis.

Subunit structure

Homodimer By similarity.

Sequence similarities

Belongs to the NAD(P)-dependent epimerase/dehydratase family. dTDP-glucose dehydratase subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 338338dTDP-glucose 4,6-dehydratase
PRO_0000183237

Regions

Nucleotide binding8 – 147NAD Potential
Nucleotide binding33 – 364NAD By similarity
Nucleotide binding59 – 602NAD By similarity
Nucleotide binding160 – 1645NAD By similarity
Region134 – 1363Substrate binding By similarity
Region199 – 2002Substrate binding By similarity
Region215 – 2173Substrate binding By similarity
Region294 – 2974Substrate binding By similarity

Sites

Active site1351Proton donor By similarity
Active site1361Proton acceptor By similarity
Active site1601Proton acceptor By similarity
Binding site811NAD; via carbonyl oxygen By similarity
Binding site851Substrate; via carbonyl oxygen By similarity
Binding site1001NAD By similarity
Binding site1891Substrate By similarity
Binding site1901NAD; via amide nitrogen By similarity
Binding site2241Substrate By similarity
Binding site2591Substrate By similarity

Sequences

Sequence LengthMass (Da)Tools
P44914 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: B44BCC29D6C7D04B

FASTA33838,329
        10         20         30         40         50         60 
MMNILVTGGS GFIGSALIRY IINHTQDFVI NIDKLTYAAN QSALREVENN PRYVFEKVDI 

        70         80         90        100        110        120 
CDLNVIENIF EKYQPDAVMH LAAESHVDRS ISGAADFVQT NIVGTYTLLE VAKNYWHTLD 

       130        140        150        160        170        180 
EAKKTTFRFH HISTDEVYGD LSLSEPAFTE QSPYHPSSPY SASKAASNHL VQAWHRTYGL 

       190        200        210        220        230        240 
PVIITNSSNN YGAYQHAEKL IPLMISNAVM GKPLPIYGDG QQIRDWLFVE DHVQASYLVL 

       250        260        270        280        290        300 
TKGRVGENYN IGGNCEKTNL EVVKRICQLL EELAPSKPNH IKYYEDLMTF VKDRPGHDVR 

       310        320        330 
YSLDCSKIHA ELGWQPQITF EQGLRQTVKW YLFNSSSS 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22531.1.
PIRC64099.
RefSeqNP_439034.2. NC_000907.1.

3D structure databases

ProteinModelPortalP44914.
SMRP44914. Positions 1-336.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0873.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22531; AAC22531; HI_0873.
GeneID949510.
KEGGhin:HI0873.
PATRIC20190401. VBIHaeInf48452_0914.

Phylogenomic databases

eggNOGCOG1088.
KOK01710.
OMATIDWYRE.
OrthoDBEOG6PZXCX.
ProtClustDBCLSK824762.

Enzyme and pathway databases

UniPathwayUPA00124.
UPA00281.

Family and domain databases

Gene3D3.40.50.720. 1 hit.
InterProIPR005888. dTDP_Gluc_deHydtase.
IPR001509. Epimerase_deHydtase.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERPTHR10366:SF41. PTHR10366:SF41. 1 hit.
PfamPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsTIGR01181. dTDP_gluc_dehyt. 1 hit.
ProtoNetSearch...

Entry information

Entry nameRMLB_HAEIN
AccessionPrimary (citable) accession number: P44914
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 22, 2014
This is version 94 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names