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Protein

tRNA (cytidine(34)-2'-O)-methyltransferase

Gene

trmL

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA.UniRule annotation
S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei78S-adenosyl-L-methionine; via carbonyl oxygenUniRule annotation1 Publication1
Binding sitei100S-adenosyl-L-methionine; via amide nitrogenUniRule annotation1 Publication1
Binding sitei122S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenUniRule annotation1 Publication1
Binding sitei130S-adenosyl-L-methionineUniRule annotation1 Publication1

GO - Molecular functioni

  • identical protein binding Source: IntAct
  • RNA binding Source: InterPro
  • RNA methyltransferase activity Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processtRNA processing
LigandS-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-806-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (cytidine(34)-2'-O)-methyltransferaseUniRule annotation (EC:2.1.1.207UniRule annotation)
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmLUniRule annotation
Gene namesi
Name:trmLUniRule annotation
Ordered Locus Names:HI_0766
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB01752 S-Adenosyl-L-Homocysteine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001598191 – 160tRNA (cytidine(34)-2'-O)-methyltransferaseAdd BLAST160

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself4EBI-15618808,EBI-15618808

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

DIPiDIP-29173N
STRINGi71421.HI0766

Structurei

Secondary structure

1160
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi3 – 8Combined sources6
Helixi12 – 25Combined sources14
Beta strandi28 – 34Combined sources7
Helixi42 – 45Combined sources4
Turni46 – 48Combined sources3
Helixi51 – 53Combined sources3
Beta strandi54 – 56Combined sources3
Beta strandi58 – 62Combined sources5
Helixi63 – 70Combined sources8
Beta strandi73 – 78Combined sources6
Beta strandi83 – 85Combined sources3
Helixi86 – 88Combined sources3
Beta strandi95 – 99Combined sources5
Turni102 – 104Combined sources3
Helixi108 – 111Combined sources4
Helixi116 – 118Combined sources3
Beta strandi119 – 121Combined sources3
Helixi133 – 147Combined sources15
Turni148 – 152Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J85X-ray2.00A1-160[»]
1MXIX-ray1.70A1-160[»]
ProteinModelPortaliP44868
SMRiP44868
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44868

Family & Domainsi

Sequence similaritiesi

Belongs to the class IV-like SAM-binding methyltransferase superfamily. RNA methyltransferase TrmH family. TrmL subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108UIQ Bacteria
COG0219 LUCA
KOiK03216
OMAiAGLDYWH
PhylomeDBiP44868

Family and domain databases

Gene3Di3.40.1280.10, 1 hit
HAMAPiMF_01885 tRNA_methyltr_TrmL, 1 hit
InterProiView protein in InterPro
IPR029028 Alpha/beta_knot_MTases
IPR001537 SpoU_MeTrfase
IPR016914 tRNA_cyt/urid_MeTfrase
IPR029026 tRNA_m1G_MTases_N
PANTHERiPTHR42971 PTHR42971, 1 hit
PfamiView protein in Pfam
PF00588 SpoU_methylase, 1 hit
PIRSFiPIRSF029256 SpoU_TrmH_prd, 1 hit
SUPFAMiSSF75217 SSF75217, 1 hit
TIGRFAMsiTIGR00185 tRNA_yibK_trmL, 1 hit

Sequencei

Sequence statusi: Complete.

P44868-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLDIVLYEPE IPQNTGNIIR LCANTGFRLH LIEPLGFTWD DKRLRRSGLD
60 70 80 90 100
YHEFAEIKRH KTFEAFLESE KPKRLFALTT KGCPAHSQVK FKLGDYLMFG
110 120 130 140 150
PETRGIPMSI LNEMPMEQKI RIPMTANSRS MNLSNSVAVT VYEAWRQLGY
160
KGAVNLPEVK
Length:160
Mass (Da):18,401
Last modified:November 1, 1995 - v1
Checksum:i0E09ADB31E575026
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22424.1
PIRiE64158
RefSeqiNP_438925.1, NC_000907.1
WP_005652354.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22424; AAC22424; HI_0766
GeneIDi949782
KEGGihin:HI0766
PATRICifig|71421.8.peg.805

Similar proteinsi

Entry informationi

Entry nameiTRML_HAEIN
AccessioniPrimary (citable) accession number: P44868
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 28, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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