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Protein

Ketol-acid reductoisomerase (NADP(+))

Gene

ilvC

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the biosynthesis of branched-chain amino acids (BCAA). Catalyzes an alkyl-migration followed by a ketol-acid reduction of (S)-2-acetolactate (S2AL) to yield (R)-2,3-dihydroxy-isovalerate. In the isomerase reaction, S2AL is rearranged via a Mg-dependent methyl migration to produce 3-hydroxy-3-methyl-2-ketobutyrate (HMKB). In the reductase reaction, this 2-ketoacid undergoes a metal-dependent reduction by NADPH to yield (R)-2,3-dihydroxy-isovalerate.UniRule annotation

Catalytic activityi

(2R)-2,3-dihydroxy-3-methylbutanoate + NADP+ = (2S)-2-hydroxy-2-methyl-3-oxobutanoate + NADPH.UniRule annotation
(2R,3R)-2,3-dihydroxy-3-methylpentanoate + NADP+ = (S)-2-hydroxy-2-ethyl-3-oxobutanoate + NADPH.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 2 magnesium ions per subunit.UniRule annotation

Pathwayi: L-isoleucine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-isoleucine from 2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit (ilvH), Acetolactate synthase large subunit (ilvI)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-isoleucine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-isoleucine from 2-oxobutanoate, the pathway L-isoleucine biosynthesis and in Amino-acid biosynthesis.

Pathwayi: L-valine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-valine from pyruvate.UniRule annotation
Proteins known to be involved in the 4 steps of the subpathway in this organism are:
  1. Acetolactate synthase small subunit (ilvH), Acetolactate synthase large subunit (ilvI)
  2. Ketol-acid reductoisomerase (NADP(+)) (ilvC)
  3. Dihydroxy-acid dehydratase (ilvD)
  4. Branched-chain-amino-acid aminotransferase (ilvE)
This subpathway is part of the pathway L-valine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-valine from pyruvate, the pathway L-valine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei68NADPUniRule annotation1
Binding sitei76NADPUniRule annotation1
Binding sitei78NADPUniRule annotation1
Active sitei132UniRule annotation1
Binding sitei158NADP; via amide nitrogenUniRule annotation1
Metal bindingi217Magnesium 1UniRule annotation1
Metal bindingi217Magnesium 2UniRule annotation1
Metal bindingi221Magnesium 1UniRule annotation1
Metal bindingi389Magnesium 2UniRule annotation1
Metal bindingi393Magnesium 2UniRule annotation1
Binding sitei414SubstrateUniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi45 – 48NADPUniRule annotation4
Nucleotide bindingi108 – 110NADPUniRule annotation3

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processAmino-acid biosynthesis, Branched-chain amino acid biosynthesis
LigandMagnesium, Metal-binding, NADP

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-717-MONOMER
UniPathwayiUPA00047; UER00056
UPA00049; UER00060

Names & Taxonomyi

Protein namesi
Recommended name:
Ketol-acid reductoisomerase (NADP(+))UniRule annotation (EC:1.1.1.86UniRule annotation)
Short name:
KARIUniRule annotation
Alternative name(s):
Acetohydroxy-acid isomeroreductaseUniRule annotation
Short name:
AHIRUniRule annotation
Alpha-keto-beta-hydroxylacyl reductoisomeraseUniRule annotation
Ketol-acid reductoisomerase type 2UniRule annotation
Ketol-acid reductoisomerase type IIUniRule annotation
Gene namesi
Name:ilvCUniRule annotation
Ordered Locus Names:HI_0682
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001513142 – 492Ketol-acid reductoisomerase (NADP(+))Add BLAST491

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0682

Structurei

3D structure databases

ProteinModelPortaliP44822
SMRiP44822
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 208KARI N-terminal RossmannPROSITE-ProRule annotationAdd BLAST195
Domaini209 – 344KARI C-terminal knotted 1PROSITE-ProRule annotationAdd BLAST136
Domaini345 – 485KARI C-terminal knotted 2PROSITE-ProRule annotationAdd BLAST141

Sequence similaritiesi

Belongs to the ketol-acid reductoisomerase family.UniRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105C6M Bacteria
COG0059 LUCA
KOiK00053
OMAiKLFEMNR
PhylomeDBiP44822

Family and domain databases

Gene3Di1.10.1040.10, 1 hit
HAMAPiMF_00435 IlvC, 1 hit
InterProiView protein in InterPro
IPR008927 6-PGluconate_DH-like_C_sf
IPR013328 6PGD_dom2
IPR013023 KARI
IPR000506 KARI_C
IPR013116 KARI_N
IPR036291 NAD(P)-bd_dom_sf
PANTHERiPTHR21371 PTHR21371, 2 hits
PfamiView protein in Pfam
PF01450 IlvC, 2 hits
PF07991 IlvN, 1 hit
SUPFAMiSSF48179 SSF48179, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR00465 ilvC, 1 hit
PROSITEiView protein in PROSITE
PS51851 KARI_C, 2 hits
PS51850 KARI_N, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44822-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MANYFNTLNL RQKLDQLGRC RFMDREEFAD EANFLKGKKI VIVGCGAQGL
60 70 80 90 100
NQGLNMRDSG LDISYALRPE AIAEKRASFQ RATENGFKVG TYEELIPTAD
110 120 130 140 150
LVVNLTPDKQ HSKVVADVMP LMKKDSAFGY SHGFNIVEVG EEIRKDITVV
160 170 180 190 200
MVAPKCPGTE VREEYKRGFG VPTLIAVHPE NDPKGEGMAI AKAWAAATGG
210 220 230 240 250
HKAGVLESSF VAEVKSDLMG EQTILCGMLQ AGSIVCYDKL VADGKDPAYA
260 270 280 290 300
GKLIQYGWET ITEALKQGGI TLMMDRLSNS AKLRAFELAE QIKESLGFLY
310 320 330 340 350
YKHMDDIISG HFSATMMADW ANGDKDLFAW REATGKTAFE NAPKYDGKIS
360 370 380 390 400
EQEYFDNGVL MIAMVKAGVE LAFDAMVASG IYEESAYYES LHELPLIANT
410 420 430 440 450
IARKRLYEMN VVISDTAEYG NYLFSNVATP ILAKEIIPNL QKGDLGEPTP
460 470 480 490
AVEVDNITLR DVNDAIRNHP VELIGQELRG YMTDMKRIAV AG
Length:492
Mass (Da):54,239
Last modified:January 23, 2007 - v2
Checksum:i16D504256D7FF18E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22342.1
PIRiB64086
RefSeqiNP_438842.1, NC_000907.1
WP_005654301.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22342; AAC22342; HI_0682
GeneIDi949478
KEGGihin:HI0682
PATRICifig|71421.8.peg.713

Entry informationi

Entry nameiILVC_HAEIN
AccessioniPrimary (citable) accession number: P44822
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 127 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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