Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P44715 (PTFAH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Multiphosphoryl transfer protein

Short name=MTP
Alternative name(s):
Fructose PTS diphosphoryl transfer protein
Phosphotransferase FPr protein
Pseudo-HPr

Including the following 2 domains:

  1. Phosphocarrier protein HPr
    Short name=Protein H
  2. Fructose-specific phosphotransferase enzyme IIA component
    EC=2.7.1.-
    Alternative name(s):
    EIIA-Fru
    EIII-Fru
    PTS system fructose-specific EIIA component
Gene names
Name:fruB
Ordered Locus Names:HI_0448
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length499 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport By similarity.

Catalytic activity

Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.

Subcellular location

Cytoplasm By similarity.

Domain

In contrast to classical PTS systems, the fructose PTS has no requirement for HPr; FPr combines a IIA domain with two HPr domains. Ref.2

The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain. Ref.2

Sequence similarities

Contains 2 HPr domains.

Contains 1 PTS EIIA type-2 domain.

Ontologies

Keywords
   Biological processPhosphotransferase system
Sugar transport
Transport
   Cellular componentCytoplasm
   DomainRepeat
   Molecular functionKinase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processphosphoenolpyruvate-dependent sugar phosphotransferase system

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionkinase activity

Inferred from electronic annotation. Source: UniProtKB-KW

sugar:hydrogen symporter activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 499499Multiphosphoryl transfer protein
PRO_0000186520

Regions

Domain2 – 142141PTS EIIA type-2
Domain286 – 37691HPr 1
Domain410 – 49990HPr 2
Region155 – 285131M domain
Region378 – 40932Linker

Sites

Active site621Tele-phosphohistidine intermediate; for EIIA activity By similarity
Active site3001Pros-phosphohistidine intermediate; for HPr activity By similarity
Active site4241Pros-phosphohistidine intermediate; for HPr activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P44715 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D6DDC99884A58C63

FASTA49953,007
        10         20         30         40         50         60 
MLELSESNIH LNANAIDKQQ AIEMAVSALV QAGNVENGYL QGMLARELQT STFLGNGIAI 

        70         80         90        100        110        120 
PHGTLDTRLM VKKTGVQVFQ FPQGIEWGEG NIAYVVIGIA ARSDEHLSLL RQLTHVLSDE 

       130        140        150        160        170        180 
DTAAKLAKIT DVAEFCAILM GETIDPFEIP AANISLDVNT QSLLTLVAIN AGQLQVQSAV 

       190        200        210        220        230        240 
ENRFISEVIN NAALPLGKGL WVTDSVVGNV KNALAFSRAK TIFSHNGKAV KGVITVSAVG 

       250        260        270        280        290        300 
DQINPTLVRL LDDDVQTTLL NGNSTEILTA LLGSSSDVET QSVEGAVVGT FTIRNEHGLH 

       310        320        330        340        350        360 
ARPSANLVNE VKKFTSKITM QNLTRESEVV SAKSLMKIVA LGVTQGHRLR FVAEGEDAKQ 

       370        380        390        400        410        420 
AIESLGKAIA NGLGENVSAV PPSEPDTIEI MGDQIHTPAV TEDDNLPANA IEAVFVIKNE 

       430        440        450        460        470        480 
QGLHARPSAI LVNEVKKYNA SVAVQNLDRN SQLVSAKSLM KIVALGVVKG TRLRFVATGE 

       490 
EAQQAIDGIG AVIESGLGE 

« Hide

References

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22107.1.
PIRB64069.
RefSeqNP_438609.1. NC_000907.1.

3D structure databases

ProteinModelPortalP44715.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0448.

Protocols and materials databases

DNASU950651.
StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22107; AAC22107; HI_0448.
GeneID950651.
KEGGhin:HI0448.
PATRIC20189451. VBIHaeInf48452_0468.

Phylogenomic databases

eggNOGCOG1925.
KOK02768.
K11183.
OMAFPQGIAW.
OrthoDBEOG6XDGX2.
ProtClustDBPRK13779.

Family and domain databases

Gene3D3.30.1340.10. 2 hits.
3.40.930.10. 1 hit.
InterProIPR016258. Phosphotransferase_sys_FPr.
IPR016152. PTrfase/Anion_transptr.
IPR002178. PTS_EIIA_type-2_dom.
IPR001020. PTS_HPr_His_P_site.
IPR005698. PTS_HPr_prot.
IPR000032. PTS_HPr_prot-like.
IPR002114. PTS_HPr_Ser_P_site.
[Graphical view]
PfamPF00381. PTS-HPr. 2 hits.
PF00359. PTS_EIIA_2. 1 hit.
[Graphical view]
PIRSFPIRSF000690. Fruc_PTS_diPryltransf. 1 hit.
PRINTSPR00107. PHOSPHOCPHPR.
SUPFAMSSF55594. SSF55594. 2 hits.
SSF55804. SSF55804. 2 hits.
TIGRFAMsTIGR01003. PTS_HPr_family. 2 hits.
PROSITEPS51094. PTS_EIIA_TYPE_2. 1 hit.
PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
PS51350. PTS_HPR_DOM. 2 hits.
PS00369. PTS_HPR_HIS. 2 hits.
PS00589. PTS_HPR_SER. 2 hits.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePTFAH_HAEIN
AccessionPrimary (citable) accession number: P44715
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: April 16, 2014
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names