Reviewed, UniProtKB/Swiss-Prot P44815 (ISPF_HAEIN)

Names and origin

Protein names

Recommended name:
    2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase
      Short name=MECPS
      Short name=MECDP-synthase
    EC=4.6.1.12

Gene names

Name:	ispF
Ordered Locus Names:	HI0671

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	158 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2-phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP By similarity.
Catalytic activity	2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = 2-C-methyl-D-erythritol 2,4-cyclodiphosphate + CMP. HAMAP MF_00107
Cofactor	Binds 1 divalent metal cation per subunit. HAMAP MF_00107
Pathway	Isoprenoid biosynthesis; isopentenyl-PP biosynthesis via DXP pathway; isopentenyl-PP from 1-deoxy-D-xylulose 5-phosphate: step 4/6. HAMAP MF_00107
Subunit structure	Homotrimer. HAMAP MF_00107
Sequence similarities	Belongs to the ispF family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	Metal-binding
Molecular function	Lyase
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase activity Inferred from electronic annotation. Source: HAMAP metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 158

158

2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase HAMAP MF_00107

PRO_0000189472

Sites

Metal binding

9

1

Divalent metal cation HAMAP MF_00107

Metal binding

11

1

Divalent metal cation HAMAP MF_00107

Metal binding

43

1

Divalent metal cation HAMAP MF_00107

Site

35

1

Transition state stabilizer By similarity

Site

134

1

Transition state stabilizer By similarity

Secondary structure

1

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158

Helix Strand Turn

Details...

Beta strand

2 – 12

11

Beta strand

14 – 21

8

Beta strand

24 – 32

9

Helix

40 – 52

13

Helix

58 – 60

3

Helix

74 – 87

14

Beta strand

90 – 100

11

Helix

107 – 120

14

Helix

125 – 127

3

Beta strand

128 – 133

6

Helix

139 – 142

4

Beta strand

145 – 157

13

Sequences

	Sequence		Length	Mass (Da)
	P44815-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: DC34BF347DEC2EFF	FASTA	158	17,194
	10 20 30 40 50 60 MIRIGHGFDV HAFGEDRPLI IGGVEVPYHT GFIAHSDGDV ALHALTDAIL GAAALGDIGK 70 80 90 100 110 120 LFPDTDMQYK NADSRGLLRE AFRQVQEKGY KIGNVDITII AQAPKMRPHI DAMRAKIAED 130 140 150 LQCDIEQVNV KATTTEKLGF TGRQEGIACE AVALLIRQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Reference map of the low molecular mass proteins of Haemophilus influenzae." Fountoulakis M., Juranville J.-F., Roeder D., Evers S., Berndt P., Langen H. Electrophoresis 19:1819-1827(1998) [PubMed: 9719565] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
[3]	"Structure of 2C-methyl-D-erythrol-2,4-cyclodiphosphate synthase from Haemophilus influenzae: activation by conformational transition." Lehmann C., Lim K., Toedt J., Krajewski W., Howard A., Eisenstein E., Herzberg O. Proteins 49:135-138(2002) [PubMed: 12211023] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.9 ANGSTROMS).

Cross-references

Sequence databases

EMBLGenBankDDBJ

L42023 Genomic DNA. Translation: AAC22331.1.

PIR

F64156.

RefSeq

NP_438831.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JN1	X-ray	2.90	A/B/C	1-158	[»]
1VH8	X-ray	2.35	A/B/C/D/E/F	2-158	[»]
1VHA	X-ray	2.35	A/B/C/D/E/F	2-158	[»]

ModBase

Search...

Genome annotation databases

GeneID

950633.

GenomeReviews

Gene locus HI0671 in contig L42023_GR.

KEGG

hin:HI0671.

NMPDR

fig|71421.1.peg.641.

TIGR

HI0671.

Phylogenomic databases

HOGENOM

P44815.

OMA

P44815. HAICDAL.

Enzyme and pathway databases

BioCyc

HINF71421:HI_0671-MON.

BRENDA

4.6.1.12. 109.

Family and domain databases

HAMAP

MF_00107.
[Tree]

InterPro

IPR003526. MECDP_synthase_core.
[Graphical view]

Gene3D

G3DSA:3.30.1330.50. MECDP_synthase_core. 1 hit.

Pfam

PF02542. YgbB. 1 hit.
[Graphical view]

TIGRFAMs

TIGR00151. ispF. 1 hit.

PROSITE

PS01350. ISPF. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

ISPF_HAEIN

Accession

Primary (citable) accession number: P44815

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 71 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)