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P44791

- RSEA_HAEIN

UniProt

P44791 - RSEA_HAEIN

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Protein

Anti-sigma-E factor RseA

Gene
rseA, mclA, HI_0629
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

An anti-sigma factor for extracytoplasmic function (ECF) sigma factor sigma-E (RpoE). ECF sigma factors are held in an inactive form by an anti-sigma factor until released by regulated intramembrane proteolysis (RIP). RIP occurs when an extracytoplasmic signal triggers a concerted proteolytic cascade to transmit information and elicit cellular responses. The membrane-spanning regulatory substrate protein is first cut periplasmically (site-1 protease, S1P, DegS), then within the membrane itself (site-2 protease, S2P, RseP), while cytoplasmic proteases finish degrading the anti-sigma factor, liberating sigma-E By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei148 – 1492Cleavage; by DegS By similarity

GO - Molecular functioni

  1. sigma factor antagonist activity Source: InterPro

GO - Biological processi

  1. regulation of transcription, DNA-templated Source: UniProtKB-KW
  2. response to stress Source: InterPro
  3. transcription, DNA-templated Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

Transcription, Transcription regulation

Names & Taxonomyi

Protein namesi
Recommended name:
Anti-sigma-E factor RseA
Alternative name(s):
Regulator of SigE
Sigma-E anti-sigma factor RseA
Sigma-E factor negative regulatory protein
Gene namesi
Name:rseA
Synonyms:mclA
Ordered Locus Names:HI_0629
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

Subcellular locationi

Cell inner membrane; Single-pass type II membrane protein By similarity
Note: Following cleavage by DegS the large fragment of the protein is still in the inner membrane and retains its anti-sigma-E activity By similarity.

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 9494Cytoplasmic Reviewed predictionAdd
BLAST
Transmembranei95 – 11723Helical; Signal-anchor for type II membrane protein; Reviewed predictionAdd
BLAST
Topological domaini118 – 19578Periplasmic Reviewed predictionAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 195195Anti-sigma-E factor RseAPRO_0000097482Add
BLAST

Post-translational modificationi

Sequentially cleaved by DegS (a site-1 protease) in its periplasmic domain between Val-147 and Ser-148, then by RseP (a site-2 protease). The N-terminal fragment is then degraded by primarily ClpX-ClpP in an ATP-dependent fashion. Sequential cleavage by DegS, RseP and ClpX-ClpP frees RpoE from RseA By similarity.

Interactioni

Subunit structurei

Interacts 1:1 with ECF RNA polymerase sigma-E (RpoE); this inhibits the interaction of sigma-E with the RNA polymerase catalytic core and leads to a decreased expression of sigma-E-regulated genes. Interacts with RseB with 1:1 stoichiometry. The liberated N-terminus forms a complex with SspB and RpoE By similarity.

Protein-protein interaction databases

STRINGi71421.HI0629.

Structurei

3D structure databases

ProteinModelPortaliP44791.

Family & Domainsi

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili160 – 18122 Reviewed predictionAdd
BLAST

Domaini

The N-terminal cytosolic domain interacts with sigma-E. After degradation by RseP binds to SspB, targeting RseA for degradation by the ClpX-ClpP protease By similarity.
The C-terminal periplasmic domain interacts with RseB and is also the target for DegS By similarity.

Sequence similaritiesi

Belongs to the RseA family.

Keywords - Domaini

Coiled coil, Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3073.
KOiK03597.
OMAiFILANFP.
OrthoDBiEOG6V7BSG.
PhylomeDBiP44791.

Family and domain databases

InterProiIPR005573. Anti-sigma_E_RseA_C.
IPR005572. Anti-sigma_E_RseA_N.
IPR026279. RseA.
[Graphical view]
PfamiPF03873. RseA_C. 1 hit.
PF03872. RseA_N. 1 hit.
[Graphical view]
PIRSFiPIRSF016938. RseA. 1 hit.
SUPFAMiSSF89069. SSF89069. 1 hit.

Sequencei

Sequence statusi: Complete.

P44791-1 [UniParc]FASTAAdd to Basket

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MQKEQLSAYM DGEQVETDLI DALLRDEELQ ASWHSFHTVR SVMRKESAVF    50
LGADFTAKMA DLIELEDVKK VDVIAVSQPE PEDAHNSVFM QKLKAFFAPM 100
TQVAVAAGVC LVAVLGVQSF NNKNDASNLP ETPVLQTLPF NNAVQEVSYN 150
APSKDTLTSD QLEKKSRRIG AMLQNYELQR RMHSDALDVS SSQVR 195
Length:195
Mass (Da):21,733
Last modified:November 1, 1995 - v1
Checksum:i9BA4C6AA8F91CA0F
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22289.1.
PIRiG64082.
RefSeqiNP_438789.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22289; AAC22289; HI_0629.
GeneIDi949684.
KEGGihin:HI0629.
PATRICi20189853. VBIHaeInf48452_0655.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22289.1 .
PIRi G64082.
RefSeqi NP_438789.1. NC_000907.1.

3D structure databases

ProteinModelPortali P44791.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0629.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22289 ; AAC22289 ; HI_0629 .
GeneIDi 949684.
KEGGi hin:HI0629.
PATRICi 20189853. VBIHaeInf48452_0655.

Phylogenomic databases

eggNOGi COG3073.
KOi K03597.
OMAi FILANFP.
OrthoDBi EOG6V7BSG.
PhylomeDBi P44791.

Family and domain databases

InterProi IPR005573. Anti-sigma_E_RseA_C.
IPR005572. Anti-sigma_E_RseA_N.
IPR026279. RseA.
[Graphical view ]
Pfami PF03873. RseA_C. 1 hit.
PF03872. RseA_N. 1 hit.
[Graphical view ]
PIRSFi PIRSF016938. RseA. 1 hit.
SUPFAMi SSF89069. SSF89069. 1 hit.
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Entry informationi

Entry nameiRSEA_HAEIN
AccessioniPrimary (citable) accession number: P44791
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: July 9, 2014
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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