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P44868 (TRML_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 80. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA (cytidine(34)-2'-O)-methyltransferase
EC=2.1.1.207
Alternative name(s):
tRNA (cytidine/uridine-2'-O-)-methyltransferase TrmL
Gene names
Name:trmL
Ordered Locus Names:HI_0766
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length160 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Methylates the ribose at the nucleotide 34 wobble position in the two leucyl isoacceptors tRNA(Leu)(CmAA) and tRNA(Leu)(cmnm5UmAA). Catalyzes the methyl transfer from S-adenosyl-L-methionine to the 2'-OH of the wobble nucleotide By similarity. HAMAP MF_01885

Catalytic activity

S-adenosyl-L-methionine + cytidine(34) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(34) in tRNA. HAMAP MF_01885

S-adenosyl-L-methionine + 5-carboxymethylaminomethyluridine(34) in tRNA(Leu) = S-adenosyl-L-homocysteine + 5-carboxymethylaminomethyl-2'-O-methyluridine(34) in tRNA(Leu). HAMAP MF_01885

Subunit structure

Homodimer. Ref.2

Subcellular location

Cytoplasm Potential HAMAP MF_01885.

Sequence similarities

Belongs to the RNA methyltransferase TrmH family. TrmL subfamily.

Ontologies

Keywords
   Biological processtRNA processing
   Cellular componentCytoplasm
   LigandS-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular functionRNA binding

Inferred from electronic annotation. Source: InterPro

RNA methyltransferase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 160160tRNA (cytidine(34)-2'-O)-methyltransferase HAMAP MF_01885
PRO_0000159819

Sites

Binding site781S-adenosyl-L-methionine; via carbonyl oxygen
Binding site1001S-adenosyl-L-methionine; via amide nitrogen
Binding site1221S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygen
Binding site1301S-adenosyl-L-methionine

Secondary structure

1 .............................. 160
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P44868 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 0E09ADB31E575026

FASTA16018,401
        10         20         30         40         50         60 
MLDIVLYEPE IPQNTGNIIR LCANTGFRLH LIEPLGFTWD DKRLRRSGLD YHEFAEIKRH 

        70         80         90        100        110        120 
KTFEAFLESE KPKRLFALTT KGCPAHSQVK FKLGDYLMFG PETRGIPMSI LNEMPMEQKI 

       130        140        150        160 
RIPMTANSRS MNLSNSVAVT VYEAWRQLGY KGAVNLPEVK

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L42023 Genomic DNA. Translation: AAC22424.1.
PIRE64158.
RefSeqNP_438925.1. NC_000907.1.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1J85X-ray2.00A1-160[»]
1MXIX-ray1.70A1-160[»]
ProteinModelPortalP44868.
SMRP44868. Positions 1-156.
ModBaseSearch...

Protein-protein interaction databases

DIPDIP-29173N.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID949782.
GenomeReviewsGene locus HI_0766 in contig L42023_GR.
KEGGhin:HI0766.
NMPDRfig|71421.1.peg.734.
PATRIC20190183. VBIHaeInf48452_0805.
TIGRHI_0766.

Phylogenomic databases

HOGENOMHBG309728.
OMAEAWRQNG.
ProtClustDBCLSK870515.

Enzyme and pathway databases

BioCycHINF71421:HI_0766-MONOMER.

Family and domain databases

HAMAPMF_01885. tRNA_methyltr_TrmL.
[Tree]
InterProIPR001537. SpoU_MeTrfase.
IPR016914. tRNA_cyt/urid_MeTfrase.
[Graphical view]
KOK03216.
PANTHERPTHR12029:SF26. PTHR12029:SF26. 1 hit.
PfamPF00588. SpoU_methylase. 1 hit.
[Graphical view]
PIRSFPIRSF029256. SpoU_TrmH_prd. 1 hit.
TIGRFAMsTIGR00185. RRNA_methyl_2. 1 hit.
ProtoNetSearch...

Entry information

Entry nameTRML_HAEIN
AccessionPrimary (citable) accession number: P44868
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 80 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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