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Protein

Methylated-DNA--protein-cysteine methyltransferase

Gene

ogt

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) and O4-methylthymine (O4-MeT) in DNA. Repairs the methylated nucleobase in DNA by stoichiometrically transferring the methyl group to a cysteine residue in the enzyme. This is a suicide reaction: the enzyme is irreversibly inactivated.UniRule annotation

Miscellaneous

This enzyme catalyzes only one turnover and therefore is not strictly catalytic. According to one definition, an enzyme is a biocatalyst that acts repeatedly and over many reaction cycles.

Catalytic activityi

DNA (containing 6-O-methylguanine) + protein L-cysteine = DNA (without 6-O-methylguanine) + protein S-methyl-L-cysteine.UniRule annotation
DNA (containing 4-O-methylthymine) + protein L-cysteine = DNA (without 4-O-methylthymine) + protein S-methyl-L-cysteine.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei130Nucleophile; methyl group acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionMethyltransferase, Transferase
Biological processDNA damage, DNA repair

Names & Taxonomyi

Protein namesi
Recommended name:
Methylated-DNA--protein-cysteine methyltransferaseUniRule annotation (EC:2.1.1.63UniRule annotation)
Alternative name(s):
6-O-methylguanine-DNA methyltransferaseUniRule annotation
Short name:
MGMTUniRule annotation
O-6-methylguanine-DNA-alkyltransferaseUniRule annotation
Gene namesi
Name:ogtUniRule annotation
Synonyms:dat1
Ordered Locus Names:HI_0402
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001393661 – 179Methylated-DNA--protein-cysteine methyltransferaseAdd BLAST179

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0402

Structurei

3D structure databases

ProteinModelPortaliP44687
SMRiP44687
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the MGMT family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105K85 Bacteria
COG0350 LUCA
KOiK00567
OMAiNPKSCRA

Family and domain databases

CDDicd06445 ATase, 1 hit
Gene3Di1.10.10.101 hit
HAMAPiMF_00772 OGT, 1 hit
InterProiView protein in InterPro
IPR001497 MethylDNA_cys_MeTrfase_AS
IPR014048 MethylDNA_cys_MeTrfase_DNA-bd
IPR036217 MethylDNA_cys_MeTrfase_DNAb
IPR008332 MethylG_MeTrfase_N
IPR023546 MGMT
IPR036631 MGMT_N_sf
IPR036388 WH-like_DNA-bd_sf
PfamiView protein in Pfam
PF01035 DNA_binding_1, 1 hit
PF02870 Methyltransf_1N, 1 hit
SUPFAMiSSF46767 SSF46767, 1 hit
SSF53155 SSF53155, 1 hit
TIGRFAMsiTIGR00589 ogt, 1 hit
PROSITEiView protein in PROSITE
PS00374 MGMT, 1 hit

Sequencei

Sequence statusi: Complete.

P44687-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALYYTYYP SPVGRLLILS DGESITHIDF EKEQYAPNPK WHKQDELPVF
60 70 80 90 100
QKVRLAFERY FNGEVECFSN IPLKPEGTAF QQAIWQALRE IDYGELSTYG
110 120 130 140 150
ELALRINNPK AVRAVGGAVG SNPISIIIPC HRILGKDRTL TGFGGGLEAK
160 170
RFLLQLEKIP YIDKGTENTK PRFFKKYHE
Length:179
Mass (Da):20,455
Last modified:May 10, 2004 - v2
Checksum:i9A83D5AFFC91D896
GO

Sequence cautioni

The sequence AAC22061 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA Translation: AAC22061.1 Different initiation.
PIRiG64065
RefSeqiNP_438564.1, NC_000907.1

Genome annotation databases

EnsemblBacteriaiAAC22061; AAC22061; HI_0402
GeneIDi949561
KEGGihin:HI0402
PATRICifig|71421.8.peg.421

Similar proteinsi

Entry informationi

Entry nameiOGT_HAEIN
AccessioniPrimary (citable) accession number: P44687
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: May 10, 2004
Last modified: February 28, 2018
This is version 114 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome