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P44715

- PTFAH_HAEIN

UniProt

P44715 - PTFAH_HAEIN

Protein

Multiphosphoryl transfer protein

Gene

fruB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport By similarity.By similarity

    Catalytic activityi

    Protein EIIA N(pi)-phospho-L-histidine + protein EIIB = protein EIIA + protein EIIB N(pi)-phospho-L-histidine/cysteine.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei62 – 621Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation
    Active sitei300 – 3001Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation
    Active sitei424 – 4241Pros-phosphohistidine intermediate; for HPr activityPROSITE-ProRule annotation

    GO - Molecular functioni

    1. kinase activity Source: UniProtKB-KW
    2. transporter activity Source: InterPro

    GO - Biological processi

    1. phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Biological processi

    Phosphotransferase system, Sugar transport, Transport

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Multiphosphoryl transfer protein
    Short name:
    MTP
    Alternative name(s):
    Fructose PTS diphosphoryl transfer protein
    Phosphotransferase FPr protein
    Pseudo-HPr
    Including the following 2 domains:
    Phosphocarrier protein HPr
    Short name:
    Protein H
    Fructose-specific phosphotransferase enzyme IIA component (EC:2.7.1.-)
    Alternative name(s):
    EIIA-Fru
    EIII-Fru
    PTS system fructose-specific EIIA component
    Gene namesi
    Name:fruB
    Ordered Locus Names:HI_0448
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 499499Multiphosphoryl transfer proteinPRO_0000186520Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0448.

    Structurei

    3D structure databases

    ProteinModelPortaliP44715.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini2 – 142141PTS EIIA type-2PROSITE-ProRule annotationAdd
    BLAST
    Domaini286 – 37691HPr 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini410 – 49990HPr 2PROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni155 – 285131M domainAdd
    BLAST
    Regioni378 – 40932LinkerAdd
    BLAST

    Domaini

    In contrast to classical PTS systems, the fructose PTS has no requirement for HPr; FPr combines a IIA domain with two HPr domains.1 Publication
    The EIIA domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB domain.1 PublicationPROSITE-ProRule annotation

    Sequence similaritiesi

    Contains 2 HPr domains.PROSITE-ProRule annotation
    Contains 1 PTS EIIA type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat

    Phylogenomic databases

    eggNOGiCOG1925.
    KOiK02768.
    K11183.
    OMAiFPQGIAW.
    OrthoDBiEOG6XDGX2.
    PhylomeDBiP44715.

    Family and domain databases

    Gene3Di3.30.1340.10. 2 hits.
    3.40.930.10. 1 hit.
    InterProiIPR000032. HPr_prot-like.
    IPR016258. Phosphotransferase_sys_FPr.
    IPR016152. PTrfase/Anion_transptr.
    IPR002178. PTS_EIIA_type-2_dom.
    IPR001020. PTS_HPr_His_P_site.
    IPR002114. PTS_HPr_Ser_P_site.
    [Graphical view]
    PfamiPF00381. PTS-HPr. 2 hits.
    PF00359. PTS_EIIA_2. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000690. Fruc_PTS_diPryltransf. 1 hit.
    PRINTSiPR00107. PHOSPHOCPHPR.
    SUPFAMiSSF55594. SSF55594. 2 hits.
    SSF55804. SSF55804. 2 hits.
    TIGRFAMsiTIGR01003. PTS_HPr_family. 2 hits.
    PROSITEiPS51094. PTS_EIIA_TYPE_2. 1 hit.
    PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
    PS51350. PTS_HPR_DOM. 2 hits.
    PS00369. PTS_HPR_HIS. 2 hits.
    PS00589. PTS_HPR_SER. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P44715-1 [UniParc]FASTAAdd to Basket

    « Hide

    MLELSESNIH LNANAIDKQQ AIEMAVSALV QAGNVENGYL QGMLARELQT    50
    STFLGNGIAI PHGTLDTRLM VKKTGVQVFQ FPQGIEWGEG NIAYVVIGIA 100
    ARSDEHLSLL RQLTHVLSDE DTAAKLAKIT DVAEFCAILM GETIDPFEIP 150
    AANISLDVNT QSLLTLVAIN AGQLQVQSAV ENRFISEVIN NAALPLGKGL 200
    WVTDSVVGNV KNALAFSRAK TIFSHNGKAV KGVITVSAVG DQINPTLVRL 250
    LDDDVQTTLL NGNSTEILTA LLGSSSDVET QSVEGAVVGT FTIRNEHGLH 300
    ARPSANLVNE VKKFTSKITM QNLTRESEVV SAKSLMKIVA LGVTQGHRLR 350
    FVAEGEDAKQ AIESLGKAIA NGLGENVSAV PPSEPDTIEI MGDQIHTPAV 400
    TEDDNLPANA IEAVFVIKNE QGLHARPSAI LVNEVKKYNA SVAVQNLDRN 450
    SQLVSAKSLM KIVALGVVKG TRLRFVATGE EAQQAIDGIG AVIESGLGE 499
    Length:499
    Mass (Da):53,007
    Last modified:November 1, 1995 - v1
    Checksum:iD6DDC99884A58C63
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22107.1.
    PIRiB64069.
    RefSeqiNP_438609.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22107; AAC22107; HI_0448.
    GeneIDi950651.
    KEGGihin:HI0448.
    PATRICi20189451. VBIHaeInf48452_0468.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22107.1 .
    PIRi B64069.
    RefSeqi NP_438609.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44715.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0448.

    Protocols and materials databases

    DNASUi 950651.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22107 ; AAC22107 ; HI_0448 .
    GeneIDi 950651.
    KEGGi hin:HI0448.
    PATRICi 20189451. VBIHaeInf48452_0468.

    Phylogenomic databases

    eggNOGi COG1925.
    KOi K02768.
    K11183.
    OMAi FPQGIAW.
    OrthoDBi EOG6XDGX2.
    PhylomeDBi P44715.

    Family and domain databases

    Gene3Di 3.30.1340.10. 2 hits.
    3.40.930.10. 1 hit.
    InterProi IPR000032. HPr_prot-like.
    IPR016258. Phosphotransferase_sys_FPr.
    IPR016152. PTrfase/Anion_transptr.
    IPR002178. PTS_EIIA_type-2_dom.
    IPR001020. PTS_HPr_His_P_site.
    IPR002114. PTS_HPr_Ser_P_site.
    [Graphical view ]
    Pfami PF00381. PTS-HPr. 2 hits.
    PF00359. PTS_EIIA_2. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000690. Fruc_PTS_diPryltransf. 1 hit.
    PRINTSi PR00107. PHOSPHOCPHPR.
    SUPFAMi SSF55594. SSF55594. 2 hits.
    SSF55804. SSF55804. 2 hits.
    TIGRFAMsi TIGR01003. PTS_HPr_family. 2 hits.
    PROSITEi PS51094. PTS_EIIA_TYPE_2. 1 hit.
    PS00372. PTS_EIIA_TYPE_2_HIS. 1 hit.
    PS51350. PTS_HPR_DOM. 2 hits.
    PS00369. PTS_HPR_HIS. 2 hits.
    PS00589. PTS_HPR_SER. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. "Novel PTS proteins revealed by bacterial genome sequencing: a unique fructose-specific phosphoryl transfer protein with two HPr-like domains in Haemophilus influenzae."
      Reizer J., Reizer A., Saier M.H. Jr.
      Res. Microbiol. 147:209-215(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: DOMAIN STRUCTURE.

    Entry informationi

    Entry nameiPTFAH_HAEIN
    AccessioniPrimary (citable) accession number: P44715
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 115 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3