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Protein

D-aminoacyl-tRNA deacylase

Gene

dtd

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

An aminoacyl-tRNA editing enzyme that deacylates mischarged D-aminoacyl-tRNAs. Also deacylates mischarged glycyl-tRNA(Ala), protecting cells against glycine mischarging by AlaRS. Acts via tRNA-based rather than protein-based catalysis; rejects L-amino acids rather than detecting D-amino acids in the active site. By recycling D-aminoacyl-tRNA to D-amino acids and free tRNA molecules, this enzyme counteracts the toxicity associated with the formation of D-aminoacyl-tRNA entities in vivo and helps enforce protein L-homochirality.UniRule annotation

Caution

Initially the conserved reside Thr-80 was thought to be a nucleophile (PubMed:12571243); mutagenesis in E.coli and P.falciparum indicates it is not.1 Publication

Catalytic activityi

Glycyl-tRNA(Ala) + H2O = glycine + tRNA(Ala).UniRule annotation
A D-aminoacyl-tRNA + H2O = a D-amino acid + tRNA.UniRule annotation

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding, tRNA-binding

Enzyme and pathway databases

BioCyciHINF71421:G1GJ1-705-MONOMER
BRENDAi3.1.1.96 2529

Names & Taxonomyi

Protein namesi
Recommended name:
D-aminoacyl-tRNA deacylaseUniRule annotation (EC:3.1.1.96UniRule annotation)
Short name:
DTDUniRule annotation
Alternative name(s):
D-tyrosyl-tRNA(Tyr) deacylase1 Publication
Gly-tRNA(Ala) deacylaseUniRule annotation (EC:3.1.1.-UniRule annotation)
Gene namesi
Name:dtdUniRule annotation
Ordered Locus Names:HI_0670
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotationCurated

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001645461 – 144D-aminoacyl-tRNA deacylaseAdd BLAST144

Interactioni

Subunit structurei

Homodimer.UniRule annotation1 Publication

Protein-protein interaction databases

STRINGi71421.HI0670

Structurei

Secondary structure

1144
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi2 – 15Combined sources14
Beta strandi18 – 32Combined sources15
Helixi39 – 51Combined sources13
Beta strandi62 – 64Combined sources3
Turni66 – 70Combined sources5
Beta strandi72 – 77Combined sources6
Helixi79 – 82Combined sources4
Helixi99 – 114Combined sources16
Beta strandi119 – 121Combined sources3
Beta strandi129 – 143Combined sources15

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1J7GX-ray1.64A1-144[»]
ProteinModelPortaliP44814
SMRiP44814
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44814

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi136 – 137Gly-cisPro motif, important for rejection of L-amino acidsUniRule annotation2

Domaini

A Gly-cisPro motif from one monomer fits into the active site of the other monomer to allow specific chiral rejection of L-amino acids.UniRule annotation

Sequence similaritiesi

Belongs to the DTD family.UniRule annotationCurated

Phylogenomic databases

eggNOGiENOG4108YYA Bacteria
COG1490 LUCA
KOiK07560
OMAiGVFQAHM
PhylomeDBiP44814

Family and domain databases

CDDicd00563 Dtyr_deacylase, 1 hit
Gene3Di3.50.80.10, 1 hit
HAMAPiMF_00518 Deacylase_Dtd, 1 hit
InterProiView protein in InterPro
IPR003732 Daa-tRNA_deacyls_DTD
IPR023509 DTD-like_sf
PANTHERiPTHR10472 PTHR10472, 1 hit
PfamiView protein in Pfam
PF02580 Tyr_Deacylase, 1 hit
SUPFAMiSSF69500 SSF69500, 1 hit
TIGRFAMsiTIGR00256 TIGR00256, 1 hit

Sequencei

Sequence statusi: Complete.

P44814-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MIALIQRVSQ AKVDVKGETI GKIGKGLLVL LGVEKEDNRE KADKLAEKVL
60 70 80 90 100
NYRIFSDEND KMNLNVQQAQ GELLIVSQFT LAADTQKGLR PSFSKGASPA
110 120 130 140
LANELYEYFI QKCAEKLPVS TGQFAADMQV SLTNDGPVTF WLNV
Length:144
Mass (Da):15,862
Last modified:November 1, 1995 - v1
Checksum:iD8F84702EF5A3CBB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22330.1
PIRiE64156
RefSeqiNP_438830.1, NC_000907.1
WP_005694618.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22330; AAC22330; HI_0670
GeneIDi949713
KEGGihin:HI0670
PATRICifig|71421.8.peg.700

Similar proteinsi

Entry informationi

Entry nameiDTD_HAEIN
AccessioniPrimary (citable) accession number: P44814
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 28, 2018
This is version 120 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health