P44739 (MENA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
1,4-dihydroxy-2-naphthoate octaprenyltransferase
Short name=DHNA-octaprenyltransferase
EC=2.5.1.74

Gene names

Name:	menA
Ordered Locus Names:	HI_0509

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Conversion of 1,4-dihydroxy-2-naphthoate (DHNA) to dimethylmenaquinone (DMK). Attaches octaprenylpyrophosphate, a membrane-bound 40-carbon side chain to DHNA. The conversion of DHNA to DMK proceeds in three stages: the removal of the carboxyl group of DHNA as CO2, the attachment of the isoprenoid side chain, and a quinol-to-quinone oxidation, which is thought to be spontaneous By similarity.
Catalytic activity	An all-trans-polyprenyl diphosphate + 1,4-dihydroxy-2-naphthoate = a demethylmenaquinol + diphosphate + CO2.
Pathway	Cofactor biosynthesis; menaquinone biosynthesis; menaquinone-2 from chorismate: step 7/8.
Subcellular location	Cell inner membrane; Multi-pass membrane protein By similarity.
Sequence similarities	Belongs to the menA family.

Ontologies

Keywords
Biological process	Menaquinone biosynthesis
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Molecular function	Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	menaquinone biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	prenyltransferase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 308	308	1,4-dihydroxy-2-naphthoate octaprenyltransferase		PRO_0000096415
Regions
	Transmembrane	22 – 42	21	Helical; Potential
	Transmembrane	47 – 67	21	Helical; Potential
	Transmembrane	101 – 121	21	Helical; Potential
	Transmembrane	129 – 149	21	Helical; Potential
	Transmembrane	153 – 173	21	Helical; Potential
	Transmembrane	186 – 206	21	Helical; Potential
	Transmembrane	235 – 255	21	Helical; Potential
	Transmembrane	286 – 306	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P44739 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 7A873AFC8071AF6B	FASTA	308	33,346
	10 20 30 40 50 60 MRSKKMINEK LKMWWETARP KTLPLALASI FTGSALGYWA NPQGFNGLVM VLCLLTTILL 70 80 90 100 110 120 QVLSNFANDY GDHQKGSDTE ERIGPLRGIQ KGAISAKELK WGLILMVMAS FLSGSFLIGI 130 140 150 160 170 180 AYENLSDLFA FAGLGILAIV AAITYTVGVK PYGYMGLGDI SVLVFFGLLG VGGTYYLQTH 190 200 210 220 230 240 SIDSHIILPA IGSGLLASAV LNINNLRDIE QDAKAGKNTL AVRLGAYKGR VYHCILLSVA 250 260 270 280 290 300 ALCYLAFAVA TAISWTNYLF VLAMPLLAKH AIFVYCSQQP SELRPILAQM SMISLLINIL FSLGLLIG

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22167.1.
PIR	H64153.
RefSeq	NP_438667.2. NC_000907.1.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949923.
GenomeReviews	Gene locus HI_0509 in contig L42023_GR.
KEGG	hin:HI0509.
NMPDR	fig|71421.1.peg.480.
PATRIC	20189571. VBIHaeInf48452_0528.
TIGR	HI_0509.
Phylogenomic databases
HOGENOM	HBG313998.
OMA	ATLGTTY.
PhylomeDB	P44739.
ProtClustDB	PRK06080.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0509-MONOMER.
Family and domain databases
InterPro	IPR004657. DHNA_octaprenyltransferase. IPR000537. UbiA_prenyltransferase. [Graphical view]
KO	K02548.
Pfam	PF01040. UbiA. 1 hit. [Graphical view]
TIGRFAMs	TIGR00751. MenA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MENA_HAEIN

Accession

Primary (citable) accession number: P44739

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 78 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program