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Reviewed, UniProtKB/Swiss-Prot P44714 (PTFBC_HAEIN)

Last modified February 9, 2010. Version 77. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

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Names and origin

Protein namesRecommended name:
    PTS system fructose-specific EIIBC component
Alternative name(s):
    EIIBC-Fru
Including the following 2 domains:
    1- Recommended name:
            Fructose-specific phosphotransferase enzyme IIB component
              EC=2.7.1.69
        Alternative name(s):
            PTS system fructose-specific EIIB component
            EIII-Fru
    2- Recommended name:
            Fructose permease IIC component
        Alternative name(s):
            PTS system fructose-specific EIIC component
Gene names
Name: fruA
Ordered Locus Names: HI0446
OrganismHaemophilus influenzae [Complete proteome] [HAMAP]
Taxonomic identifier727 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

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Protein attributes

Sequence length556 AA.
Sequence statusComplete.
Protein existenceInferred from homology.

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General annotation (Comments)

Function

The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This system is involved in fructose transport.

Catalytic activity

Protein EIIB N(pi)-phospho-L-histidine/cysteine + sugar = protein EIIB + sugar phosphate.

Subcellular location

Cell inner membrane; Multi-pass membrane protein By similarity.

Domain

The EIIB domain is phosphorylated by phospho-EIIA on a cysteinyl or histidyl residue, depending on the transported sugar. Then, it transfers the phosphoryl group to the sugar substrate concomitantly with the sugar uptake processed by the EIIC domain.

The EIIC domain forms the PTS system translocation channel and contains the specific substrate-binding site.

Sequence similarities

Contains 1 PTS EIIB type-2 domain.

Contains 1 PTS EIIC type-2 domain.

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Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 556556PTS system fructose-specific EIIBC component
PRO_0000186509

Regions

Transmembrane237 – 25721 Potential
Transmembrane275 – 29521 Potential
Transmembrane302 – 32221 Potential
Transmembrane324 – 34421 Potential
Transmembrane349 – 36921 Potential
Transmembrane390 – 41021 Potential
Transmembrane431 – 45121 Potential
Transmembrane468 – 48821 Potential
Transmembrane490 – 51021 Potential
Transmembrane529 – 54921 Potential
Domain106 – 20196PTS EIIB type-2
Domain224 – 556333PTS EIIC type-2

Sites

Active site1121Phosphocysteine intermediate; for EIIB activity By similarity

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Sequences

Sequence LengthMass (Da)Tools
P44714-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 31D8E77BFDBA7E42

FASTA55657,706
        10         20         30         40         50         60 
MKLFLTQSAN VGDVKAYLLH EVFRAAAQKA NVSIVGTPAE ADLVLVFGSV LPNNPDLVGK 

        70         80         90        100        110        120 
KVFIIGEAIA MISPEVTLAN ALANGADYVA PKSAVSFTGV SGVKNIVAVT ACPTGVAHTF 

       130        140        150        160        170        180 
MSAEAIEAYA KKQGWNVKVE TRGQVGAGNE ITVEEVAAAD LVFVAADIDV PLDKFKGKPM 

       190        200        210        220        230        240 
YRTSTGLALK KTEQEFDKAF KEAKIFDGGN NAGTKEESRE KKGVYKHLMT GVSHMLPLVV 

       250        260        270        280        290        300 
AGGLLIAISF MFSFNVIENT GVFQDLPNML INIGSGVAFK LMIAVFAGYV AFSIADRPGL 

       310        320        330        340        350        360 
AVGLIAGMLA SEAGAGILGG IIAGFLAGYV VKGLNVIIRL PASLTSLKPI LILPLLGSMI 

       370        380        390        400        410        420 
VGLTMIYLIN PPVAEIMKEL SNWLTSMGEV NAIVLGAIIG AMMCIDMGGP VNKAAYTFSV 

       430        440        450        460        470        480 
GLIASQVYTP MAAAMAAGMV PPIGMTVATW IARNKFTVSQ CDAGKASFVL GLCFISEGAL 

       490        500        510        520        530        540 
PFVAADPIRV IISSVIGGAV AGAISMGLNI TLQAPHGGLF VIPFVSEPLK YLGAIAIGAL 

       550 
STGVVYAIIK SKNNAE

« Hide

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Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L42023 Genomic DNA. Translation: AAC22105.1.
PIRI64068.
RefSeqNP_438607.1.

3D structure databases

SMRP44714. Positions 104-206.
ModBaseSearch...

Genome annotation databases

GeneID949670.
GenomeReviewsGene locus HI0446 in contig L42023_GR.
KEGGhin:HI0446.
NMPDRfig|71421.1.peg.420.
TIGRHI0446.

Phylogenomic databases

HOGENOMHBG704192.
OMAINHALLY.

Enzyme and pathway databases

BioCycHINF71421:HI_0446-MONOMER.
BRENDA2.7.1.69. 109.

Family and domain databases

InterProIPR013011. PTS_EIIB_2.
IPR003501. PTS_EIIB_2/3.
IPR003352. PTS_EIIC.
IPR013014. PTS_EIIC_2.
IPR003353. PTS_IIB_fruc.
IPR006327. PTS_IIC_fruc.
[Graphical view]
PfamPF02378. PTS_EIIC. 1 hit.
PF02302. PTS_IIB. 1 hit.
[Graphical view]
TIGRFAMsTIGR00829. FRU. 1 hit.
TIGR01427. PTS_IIC_fructo. 1 hit.
PROSITEPS51099. PTS_EIIB_TYPE_2. 1 hit.
PS51104. PTS_EIIC_TYPE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

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Entry information

Entry namePTFBC_HAEIN
AccessionPrimary (citable) accession number: P44714
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: February 9, 2010
This is version 77 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

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Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents