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P44791

- RSEA_HAEIN

UniProt

P44791 - RSEA_HAEIN

Protein

Anti-sigma-E factor RseA

Gene

rseA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    An anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-E (RpoE). ECF sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein is first cut periplasmically (site-1 protease, S1P, DegS), then within the membrane itself (site-2 protease, S2P, RseP), while cytoplasmic proteases finish degrading the anti-sigma factor, liberating sigma-E By similarity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei148 – 1492Cleavage; by DegSBy similarity

    GO - Molecular functioni

    1. sigma factor antagonist activity Source: InterPro

    GO - Biological processi

    1. regulation of transcription, DNA-templated Source: UniProtKB-KW
    2. response to stress Source: InterPro
    3. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Biological processi

    Transcription, Transcription regulation

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Anti-sigma-E factor RseA
    Alternative name(s):
    Regulator of SigE
    Sigma-E anti-sigma factor RseA
    Sigma-E factor negative regulatory protein
    Gene namesi
    Name:rseA
    Synonyms:mclA
    Ordered Locus Names:HI_0629
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cell inner membrane By similarity; Single-pass type II membrane protein By similarity
    Note: Following cleavage by DegS the large fragment of the protein is still in the inner membrane and retains its anti-sigma-E activity.By similarity

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 195195Anti-sigma-E factor RseAPRO_0000097482Add
    BLAST

    Post-translational modificationi

    Sequentially cleaved by DegS (a site-1 protease) in its periplasmic domain between Val-147 and Ser-148, then by RseP (a site-2 protease). The N-terminal fragment is then degraded by primarily ClpX-ClpP in an ATP-dependent fashion. Sequential cleavage by DegS, RseP and ClpX-ClpP frees RpoE from RseA By similarity.By similarity

    Interactioni

    Subunit structurei

    Interacts 1:1 with ECF RNA polymerase sigma-E (RpoE); this inhibits the interaction of sigma-E with the RNA polymerase catalytic core and leads to a decreased expression of sigma-E-regulated genes. Interacts with RseB with 1:1 stoichiometry. The liberated N-terminus forms a complex with SspB and RpoE By similarity.By similarity

    Protein-protein interaction databases

    STRINGi71421.HI0629.

    Structurei

    3D structure databases

    ProteinModelPortaliP44791.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 9494CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini118 – 19578PeriplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei95 – 11723Helical; Signal-anchor for type II membrane proteinSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili160 – 18122Sequence AnalysisAdd
    BLAST

    Domaini

    The N-terminal cytosolic domain interacts with sigma-E. After degradation by RseP binds to SspB, targeting RseA for degradation by the ClpX-ClpP protease By similarity.By similarity
    The C-terminal periplasmic domain interacts with RseB and is also the target for DegS.By similarity

    Sequence similaritiesi

    Belongs to the RseA family.Curated

    Keywords - Domaini

    Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3073.
    KOiK03597.
    OMAiFILANFP.
    OrthoDBiEOG6V7BSG.
    PhylomeDBiP44791.

    Family and domain databases

    InterProiIPR005573. Anti-sigma_E_RseA_C.
    IPR005572. Anti-sigma_E_RseA_N.
    IPR026279. RseA.
    [Graphical view]
    PfamiPF03873. RseA_C. 1 hit.
    PF03872. RseA_N. 1 hit.
    [Graphical view]
    PIRSFiPIRSF016938. RseA. 1 hit.
    SUPFAMiSSF89069. SSF89069. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P44791-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQKEQLSAYM DGEQVETDLI DALLRDEELQ ASWHSFHTVR SVMRKESAVF    50
    LGADFTAKMA DLIELEDVKK VDVIAVSQPE PEDAHNSVFM QKLKAFFAPM 100
    TQVAVAAGVC LVAVLGVQSF NNKNDASNLP ETPVLQTLPF NNAVQEVSYN 150
    APSKDTLTSD QLEKKSRRIG AMLQNYELQR RMHSDALDVS SSQVR 195
    Length:195
    Mass (Da):21,733
    Last modified:November 1, 1995 - v1
    Checksum:i9BA4C6AA8F91CA0F
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22289.1.
    PIRiG64082.
    RefSeqiNP_438789.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22289; AAC22289; HI_0629.
    GeneIDi949684.
    KEGGihin:HI0629.
    PATRICi20189853. VBIHaeInf48452_0655.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22289.1 .
    PIRi G64082.
    RefSeqi NP_438789.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44791.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0629.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22289 ; AAC22289 ; HI_0629 .
    GeneIDi 949684.
    KEGGi hin:HI0629.
    PATRICi 20189853. VBIHaeInf48452_0655.

    Phylogenomic databases

    eggNOGi COG3073.
    KOi K03597.
    OMAi FILANFP.
    OrthoDBi EOG6V7BSG.
    PhylomeDBi P44791.

    Family and domain databases

    InterProi IPR005573. Anti-sigma_E_RseA_C.
    IPR005572. Anti-sigma_E_RseA_N.
    IPR026279. RseA.
    [Graphical view ]
    Pfami PF03873. RseA_C. 1 hit.
    PF03872. RseA_N. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF016938. RseA. 1 hit.
    SUPFAMi SSF89069. SSF89069. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiRSEA_HAEIN
    AccessioniPrimary (citable) accession number: P44791
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 80 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3