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Protein

Shikimate dehydrogenase-like protein HI_0607

Gene

sdhL

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

In vitro, is able to catalyze the NADP+-dependent oxidation of shikimate to 3-dehydroshikimate. However, has much lower activity than classical shikimate dehydrogenases AroE, indicating that shikimate may not be the biological substrate. Cannot utilize NAD+ instead of NADP+. Is not able to catalyze the oxidation of quinate.1 Publication

Catalytic activityi

Shikimate + NADP+ = 3-dehydroshikimate + NADPH.1 Publication

Kineticsi

kcat is 0.2 sec(-1).1 Publication
  1. KM=234 µM for shikimate1 Publication
  2. KM=234 µM for NADP+1 Publication

    pH dependencei

    Optimum pH is 8.8.1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Active sitei67Proton donor/acceptor1 Publication1
    Binding sitei103Substrate1 Publication1
    Binding sitei154NADPBy similarity1
    Binding sitei184NADPBy similarity1

    Regions

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Nucleotide bindingi126 – 130NADPBy similarity5

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionOxidoreductase
    LigandNADP

    Enzyme and pathway databases

    BRENDAi1.1.1.282. 2529.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Shikimate dehydrogenase-like protein HI_06071 Publication (EC:1.1.1.251 Publication)
    Short name:
    SDH-L1 Publication
    Gene namesi
    Name:sdhL1 Publication
    Ordered Locus Names:HI_0607
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    Proteomesi
    • UP000000579 Componenti: Chromosome

    Subcellular locationi

    GO - Cellular componenti

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi67K → A, H or N: Loss of activity. 1 Publication1
    Mutagenesisi103D → A or N: Loss of activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001360731 – 271Shikimate dehydrogenase-like protein HI_0607Add BLAST271

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    STRINGi71421.HI0607.

    Structurei

    Secondary structure

    1271
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details
    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Beta strandi7 – 12Combined sources6
    Helixi19 – 31Combined sources13
    Beta strandi35 – 40Combined sources6
    Helixi45 – 55Combined sources11
    Beta strandi59 – 62Combined sources4
    Turni67 – 70Combined sources4
    Helixi71 – 73Combined sources3
    Beta strandi75 – 77Combined sources3
    Helixi79 – 82Combined sources4
    Turni83 – 85Combined sources3
    Beta strandi89 – 93Combined sources5
    Beta strandi96 – 100Combined sources5
    Helixi102 – 113Combined sources12
    Beta strandi122 – 125Combined sources4
    Helixi131 – 140Combined sources10
    Beta strandi146 – 149Combined sources4
    Helixi153 – 163Combined sources11
    Beta strandi166 – 169Combined sources4
    Beta strandi177 – 181Combined sources5
    Turni191 – 194Combined sources4
    Helixi200 – 205Combined sources6
    Beta strandi207 – 211Combined sources5
    Beta strandi215 – 218Combined sources4
    Helixi220 – 227Combined sources8
    Beta strandi231 – 233Combined sources3
    Helixi235 – 251Combined sources17
    Helixi257 – 268Combined sources12

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1NPYX-ray1.75A/B/C/D1-271[»]
    ProteinModelPortaliP44774.
    SMRiP44774.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP44774.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the shikimate dehydrogenase-like family.2 Publications

    Phylogenomic databases

    eggNOGiENOG4105F9E. Bacteria.
    COG0169. LUCA.
    KOiK00014.
    OMAiMPFKESC.
    PhylomeDBiP44774.

    Family and domain databases

    InterProiView protein in InterPro
    IPR036291. NAD(P)-bd_dom_sf.
    IPR013708. Shikimate_DH-bd_N.
    PfamiView protein in Pfam
    PF08501. Shikimate_dh_N. 1 hit.
    SUPFAMiSSF51735. SSF51735. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P44774-1 [UniParc]FASTAAdd to basket

    « Hide

            10         20         30         40         50
    MINKDTQLCM SLSGRPSNFG TTFHNYLYDK LGLNFIYKAF TTQDIEHAIK
    60 70 80 90 100
    GVRALGIRGC AVSMPFKETC MPFLDEIHPS AQAIESVNTI VNDNGFLRAY
    110 120 130 140 150
    NTDYIAIVKL IEKYHLNKNA KVIVHGSGGM AKAVVAAFKN SGFEKLKIYA
    160 170 180 190 200
    RNVKTGQYLA ALYGYAYINS LENQQADILV NVTSIGMKGG KEEMDLAFPK
    210 220 230 240 250
    AFIDNASVAF DVVAMPVETP FIRYAQARGK QTISGAAVIV LQAVEQFELY
    260 270
    THQRPSDELI AEAAAFARTK F
    Length:271
    Mass (Da):29,932
    Last modified:November 1, 1995 - v1
    Checksum:iA7A6AC9B9388394E
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    L42023 Genomic DNA. Translation: AAC22266.1.
    PIRiH64080.
    RefSeqiNP_438765.1. NC_000907.1.
    WP_005694543.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22266; AAC22266; HI_0607.
    GeneIDi949659.
    KEGGihin:HI0607.
    PATRICifig|71421.8.peg.631.

    Similar proteinsi

    Entry informationi

    Entry nameiSHDHL_HAEIN
    AccessioniPrimary (citable) accession number: P44774
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 25, 2017
    This is version 115 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families