Reviewed, UniProtKB/Swiss-Prot P44798 (TORZ_HAEIN)

Names and origin

Protein names

Recommended name:
    Trimethylamine-N-oxide reductase
      Short name=TMAO reductase
      Short name=Trimethylamine oxidase
    EC=1.7.2.3

Gene names

Name:	torZ
Synonyms:	bisC
Ordered Locus Names:	HI0643

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	825 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Reduces trimethylamine-N-oxide (TMAO) into trimethylamine; an anaerobic reaction coupled to energy-yielding reactions By similarity.
Catalytic activity	Trimethylamine + 2 (ferricytochrome c)-subunit + H2O = trimethylamine N-oxide + 2 (ferrocytochrome c)-subunit + 2 H+.
Cofactor	Molybdenum (molybdopterin) By similarity.
Subcellular location	Periplasm By similarity.
Post-translational modification	Predicted to be exported by the Tat system. The position of the signal peptide cleavage has not been experimentally proven.
Sequence similarities	Belongs to the prokaryotic molybdopterin-containing oxidoreductase family.
Caution	Was originally (Ref.1) assigned to be a biotin sulfoxide reductase hence the original gene designation of bisC.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Signal
Ligand	Molybdenum
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	electron carrier activity Inferred from electronic annotation. Source: InterPro molybdenum ion binding Inferred from electronic annotation. Source: UniProtKB-KW trimethylamine-N-oxide reductase (cytochrome c) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 40	40	Tat-type signal Potential
	Chain	41 – 825	785	Trimethylamine-N-oxide reductase		PRO_0000019166

Sequences

	Sequence		Length	Mass (Da)
	P44798-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4254287D42B45E5B	FASTA	825	91,053
	10 20 30 40 50 60 MKKNNVNEQR RDFLKKTSLG VAGSALSGGM VGVVSKSAVA KEAEMKTVVT AAHWGSIGVV 70 80 90 100 110 120 VQDGKVVKSG PAIEPAVPNE LQTVVADQLY SERRVKCPMV RKGFLANPGK SDTTMRGRDE 130 140 150 160 170 180 WVRVSWDEAL DLVHNQLKRV RDEHGSTGIF AGSYGWFSCG SLHASRTLLQ RYMNATGGFV 190 200 210 220 230 240 GHKGDYSTGA AQVIMPHVLG TIEVYEQQTS WESILESSDI IVLWSANPLT TMRIAWMSTD 250 260 270 280 290 300 QKGIEYFKKF QASGKRIICI DPQKSETCQM LNAEWIPVNT ATDVPLMLGI AHTLVEQGKH 310 320 330 340 350 360 DKDFLKKYTS GYAKFEEYLL GKTDGQPKTA EWAAKICGVP AETIKQLAAD FASKRTMLMG 370 380 390 400 410 420 GWGMQRQRHG EQTHWMLVTL ASMLGQIGLP GGGFGLSYHY SNGGVPTATG GIIGSITASP 430 440 450 460 470 480 SGKAGAKTWL DDTSKSAFPL ARIADVLLHP GKKIQYNGTE ITYPDIKAVY WAGGNPFVHH 490 500 510 520 530 540 QDTNTLVKAF QKPDVVIVNE VNWTPTARMA DIVLPATTSY ERNDLTMAGD YSMMSVYPMK 550 560 570 580 590 600 QVVPPQFEAK NDYDIFVELA KRAGVEEQYT EGKTEMEWLE EFYNAAFSAA RANRVAMPRF 610 620 630 640 650 660 DKFWAENKPL SFEAGEAAKK WVRYGEFRED PLLNPLGTPS GKIEIFSDVV EKMNYNDCKG 670 680 690 700 710 720 HPSWMEPEEF AGNVTEEYPL ALVTPHPYYR LHSQLAHTSL RQKYAVNDRE PVMIHPEDAA 730 740 750 760 770 780 ARGIKDGDIV RIHSKRGQVL AGAAVTENII KGTVALHEGA WYDPMYLGES EKPLCKNGCA 790 800 810 820 NVLTRDEGTS KLAQGNSPNT CIVQIEKFIG VAPEVTVFKQ PKQVA

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22303.1.
PIR	H64083.
RefSeq	NP_438803.1.
3D structure databases
SMR	P44798. Positions 46-823.
ModBase	Search...
Genome annotation databases
GeneID	950790.
GenomeReviews	Gene locus HI0643 in contig L42023_GR.
KEGG	hin:HI0643.
NMPDR	fig|71421.1.peg.613.
TIGR	HI0643.
Phylogenomic databases
HOGENOM	HBG304064.
OMA	MIREEVM.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0643-MONOMER.
BRENDA	1.7.2.3. 109.
Family and domain databases
InterPro	IPR009010. Asp_de-COase-like_fold. IPR006658. BisC. IPR006657. MoPterin_dinucl-bd_dom. IPR006656. Mopterin_OxRdtase. IPR006655. Mopterin_OxRdtase_prok_CS. IPR006311. TAT_signal. IPR017909. Twin_arg_translocation_Tat. [Graphical view]
Gene3D	G3DSA:2.40.40.20. Asp_decarboxylase-like_fold. 1 hit.
Pfam	PF00384. Molybdopterin. 1 hit. PF01568. Molydop_binding. 1 hit. [Graphical view]
TIGRFAMs	TIGR00509. bisC_fam. 1 hit. TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS00551. MOLYBDOPTERIN_PROK_1. False negative. PS00490. MOLYBDOPTERIN_PROK_2. 1 hit. PS00932. MOLYBDOPTERIN_PROK_3. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

TORZ_HAEIN

Accession

Primary (citable) accession number: P44798

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 75 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)