P44697 (THID_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
EC=2.7.1.49
EC=2.7.4.7

Alternative name(s):
Hydroxymethylpyrimidine kinase
Short name=HMP kinase
Hydroxymethylpyrimidine phosphate kinase
Short name=HMP-P kinase
Short name=HMP-phosphate kinase
Short name=HMPP kinase

Gene names

Name:	thiD
Ordered Locus Names:	HI_0416

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P By similarity.
Catalytic activity	ATP + 4-amino-5-hydroxymethyl-2-methylpyrimidine = ADP + 4-amino-5-phosphonooxymethyl-2-methylpyrimidine. ATP + 4-amino-2-methyl-5-phosphomethylpyrimidine = ADP + 4-amino-2-methyl-5-diphosphomethylpyrimidine.
Pathway	Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 2/3. Cofactor biosynthesis; thiamine diphosphate biosynthesis; 4-amino-2-methyl-5-diphosphomethylpyrimidine from 5-amino-1-(5-phospho-D-ribosyl)imidazole: step 3/3.
Sequence similarities	Belongs to the thiD family.

Ontologies

Keywords
Biological process	Thiamine biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	thiamine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW hydroxymethylpyrimidine kinase activity Inferred from electronic annotation. Source: EC phosphomethylpyrimidine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 269	269	Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase		PRO_0000192019
Sites
	Binding site	45	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44697 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 2B72DE9C39D1BEB3	FASTA	269	28,295
	10 20 30 40 50 60 MSNVKQVLTI AGSDSGGGAG IQADLKTFQM RGVFGTSAIT AVTAQNTLGV FDIHPIPLKT 70 80 90 100 110 120 IQAQLEAVKN DFQIASCKIG MLGNAEIIEC VADFLADKPF GTLVLDPVMI AKGGAPLLQR 130 140 150 160 170 180 QAVSALSQKL LPLADVITPN IPEAEALTGI AIVDDISIQQ AAKALQKQGA KNVIIKGGHS 190 200 210 220 230 240 LNSQSELCQD WVLLADGRHF TLQSPRFNTP HTHGTGCTFS ACLTAELAKG EPLQSAVKTA 250 260 KDFITAAISH PLNIGQGHGP TNHWAYSRL

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

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Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22074.1.
PIR	I64151.
RefSeq	NP_438578.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44697.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	949517.
GenomeReviews	Gene locus HI_0416 in contig L42023_GR.
KEGG	hin:HI0416.
NMPDR	fig|71421.1.peg.391.
PATRIC	20189385. VBIHaeInf48452_0436.
TIGR	HI_0416.
Phylogenomic databases
HOGENOM	HBG476673.
OMA	LNIGHGH.
PhylomeDB	P44697.
ProtClustDB	PRK06427.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0416-MONOMER.
Family and domain databases
InterPro	IPR013749. HMP-P_kinase-1. IPR004399. HMP-P_kinase-2. [Graphical view]
KO	K00941.
Pfam	PF08543. Phos_pyr_kin. 1 hit. [Graphical view]
TIGRFAMs	TIGR00097. HMP-P_kinase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

THID_HAEIN

Accession

Primary (citable) accession number: P44697

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 25, 2012
This is version 77 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program