P44715 (PTFAH_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Multiphosphoryl transfer protein
Short name=MTP

Alternative name(s):
Fructose PTS diphosphoryl transfer protein
Phosphotransferase FPr protein
Pseudo-HPr

Including the following 2 domains:

1. Phosphocarrier protein HPr
   Short name=Protein H
2. Fructose-specific phosphotransferase enzyme IIA component
   EC=2.7.1.-
   Alternative name(s):
   EIIA-Fru
   EIII-Fru
   PTS system fructose-specific EIIA component

Gene names

Name:	fruB
Ordered Locus Names:	HI_0448

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	499 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport By similarity.
Catalytic activity	Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.
Subcellular location	Cytoplasm By similarity.
Domain	In contrast to classical PTS systems, the fructose PTS has no requirement for HPr; FPr combines a IIA domain with two HPr domains. Ref.2 The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain. Ref.2
Sequence similarities	Contains 2 HPr domains. Contains 1 PTS EIIA type-2 domain.

Ontologies

Keywords
Biological process	Phosphotransferase system Sugar transport Transport
Cellular component	Cytoplasm
Domain	Repeat
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	phosphoenolpyruvate-dependent sugar phosphotransferase system Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	kinase activity Inferred from electronic annotation. Source: UniProtKB-KW sugar:hydrogen symporter activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 499	499	Multiphosphoryl transfer protein		PRO_0000186520
Regions
	Domain	2 – 142	141	PTS EIIA type-2
	Domain	286 – 376	91	HPr 1
	Domain	410 – 499	90	HPr 2
	Region	155 – 285	131	M domain
	Region	378 – 409	32	Linker
Sites
	Active site	62	1	Tele-phosphohistidine intermediate; for EIIA activity By similarity
	Active site	300	1	Pros-phosphohistidine intermediate; for HPr activity By similarity
	Active site	424	1	Pros-phosphohistidine intermediate; for HPr activity By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44715 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: D6DDC99884A58C63	FASTA	499	53,007
	10 20 30 40 50 60 MLELSESNIH LNANAIDKQQ AIEMAVSALV QAGNVENGYL QGMLARELQT STFLGNGIAI 70 80 90 100 110 120 PHGTLDTRLM VKKTGVQVFQ FPQGIEWGEG NIAYVVIGIA ARSDEHLSLL RQLTHVLSDE 130 140 150 160 170 180 DTAAKLAKIT DVAEFCAILM GETIDPFEIP AANISLDVNT QSLLTLVAIN AGQLQVQSAV 190 200 210 220 230 240 ENRFISEVIN NAALPLGKGL WVTDSVVGNV KNALAFSRAK TIFSHNGKAV KGVITVSAVG 250 260 270 280 290 300 DQINPTLVRL LDDDVQTTLL NGNSTEILTA LLGSSSDVET QSVEGAVVGT FTIRNEHGLH 310 320 330 340 350 360 ARPSANLVNE VKKFTSKITM QNLTRESEVV SAKSLMKIVA LGVTQGHRLR FVAEGEDAKQ 370 380 390 400 410 420 AIESLGKAIA NGLGENVSAV PPSEPDTIEI MGDQIHTPAV TEDDNLPANA IEAVFVIKNE 430 440 450 460 470 480 QGLHARPSAI LVNEVKKYNA SVAVQNLDRN SQLVSAKSLM KIVALGVVKG TRLRFVATGE 490 EAQQAIDGIG AVIESGLGE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Novel PTS proteins revealed by bacterial genome sequencing: a unique fructose-specific phosphoryl transfer protein with two HPr-like domains in Haemophilus influenzae." Reizer J., Reizer A., Saier M.H. Jr. Res. Microbiol. 147:209-215(1996) [PubMed] [Europe PMC] [Abstract] Cited for: DOMAIN STRUCTURE.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22107.1.
PIR	B64069.
RefSeq	NP_438609.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44715.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0448.
Protocols and materials databases
DNASU	950651.
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22107; AAC22107; HI_0448.
GeneID	950651.
KEGG	hin:HI0448.
PATRIC	20189451. VBIHaeInf48452_0468.
Phylogenomic databases
eggNOG	COG1925.
KO	K02768. K11183.
OMA	AIPHGTL.
ProtClustDB	PRK13779.
Family and domain databases
Gene3D	3.30.1340.10. 2 hits. 3.40.930.10. 1 hit.
InterPro	IPR016258. Phosphotransferase_sys_FPr. IPR016152. PTrfase/Anion_transptr. IPR002178. PTS_EIIA_2. IPR001020. PTS_HPr_His_P_site. IPR005698. PTS_HPr_prot. IPR000032. PTS_HPr_prot-like. IPR002114. PTS_HPr_Ser_P_site. [Graphical view]
Pfam	PF00381. PTS-HPr. 2 hits. PF00359. PTS_EIIA_2. 1 hit. [Graphical view]
PIRSF	PIRSF000690. Fruc_PTS_diPryltransf. 1 hit.
PRINTS	PR00107. PHOSPHOCPHPR.
SUPFAM	SSF55594. HPr_protein. 2 hits. SSF55804. PTrfase/Anion_transptr. 2 hits.
TIGRFAMs	TIGR01003. PTS_HPr_family. 2 hits.
PROSITE	PS51094. PTS_EIIA_TYPE_2. 1 hit. PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit. PS51350. PTS_HPR_DOM. 2 hits. PS00369. PTS_HPR_HIS. 2 hits. PS00589. PTS_HPR_SER. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PTFAH_HAEIN

Accession

Primary (citable) accession number: P44715

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 106 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program