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P44914

- RMLB_HAEIN

UniProt

P44914 - RMLB_HAEIN

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Protein

dTDP-glucose 4,6-dehydratase

Gene

rffG

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Catalyzes the dehydration of dTDP-D-glucose to form dTDP-6-deoxy-D-xylo-4-hexulose via a three-step process involving oxidation, dehydration and reduction.By similarity

Catalytic activityi

dTDP-alpha-D-glucose = dTDP-4-dehydro-6-deoxy-alpha-D-glucose + H2O.

Cofactori

NAD(+)By similarityNote: Binds 1 NAD(+) per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei81 – 811NAD; via carbonyl oxygenBy similarity
Binding sitei85 – 851Substrate; via carbonyl oxygenBy similarity
Binding sitei100 – 1001NADBy similarity
Active sitei135 – 1351Proton donorBy similarity
Active sitei136 – 1361Proton acceptorBy similarity
Active sitei160 – 1601Proton acceptorBy similarity
Binding sitei189 – 1891SubstrateBy similarity
Binding sitei190 – 1901NAD; via amide nitrogenBy similarity
Binding sitei224 – 2241SubstrateBy similarity
Binding sitei259 – 2591SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi8 – 147NADSequence Analysis
Nucleotide bindingi33 – 364NADBy similarity
Nucleotide bindingi59 – 602NADBy similarity
Nucleotide bindingi160 – 1645NADBy similarity

GO - Molecular functioni

  1. coenzyme binding Source: InterPro
  2. dTDP-glucose 4,6-dehydratase activity Source: UniProtKB

GO - Biological processi

  1. dTDP-rhamnose biosynthetic process Source: UniProtKB-UniPathway
  2. extracellular polysaccharide biosynthetic process Source: UniProtKB
  3. lipopolysaccharide biosynthetic process Source: UniProtKB
  4. O antigen biosynthetic process Source: UniProtKB-UniPathway
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Lipopolysaccharide biosynthesis

Keywords - Ligandi

NAD

Enzyme and pathway databases

UniPathwayiUPA00124.
UPA00281.

Names & Taxonomyi

Protein namesi
Recommended name:
dTDP-glucose 4,6-dehydratase (EC:4.2.1.46)
Gene namesi
Name:rffG
Ordered Locus Names:HI_0873
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338dTDP-glucose 4,6-dehydratasePRO_0000183237Add
BLAST

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0873.

Structurei

3D structure databases

ProteinModelPortaliP44914.
SMRiP44914. Positions 1-336.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni134 – 1363Substrate bindingBy similarity
Regioni199 – 2002Substrate bindingBy similarity
Regioni215 – 2173Substrate bindingBy similarity
Regioni294 – 2974Substrate bindingBy similarity

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG1088.
KOiK01710.
OMAiNANIEFR.
OrthoDBiEOG6PZXCX.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR005888. dTDP_Gluc_deHydtase.
IPR001509. Epimerase_deHydtase_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view]
PANTHERiPTHR10366:SF41. PTHR10366:SF41. 1 hit.
PfamiPF01370. Epimerase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR01181. dTDP_gluc_dehyt. 1 hit.

Sequencei

Sequence statusi: Complete.

P44914-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MMNILVTGGS GFIGSALIRY IINHTQDFVI NIDKLTYAAN QSALREVENN
60 70 80 90 100
PRYVFEKVDI CDLNVIENIF EKYQPDAVMH LAAESHVDRS ISGAADFVQT
110 120 130 140 150
NIVGTYTLLE VAKNYWHTLD EAKKTTFRFH HISTDEVYGD LSLSEPAFTE
160 170 180 190 200
QSPYHPSSPY SASKAASNHL VQAWHRTYGL PVIITNSSNN YGAYQHAEKL
210 220 230 240 250
IPLMISNAVM GKPLPIYGDG QQIRDWLFVE DHVQASYLVL TKGRVGENYN
260 270 280 290 300
IGGNCEKTNL EVVKRICQLL EELAPSKPNH IKYYEDLMTF VKDRPGHDVR
310 320 330
YSLDCSKIHA ELGWQPQITF EQGLRQTVKW YLFNSSSS
Length:338
Mass (Da):38,329
Last modified:November 1, 1995 - v1
Checksum:iB44BCC29D6C7D04B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22531.1.
PIRiC64099.
RefSeqiNP_439034.2. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22531; AAC22531; HI_0873.
GeneIDi949510.
KEGGihin:HI0873.
PATRICi20190401. VBIHaeInf48452_0914.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22531.1 .
PIRi C64099.
RefSeqi NP_439034.2. NC_000907.1.

3D structure databases

ProteinModelPortali P44914.
SMRi P44914. Positions 1-336.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0873.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22531 ; AAC22531 ; HI_0873 .
GeneIDi 949510.
KEGGi hin:HI0873.
PATRICi 20190401. VBIHaeInf48452_0914.

Phylogenomic databases

eggNOGi COG1088.
KOi K01710.
OMAi NANIEFR.
OrthoDBi EOG6PZXCX.

Enzyme and pathway databases

UniPathwayi UPA00124 .
UPA00281 .

Family and domain databases

Gene3Di 3.40.50.720. 1 hit.
InterProi IPR005888. dTDP_Gluc_deHydtase.
IPR001509. Epimerase_deHydtase_N.
IPR016040. NAD(P)-bd_dom.
[Graphical view ]
PANTHERi PTHR10366:SF41. PTHR10366:SF41. 1 hit.
Pfami PF01370. Epimerase. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR01181. dTDP_gluc_dehyt. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiRMLB_HAEIN
AccessioniPrimary (citable) accession number: P44914
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 26, 2014
This is version 98 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3