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P44681

- YCHF_HAEIN

UniProt

P44681 - YCHF_HAEIN

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Protein

Ribosome-binding ATPase YchF

Gene
ychF, engD, HI_0393
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner By similarity. Does not hydrolyze GTP.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Magnesium By similarity
Metal bindingi36 – 361Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176ATP By similarity

GO - Molecular functioni

  1. ATPase activity Source: UniProtKB-HAMAP
  2. ATP binding Source: UniProtKB-HAMAP
  3. GTP binding Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. ribosomal large subunit binding Source: UniProtKB-HAMAP
  6. ribosome binding Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-3075-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosome-binding ATPase YchF
Gene namesi
Name:ychF
Synonyms:engD
Ordered Locus Names:HI_0393
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
ProteomesiUP000000579: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 363362Ribosome-binding ATPase YchFUniRule annotationPRO_0000201678Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0393.

Structurei

Secondary structure

1
363
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi4 – 85
Helixi15 – 2410
Beta strandi41 – 455
Helixi49 – 5810
Beta strandi61 – 644
Beta strandi67 – 726
Helixi80 – 834
Helixi84 – 863
Helixi90 – 956
Beta strandi98 – 1058
Helixi121 – 15131
Turni152 – 1543
Helixi156 – 17318
Helixi178 – 1803
Helixi185 – 1917
Helixi192 – 1943
Beta strandi202 – 2076
Beta strandi213 – 2153
Helixi217 – 22812
Beta strandi232 – 2365
Helixi238 – 2436
Helixi244 – 2463
Helixi253 – 2564
Helixi267 – 27610
Beta strandi279 – 2857
Beta strandi287 – 29610
Helixi301 – 3066
Helixi312 – 3154
Beta strandi318 – 3225
Helixi324 – 3296
Helixi333 – 3386
Beta strandi343 – 3453
Beta strandi356 – 3616

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1JALX-ray2.40A/B1-363[»]
ProteinModelPortaliP44681.
SMRiP44681. Positions 1-363.

Miscellaneous databases

EvolutionaryTraceiP44681.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 256254OBG-type GAdd
BLAST

Domaini

Composed of 3 distinct structural domains: the N-terminal domain, which has a mononucleotide binding fold typical for the P-loop NTPases, followed by a pronounced alpha-helical coiled coil domain and the C-terminal domain, which may be involved in RNA binding.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0012.
KOiK06942.
OMAiIPTTMEF.
OrthoDBiEOG6NSGJC.
PhylomeDBiP44681.

Family and domain databases

Gene3Di1.10.150.300. 1 hit.
3.10.20.30. 1 hit.
3.40.50.300. 2 hits.
HAMAPiMF_00944. YchF_OLA1_ATPase.
InterProiIPR004396. ATPase_YchF/OLA1.
IPR012675. Beta-grasp_dom.
IPR013029. DUF933.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
[Graphical view]
PfamiPF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
[Graphical view]
PIRSFiPIRSF006641. CHP00092. 1 hit.
PRINTSiPR00326. GTP1OBG.
SUPFAMiSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsiTIGR00092. TIGR00092. 1 hit.
PROSITEiPS51710. G_OBG. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44681-1 [UniParc]FASTAAdd to Basket

« Hide

MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NYPFCTIEPN TGVVPMPDPR    50
LDALAEIVKP ERILPTTMEF VDIAGLVAGA SKGEGLGNKF LANIRETDAI 100
GHVVRCFEND DIVHVAGKID PLDDIDTINT ELALADLDSC ERAIQRLQKR 150
AKGGDKEAKF ELSVMEKILP VLENAGMIRS VGLDKEELQA IKSYNFLTLK 200
PTMYIANVNE DGFENNPYLD RVREIAAKEG AVVVPVCAAI ESEIAELDDE 250
EKVEFLQDLG IEEPGLNRVI RAGYALLNLQ TYFTAGVKEV RAWTVSVGAT 300
APKAAAVIHT DFEKGFIRAE VIAYEDFIQF NGENGAKEAG KWRLEGKDYI 350
VQDGDVMHFR FNV 363
Length:363
Mass (Da):39,751
Last modified:January 23, 2007 - v2
Checksum:i08E00CBEF7D0F65E
GO

Mass spectrometryi

Molecular mass is 40011 Da from positions 2 - 363. Determined by MALDI. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22052.1.
PIRiI64150.
RefSeqiNP_438555.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22052; AAC22052; HI_0393.
GeneIDi949492.
KEGGihin:HI0393.
PATRICi20189337. VBIHaeInf48452_0412.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L42023 Genomic DNA. Translation: AAC22052.1 .
PIRi I64150.
RefSeqi NP_438555.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1JAL X-ray 2.40 A/B 1-363 [» ]
ProteinModelPortali P44681.
SMRi P44681. Positions 1-363.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 71421.HI0393.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC22052 ; AAC22052 ; HI_0393 .
GeneIDi 949492.
KEGGi hin:HI0393.
PATRICi 20189337. VBIHaeInf48452_0412.

Phylogenomic databases

eggNOGi COG0012.
KOi K06942.
OMAi IPTTMEF.
OrthoDBi EOG6NSGJC.
PhylomeDBi P44681.

Enzyme and pathway databases

BioCyci RETL1328306-WGS:GSTH-3075-MONOMER.

Miscellaneous databases

EvolutionaryTracei P44681.

Family and domain databases

Gene3Di 1.10.150.300. 1 hit.
3.10.20.30. 1 hit.
3.40.50.300. 2 hits.
HAMAPi MF_00944. YchF_OLA1_ATPase.
InterProi IPR004396. ATPase_YchF/OLA1.
IPR012675. Beta-grasp_dom.
IPR013029. DUF933.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
[Graphical view ]
Pfami PF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
[Graphical view ]
PIRSFi PIRSF006641. CHP00092. 1 hit.
PRINTSi PR00326. GTP1OBG.
SUPFAMi SSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsi TIGR00092. TIGR00092. 1 hit.
PROSITEi PS51710. G_OBG. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Two-dimensional map of the proteome of Haemophilus influenzae."
    Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M.
    Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  3. "Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid."
    Teplyakov A., Obmolova G., Chu S.Y., Toedt J., Eisenstein E., Howard A.J., Gilliland G.L.
    J. Bacteriol. 185:4031-4037(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), DOMAIN.
  4. "Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins."
    Koller-Eichhorn R., Marquardt T., Gail R., Wittinghofer A., Kostrewa D., Kutay U., Kambach C.
    J. Biol. Chem. 282:19928-19937(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, ATPASE ACTIVITY.

Entry informationi

Entry nameiYCHF_HAEIN
AccessioniPrimary (citable) accession number: P44681
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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