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Protein

Shikimate 5-dehydrogenase-like protein HI_0607

Gene

HI_0607

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

The physiological substrate is not known. Has much lower activity towards shikimate than AroE.1 Publication

Catalytic activityi

Shikimate + NAD(P)+ = 3-dehydroshikimate + NADH.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei67 – 671Proton acceptorSequence analysis
Binding sitei103 – 1031SubstrateCurated

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi126 – 1305NADPBy similarity
Nucleotide bindingi235 – 2395NADPBy similarity

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Ligandi

NADP

Enzyme and pathway databases

BRENDAi1.1.1.282. 2529.

Names & Taxonomyi

Protein namesi
Recommended name:
Shikimate 5-dehydrogenase-like protein HI_0607 (EC:1.1.1.-)
Short name:
SDH-L
Gene namesi
Ordered Locus Names:HI_0607
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi67 – 671K → A, H or N: Loss of activity. 1 Publication
Mutagenesisi103 – 1031D → A or N: Loss of activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Shikimate 5-dehydrogenase-like protein HI_0607PRO_0000136073Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0607.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi7 – 126Combined sources
Helixi19 – 3113Combined sources
Beta strandi35 – 406Combined sources
Helixi45 – 5511Combined sources
Beta strandi59 – 624Combined sources
Turni67 – 704Combined sources
Helixi71 – 733Combined sources
Beta strandi75 – 773Combined sources
Helixi79 – 824Combined sources
Turni83 – 853Combined sources
Beta strandi89 – 935Combined sources
Beta strandi96 – 1005Combined sources
Helixi102 – 11312Combined sources
Beta strandi122 – 1254Combined sources
Helixi131 – 14010Combined sources
Beta strandi146 – 1494Combined sources
Helixi153 – 16311Combined sources
Beta strandi166 – 1694Combined sources
Beta strandi177 – 1815Combined sources
Turni191 – 1944Combined sources
Helixi200 – 2056Combined sources
Beta strandi207 – 2115Combined sources
Beta strandi215 – 2184Combined sources
Helixi220 – 2278Combined sources
Beta strandi231 – 2333Combined sources
Helixi235 – 25117Combined sources
Helixi257 – 26812Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NPYX-ray1.75A/B/C/D1-271[»]
ProteinModelPortaliP44774.
SMRiP44774. Positions 1-270.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44774.

Family & Domainsi

Sequence similaritiesi

Belongs to the shikimate dehydrogenase family.Curated

Phylogenomic databases

eggNOGiENOG4105F9E. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiFVYKAFT.
OrthoDBiEOG6DZDX4.
PhylomeDBiP44774.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR013708. Shikimate_DH-bd_N.
[Graphical view]
PfamiPF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.

Sequencei

Sequence statusi: Complete.

P44774-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINKDTQLCM SLSGRPSNFG TTFHNYLYDK LGLNFIYKAF TTQDIEHAIK
60 70 80 90 100
GVRALGIRGC AVSMPFKETC MPFLDEIHPS AQAIESVNTI VNDNGFLRAY
110 120 130 140 150
NTDYIAIVKL IEKYHLNKNA KVIVHGSGGM AKAVVAAFKN SGFEKLKIYA
160 170 180 190 200
RNVKTGQYLA ALYGYAYINS LENQQADILV NVTSIGMKGG KEEMDLAFPK
210 220 230 240 250
AFIDNASVAF DVVAMPVETP FIRYAQARGK QTISGAAVIV LQAVEQFELY
260 270
THQRPSDELI AEAAAFARTK F
Length:271
Mass (Da):29,932
Last modified:November 1, 1995 - v1
Checksum:iA7A6AC9B9388394E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22266.1.
PIRiH64080.
RefSeqiNP_438765.1. NC_000907.1.
WP_005694543.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22266; AAC22266; HI_0607.
GeneIDi949659.
KEGGihin:HI0607.
PATRICi20189793. VBIHaeInf48452_0631.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22266.1.
PIRiH64080.
RefSeqiNP_438765.1. NC_000907.1.
WP_005694543.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NPYX-ray1.75A/B/C/D1-271[»]
ProteinModelPortaliP44774.
SMRiP44774. Positions 1-270.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0607.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22266; AAC22266; HI_0607.
GeneIDi949659.
KEGGihin:HI0607.
PATRICi20189793. VBIHaeInf48452_0631.

Phylogenomic databases

eggNOGiENOG4105F9E. Bacteria.
COG0169. LUCA.
KOiK00014.
OMAiFVYKAFT.
OrthoDBiEOG6DZDX4.
PhylomeDBiP44774.

Enzyme and pathway databases

BRENDAi1.1.1.282. 2529.

Miscellaneous databases

EvolutionaryTraceiP44774.

Family and domain databases

Gene3Di3.40.50.720. 1 hit.
InterProiIPR016040. NAD(P)-bd_dom.
IPR013708. Shikimate_DH-bd_N.
[Graphical view]
PfamiPF08501. Shikimate_dh_N. 1 hit.
[Graphical view]
SUPFAMiSSF51735. SSF51735. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Crystal structure of a novel shikimate dehydrogenase from Haemophilus influenzae."
    Singh S., Korolev S., Koroleva O., Zarembinski T., Collart F., Joachimiak A., Christendat D.
    J. Biol. Chem. 280:17101-17108(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.75 ANGSTROMS), FUNCTION, MUTAGENESIS OF LYS-67 AND ASP-103, SUBUNIT.

Entry informationi

Entry nameiY607_HAEIN
AccessioniPrimary (citable) accession number: P44774
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: December 9, 2015
This is version 107 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.