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Protein

tRNA (adenine(37)-N6)-methyltransferase

Gene

trmO

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

S-adenosyl-L-methionine-dependent methyltransferase responsible for the addition of the methyl group in the formation of N6-methyl-N6-threonylcarbamoyladenosine at position 37 (m6t6A37) of the tRNA anticodon loop of tRNA(Thr)(GGU). The methyl group of m6t6A37 appears to slightly improve the efficiency of the tRNA decoding ability. Binds to tRNA.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei92 – 921S-adenosyl-L-methionineBy similarity
Binding sitei102 – 1021S-adenosyl-L-methionine; via amide nitrogen and carbonyl oxygenBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

RNA-binding, S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (adenine(37)-N6)-methyltransferaseCurated (EC:2.1.1.-By similarity)
Alternative name(s):
tRNA (m6t6A37) methyltransferase
tRNA methyltransferase OBy similarity
Gene namesi
Name:trmOBy similarity
Synonyms:tsaABy similarity
Ordered Locus Names:HI_0510
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 239239tRNA (adenine(37)-N6)-methyltransferasePRO_0000155619Add
BLAST

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

STRINGi71421.HI0510.

Structurei

Secondary structure

1
239
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 63Combined sources
Beta strandi10 – 134Combined sources
Helixi19 – 213Combined sources
Turni26 – 283Combined sources
Beta strandi33 – 386Combined sources
Turni40 – 423Combined sources
Turni45 – 506Combined sources
Helixi51 – 533Combined sources
Beta strandi55 – 628Combined sources
Helixi88 – 903Combined sources
Beta strandi100 – 11314Combined sources
Beta strandi116 – 1238Combined sources
Beta strandi131 – 1377Combined sources
Helixi140 – 1434Combined sources
Beta strandi163 – 1653Combined sources
Helixi167 – 17610Combined sources
Turni177 – 1793Combined sources
Turni181 – 1866Combined sources
Beta strandi210 – 2134Combined sources
Beta strandi216 – 2194Combined sources
Beta strandi225 – 2273Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XQBX-ray2.85A/B1-239[»]
ProteinModelPortaliP44740.
SMRiP44740. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44740.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini6 – 147142TsaA-likePROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni23 – 253S-adenosyl-L-methionine bindingBy similarity
Regioni64 – 652S-adenosyl-L-methionine bindingBy similarity
Regioni127 – 1304S-adenosyl-L-methionine bindingBy similarity

Sequence similaritiesi

Contains 1 TsaA-like domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiENOG4108R96. Bacteria.
COG1720. LUCA.
OMAiFASRSTH.
OrthoDBiEOG60SCKQ.
PhylomeDBiP44740.

Family and domain databases

Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 1 hit.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44740-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNDLTLSPIA IIHTPYKEKF SVPRQPNLVE DGVGIVELLP PYNSPEAVRG
60 70 80 90 100
LEQFSHLWLI FQFDQIQQGK WQPTVRPPRL GGNQRVGVFA SRATHRPNPL
110 120 130 140 150
GLSKVELRQV ECINGNIFLH LGAVDLVDGT PIFDIKPYIA YADSEPNAQS
160 170 180 190 200
SFAQEKLPVK LTVEFTEQAK SAVKKREEKR PHLSRFIRQV LEQDPRPAYQ
210 220 230
QGKPSDRIYG MSLYEFNVKW RIKAGTVNCV EVIEIEKDK
Length:239
Mass (Da):27,214
Last modified:November 1, 1995 - v1
Checksum:iE63FE78E7706106E
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22168.1.
PIRiI64153.
RefSeqiNP_438668.1. NC_000907.1.
WP_005693665.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22168; AAC22168; HI_0510.
GeneIDi949577.
KEGGihin:HI0510.
PATRICi20189573. VBIHaeInf48452_0529.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22168.1.
PIRiI64153.
RefSeqiNP_438668.1. NC_000907.1.
WP_005693665.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1XQBX-ray2.85A/B1-239[»]
ProteinModelPortaliP44740.
SMRiP44740. Positions 1-231.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0510.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22168; AAC22168; HI_0510.
GeneIDi949577.
KEGGihin:HI0510.
PATRICi20189573. VBIHaeInf48452_0529.

Phylogenomic databases

eggNOGiENOG4108R96. Bacteria.
COG1720. LUCA.
OMAiFASRSTH.
OrthoDBiEOG60SCKQ.
PhylomeDBiP44740.

Miscellaneous databases

EvolutionaryTraceiP44740.

Family and domain databases

Gene3Di2.40.30.70. 1 hit.
InterProiIPR023370. TsaA-like.
IPR023368. UPF0066_cons_site.
[Graphical view]
PfamiPF01980. UPF0066. 1 hit.
[Graphical view]
SUPFAMiSSF118196. SSF118196. 1 hit.
TIGRFAMsiTIGR00104. tRNA_TsaA. 1 hit.
PROSITEiPS01318. TSAA_1. 1 hit.
PS51668. TSAA_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "X-ray structure of yaeb from Haemophilus influenzae. Northeast structural genomics research consortium (nesgc) target ir47."
    Northeast structural genomics consortium (NESG)
    Submitted (FEB-2009) to the PDB data bank
    Cited for: X-RAY CRYSTALLOGRAPHY (2.85 ANGSTROMS), SUBUNIT.

Entry informationi

Entry nameiTRMO_HAEIN
AccessioniPrimary (citable) accession number: P44740
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: March 16, 2016
This is version 99 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.