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P44791

- RSEA_HAEIN

UniProt

P44791 - RSEA_HAEIN

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Protein

Anti-sigma-E factor RseA

Gene

rseA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

An anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-E (RpoE). ECF sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein is first cut periplasmically (site-1 protease, S1P, DegS), then within the membrane itself (site-2 protease, S2P, RseP), while cytoplasmic proteases finish degrading the anti-sigma factor, liberating sigma-E By similarity.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei148 – 1492Cleavage; by DegSBy similarity

GO - Molecular functioni

  1. sigma factor antagonist activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. response to stress Source: InterPro
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Anti-sigma-E factor RseA
Alternative name(s):
Regulator of SigE
Sigma-E anti-sigma factor RseA
Sigma-E factor negative regulatory protein
Gene namesi
Name:rseA
Synonyms:mclA
Ordered Locus Names:HI_0629
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cell inner membrane By similarity; Single-pass type II membrane protein By similarity
Note: Following cleavage by DegS the large fragment of the protein is still in the inner membrane and retains its anti-sigma-E activity.By similarity

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Anti-sigma-E factor RseAPRO_0000097482Add
BLAST

Post-translational modificationi

Sequentially cleaved by DegS (a site-1 protease) in its periplasmic domain between Val-147 and Ser-148, then by RseP (a site-2 protease). The N-terminal fragment is then degraded by primarily ClpX-ClpP in an ATP-dependent fashion. Sequential cleavage by DegS, RseP and ClpX-ClpP frees RpoE from RseA By similarity.By similarity

Interactioni

Subunit structurei

Interacts 1:1 with ECF RNA polymerase sigma-E (RpoE); this inhibits the interaction of sigma-E with the RNA polymerase catalytic core and leads to a decreased expression of sigma-E-regulated genes. Interacts with RseB with 1:1 stoichiometry. The liberated N-terminus forms a complex with SspB and RpoE By similarity.By similarity

Protein-protein interaction databases

STRINGi71421.HI0629.

Structurei

3D structure databases

ProteinModelPortaliP44791.
ModBaseiSearch...
MobiDBiSearch...

Topological domain

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9494CytoplasmicSequence AnalysisAdd
BLAST
Topological domaini118 – 19578PeriplasmicSequence AnalysisAdd
BLAST

Transmembrane

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei95 – 11723Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
BLAST

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili160 – 18122Sequence AnalysisAdd
BLAST

Domaini

The N-terminal cytosolic domain interacts with sigma-E. After degradation by RseP binds to SspB, targeting RseA for degradation by the ClpX-ClpP protease By similarity.By similarity
The C-terminal periplasmic domain interacts with RseB and is also the target for DegS.By similarity

Sequence similaritiesi

Belongs to the RseA family.Curated

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3073.
KOiK03597.
OMAiFILANFP.
OrthoDBiEOG6V7BSG.
PhylomeDBiP44791.

Family and domain databases

InterProiIPR005573. Anti-sigma_E_RseA_C.
IPR005572. Anti-sigma_E_RseA_N.
IPR026279. RseA.
[Graphical view]
PfamiPF03873. RseA_C. 1 hit.
PF03872. RseA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF016938. RseA. 1 hit.
SUPFAMiSSF89069. SSF89069. 1 hit.

Sequencei

Sequence statusi: Complete.

P44791-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQKEQLSAYM DGEQVETDLI DALLRDEELQ ASWHSFHTVR SVMRKESAVF
60 70 80 90 100
LGADFTAKMA DLIELEDVKK VDVIAVSQPE PEDAHNSVFM QKLKAFFAPM
110 120 130 140 150
TQVAVAAGVC LVAVLGVQSF NNKNDASNLP ETPVLQTLPF NNAVQEVSYN
160 170 180 190
APSKDTLTSD QLEKKSRRIG AMLQNYELQR RMHSDALDVS SSQVR
Length:195
Mass (Da):21,733
Last modified:November 1, 1995 - v1
Checksum:i9BA4C6AA8F91CA0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22289.1.
PIRiG64082.
RefSeqiNP_438789.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22289; AAC22289; HI_0629.
GeneIDi949684.
KEGGihin:HI0629.
PATRICi20189853. VBIHaeInf48452_0655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22289.1 .
PIRi G64082.
RefSeqi NP_438789.1. NC_000907.1.

3D structure databases

ProteinModelPortali P44791.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0629.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22289 ; AAC22289 ; HI_0629 .
GeneIDi 949684.
KEGGi hin:HI0629.
PATRICi 20189853. VBIHaeInf48452_0655.

Phylogenomic databases

eggNOGi COG3073.
KOi K03597.
OMAi FILANFP.
OrthoDBi EOG6V7BSG.
PhylomeDBi P44791.

Family and domain databases

InterProi IPR005573. Anti-sigma_E_RseA_C.
IPR005572. Anti-sigma_E_RseA_N.
IPR026279. RseA.
[Graphical view ]
Pfami PF03873. RseA_C. 1 hit.
PF03872. RseA_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF016938. RseA. 1 hit.
SUPFAMi SSF89069. SSF89069. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiRSEA_HAEIN
AccessioniPrimary (citable) accession number: P44791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: October 29, 2014
This is version 81 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3