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Protein

tRNA (adenine(37)-N6)-methyltransferase

Gene

trmO

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Thr)(GGU). The methyl group of m6t6A37 appears to slightly improve the efficiency of the tRNA decoding ability. Binds to tRNA.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei92S-adenosyl-L-methionineBy similarity1
Binding sitei102S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(37)-N6)-methyltransferaseCurated (EC:2.1.1.-By similarity)
Alternative name(s):
tRNA (m6t6A37) methyltransferase
tRNA methyltransferase OBy similarity
Gene namesi
Name:trmOBy similarity
Synonyms:tsaABy similarity
Ordered Locus Names:HI_0510
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001556191 – 239tRNA (adenine(37)-N6)-methyltransferaseAdd BLAST239

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0510.

Structurei

Secondary structure

1239
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi4 – 6Combined sources3
Beta strandi10 – 13Combined sources4
Helixi19 – 21Combined sources3
Turni26 – 28Combined sources3
Beta strandi33 – 38Combined sources6
Turni40 – 42Combined sources3
Turni45 – 50Combined sources6
Helixi51 – 53Combined sources3
Beta strandi55 – 62Combined sources8
Helixi88 – 90Combined sources3
Beta strandi100 – 113Combined sources14
Beta strandi116 – 123Combined sources8
Beta strandi131 – 137Combined sources7
Helixi140 – 143Combined sources4
Beta strandi163 – 165Combined sources3
Helixi167 – 176Combined sources10
Turni177 – 179Combined sources3
Turni181 – 186Combined sources6
Beta strandi210 – 213Combined sources4
Beta strandi216 – 219Combined sources4
Beta strandi225 – 227Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XQBX-ray2.85A/B1-239[»]
ProteinModelPortaliP44740.
SMRiP44740.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44740.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini6 – 147TsaA-likePROSITE-ProRule annotationAdd BLAST142

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni23 – 25S-adenosyl-L-methionine bindingBy similarity3
Regioni64 – 65S-adenosyl-L-methionine bindingBy similarity2
Regioni127 – 130S-adenosyl-L-methionine bindingBy similarity4

Sequence similaritiesi

Contains 1 TsaA-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108R96. Bacteria.
COG1720. LUCA.
OMAiFASRSTH.
PhylomeDBiP44740.

Family and domain databases

CDDicd09281. UPF0066. 1 hit.
Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 1 hit.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDLTLSPIA IIHTPYKEKF SVPRQPNLVE DGVGIVELLP PYNSPEAVRG
60 70 80 90 100
LEQFSHLWLI FQFDQIQQGK WQPTVRPPRL GGNQRVGVFA SRATHRPNPL
110 120 130 140 150
GLSKVELRQV ECINGNIFLH LGAVDLVDGT PIFDIKPYIA YADSEPNAQS
160 170 180 190 200
SFAQEKLPVK LTVEFTEQAK SAVKKREEKR PHLSRFIRQV LEQDPRPAYQ
210 220 230
QGKPSDRIYG MSLYEFNVKW RIKAGTVNCV EVIEIEKDK
Length:239
Mass (Da):27,214
Last modified:November 1, 1995 - v1
Checksum:iE63FE78E7706106E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22168.1.
PIRiI64153.
RefSeqiNP_438668.1. NC_000907.1.
WP_005693665.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22168; AAC22168; HI_0510.
GeneIDi949577.
KEGGihin:HI0510.
PATRICi20189573. VBIHaeInf48452_0529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22168.1.
PIRiI64153.
RefSeqiNP_438668.1. NC_000907.1.
WP_005693665.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1XQBX-ray2.85A/B1-239[»]
ProteinModelPortaliP44740.
SMRiP44740.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22168; AAC22168; HI_0510.
GeneIDi949577.
KEGGihin:HI0510.
PATRICi20189573. VBIHaeInf48452_0529.

Phylogenomic databases

eggNOGiENOG4108R96. Bacteria.
COG1720. LUCA.
OMAiFASRSTH.
PhylomeDBiP44740.

Miscellaneous databases

EvolutionaryTraceiP44740.

Family and domain databases

CDDicd09281. UPF0066. 1 hit.
Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 1 hit.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTRMO_HAEIN
AccessioniPrimary (citable) accession number: P44740
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 102 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.