Reviewed, UniProtKB/Swiss-Prot P44684 (ADPP_HAEIN)

Names and origin

Protein names

Recommended name:
    ADP-ribose pyrophosphatase
    EC=3.6.1.13
Alternative name(s):
    ADP-ribose diphosphatase
    Adenosine diphosphoribose pyrophosphatase
      Short name=ADPR-PPase
    ADP-ribose phosphohydrolase
      Short name=ASPPase

Gene names

Name:	nudF
Ordered Locus Names:	HI0398

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	217 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Acts on ADP-mannose and ADP-glucose as well as ADP-ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process By similarity.
Catalytic activity	ADP-ribose + H2O = AMP + D-ribose 5-phosphate.
Cofactor	Binds 3 magnesium ions per subunit By similarity.
Sequence similarities	Belongs to the Nudix hydrolase family. NudF subfamily.

Ontologies

Keywords
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	ADP-ribose diphosphatase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 217	217	ADP-ribose pyrophosphatase		PRO_0000057045
Regions
	Motif	103 – 124	22	Nudix box
Sites
	Active site	168	1	Proton acceptor By similarity
	Metal binding	118	1	Magnesium 1 By similarity
	Metal binding	118	1	Magnesium 2 By similarity
	Metal binding	122	1	Magnesium 1 By similarity
	Metal binding	122	1	Magnesium 3 By similarity
	Metal binding	170	1	Magnesium 1 By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44684-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 6040146E71ABD400	FASTA	217	24,580
	10 20 30 40 50 60 MQFWRKQMSE IQHFSQQDIE ILGEQTLYEG FFTLKRIQFK HKLFAGGQSG VVTRELLIKG 70 80 90 100 110 120 AASAVIAYDP KEDSVILVEQ VRIGAAYHPE SHRSPWLLEL IAGMVEKGEK PEDVALRESE 130 140 150 160 170 180 EEAGIQVKNL THCLSVWDSP GGIVERIHLF AGEVDSAQAK GIHGLAEENE DIKVHVVKRE 190 200 210 QAYQWMCEGK IDNGIAVIGL QWLQLNYAQL QQSWKRS

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Studies on the ADP-ribose pyrophosphatase subfamily of the nudix hydrolases and tentative identification of trgB, a gene associated with tellurite resistance." Dunn C.A., O'Handley S.F., Frick D.N., Bessman M.J. J. Biol. Chem. 274:32318-32324(1999) [PubMed: 10542272] [Abstract] Cited for: CHARACTERIZATION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC22057.1.
PIR	B64151.
RefSeq	NP_438560.2.
3D structure databases
HSSP	HSSP built from PDB template 1KHZ based on UniProtKB P36651.
ModBase	Search...
Genome annotation databases
GeneID	949499.
GenomeReviews	Gene locus HI0398 in contig L42023_GR.
KEGG	hin:HI0398.
NMPDR	fig|71421.1.peg.373.
TIGR	HI0398.
Phylogenomic databases
HOGENOM	P44684.
OMA	P44684. YFPSPGG.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0398-MON.
BRENDA	3.6.1.13. 109.
Family and domain databases
InterPro	IPR004385. NDP_pyrophos. IPR000086. NUDIX_hydrolase_core. [Graphical view]
Gene3D	G3DSA:3.90.79.10. NUDIX_hydrolase. 1 hit.
Pfam	PF00293. NUDIX. 1 hit. [Graphical view]
TIGRFAMs	TIGR00052. NDP_pyrophos. 1 hit.
PROSITE	PS00893. NUDIX. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ADPP_HAEIN

Accession

Primary (citable) accession number: P44684

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	June 16, 2009
This is version 61 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)