Reviewed, UniProtKB/Swiss-Prot P44893 (FRDB_HAEIN)

Names and origin

Protein names

Recommended name:
    Fumarate reductase iron-sulfur subunit
    EC=1.3.99.1

Gene names

Name:	frdB
Ordered Locus Names:	HI0834

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Succinate + acceptor = fumarate + reduced acceptor.
Cofactor	Binds 1 2Fe-2S cluster By similarity. Binds 1 3Fe-4S cluster By similarity. Binds 1 4Fe-4S cluster By similarity.
Subunit structure	Fumarate dehydrogenase forms part of an enzyme complex containing four subunits: a flavoprotein, an iron-sulfur, and two hydrophobic anchor proteins By similarity.
Sequence similarities	Belongs to the succinate dehydrogenase/fumarate reductase iron-sulfur protein family. Contains 1 2Fe-2S ferredoxin-type domain. Contains 1 4Fe-4S ferredoxin-type domain.

Ontologies

Keywords
Biological process	Electron transport Transport Tricarboxylic acid cycle
Ligand	2Fe-2S 3Fe-4S 4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW transport Inferred from electronic annotation. Source: UniProtKB-KW tricarboxylic acid cycle Inferred from electronic annotation. Source: InterPro
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW electron carrier activity Inferred from electronic annotation. Source: InterPro iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW succinate dehydrogenase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Fumarate reductase iron-sulfur subunit		PRO_0000158702
Regions
	Domain	7 – 97	91	2Fe-2S ferredoxin-type
	Domain	151 – 180	30	4Fe-4S ferredoxin-type
Sites
	Metal binding	58	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	63	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	66	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	78	1	Iron-sulfur 1 (2Fe-2S) By similarity
	Metal binding	160	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	163	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	166	1	Iron-sulfur 2 (4Fe-4S) By similarity
	Metal binding	170	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	216	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	222	1	Iron-sulfur 3 (3Fe-4S) By similarity
	Metal binding	226	1	Iron-sulfur 2 (4Fe-4S) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44893-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 42E3851D0B6B355E	FASTA	256	28,668
	10 20 30 40 50 60 MANSPVMNVE VLRYNPEIDQ EPHLSTYQVP YDNQTSLLDA LGYIKDKLEP SLSYRWSCRM 70 80 90 100 110 120 AICGSCGMMV NNKPKLACKT FLRDYSGHMR IEPLANFPIE RDLVVDLSHF IESLEAIKPY 130 140 150 160 170 180 IIGNEAPALD GKPHPSKELQ VSRTKQTPAQ LEKYRQFSMC INCGLCYAAC PQFGLNPEFL 190 200 210 220 230 240 GPAAITMAHR YNLDNRDHGK AKRMSLLNGK NGVWSCTFVG YCSEVCPKHV DPASAINQGK 250 VESAKDYVIS MLKPKG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC22492.1.
RefSeq	NP_438994.1.
3D structure databases
HSSP	HSSP built from PDB template 1KF6 based on UniProtKB P00364.
SMR	P44893. Positions 7-255.
ModBase	Search...
Genome annotation databases
GeneID	949848.
GenomeReviews	Gene locus HI0834 in contig L42023_GR.
KEGG	hin:HI0834.
TIGR	HI0834.
Phylogenomic databases
HOGENOM	P44893.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0834-MON.
Family and domain databases
InterPro	IPR006058. 2Fe2S_fd_BS. IPR001450. 4Fe4S_Fe_S_bd. IPR012675. b-grasp_ferredoxin-like. IPR001041. Ferredoxin. IPR012285. Fum_reductase_C. IPR004489. Succ_DHase/fum_Rdtase_Fe-S. [Graphical view]
Gene3D	G3DSA:3.10.20.30. Ferredoxin_fold. 1 hit. G3DSA:1.10.1060.10. Fum_reductase_C. 1 hit.
Pfam	PF00111. Fer2. 1 hit. PF00037. Fer4. 1 hit. [Graphical view]
PRINTS	PR00353. 4FE4SFRDOXIN.
TIGRFAMs	TIGR00384. dhsB. 1 hit.
PROSITE	PS00197. 2FE2S_FER_1. 1 hit. PS51085. 2FE2S_FER_2. 1 hit. PS00198. 4FE4S_FER_1. 1 hit. PS51379. 4FE4S_FER_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRDB_HAEIN

Accession

Primary (citable) accession number: P44893

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 25, 2008
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)