P44681 (YCHF_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ribosome-binding ATPase YchF

Gene names

Name:	ychF
Synonyms:	engD
Ordered Locus Names:	HI_0393

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner By similarity. Does not hydrolyze GTP. Ref.4
Domain	Composed of 3 distinct structural domains: the N-terminal domain, which has a mononucleotide binding fold typical for the P-loop NTPases, followed by a pronounced alpha-helical coiled coil domain and the C-terminal domain, which may be involved in RNA binding. Ref.3
Sequence similarities	Belongs to the GTP1/OBG family. YchF/OLA1 subfamily. Contains 1 G (guanine nucleotide-binding) domain.
Mass spectrometry	Molecular mass is 40011 Da from positions 2 - 363. Determined by MALDI. Ref.2

Ontologies

Keywords
Ligand	ATP-binding Nucleotide-binding
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW GTP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Initiator methionine

1

1

Removed By similarity

Chain

2 – 363

362

Ribosome-binding ATPase YchF HAMAP-Rule MF_00944

PRO_0000201678

Regions

Domain

4 – 109

106

G

Nucleotide binding

12 – 17

6

ATP By similarity

Secondary structure

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363

Helix Strand Turn

Details...

Beta strand

4 – 8

5

Helix

15 – 24

10

Beta strand

41 – 45

5

Helix

49 – 58

10

Beta strand

61 – 64

4

Beta strand

67 – 72

6

Helix

80 – 83

4

Helix

84 – 86

3

Helix

90 – 95

6

Beta strand

98 – 105

8

Helix

121 – 151

31

Turn

152 – 154

3

Helix

156 – 173

18

Helix

178 – 180

3

Helix

185 – 191

7

Helix

192 – 194

3

Beta strand

202 – 207

6

Beta strand

213 – 215

3

Helix

217 – 228

12

Beta strand

232 – 236

5

Helix

238 – 243

6

Helix

244 – 246

3

Helix

253 – 256

4

Helix

267 – 276

10

Beta strand

279 – 285

7

Beta strand

287 – 296

10

Helix

301 – 306

6

Helix

312 – 315

4

Beta strand

318 – 322

5

Helix

324 – 329

6

Helix

333 – 338

6

Beta strand

343 – 345

3

Beta strand

356 – 361

6

Sequences

	Sequence		Length	Mass (Da)
	P44681 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: 08E00CBEF7D0F65E	FASTA	363	39,751
	10 20 30 40 50 60 MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NYPFCTIEPN TGVVPMPDPR LDALAEIVKP 70 80 90 100 110 120 ERILPTTMEF VDIAGLVAGA SKGEGLGNKF LANIRETDAI GHVVRCFEND DIVHVAGKID 130 140 150 160 170 180 PLDDIDTINT ELALADLDSC ERAIQRLQKR AKGGDKEAKF ELSVMEKILP VLENAGMIRS 190 200 210 220 230 240 VGLDKEELQA IKSYNFLTLK PTMYIANVNE DGFENNPYLD RVREIAAKEG AVVVPVCAAI 250 260 270 280 290 300 ESEIAELDDE EKVEFLQDLG IEEPGLNRVI RAGYALLNLQ TYFTAGVKEV RAWTVSVGAT 310 320 330 340 350 360 APKAAAVIHT DFEKGFIRAE VIAYEDFIQF NGENGAKEAG KWRLEGKDYI VQDGDVMHFR FNV

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[3]	"Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid." Teplyakov A., Obmolova G., Chu S.Y., Toedt J., Eisenstein E., Howard A.J., Gilliland G.L. J. Bacteriol. 185:4031-4037(2003) [PubMed] [Europe PMC] [Abstract] Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), DOMAIN.
[4]	"Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins." Koller-Eichhorn R., Marquardt T., Gail R., Wittinghofer A., Kostrewa D., Kutay U., Kambach C. J. Biol. Chem. 282:19928-19937(2007) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION, ATPASE ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

L42023 Genomic DNA. Translation: AAC22052.1.

PIR

I64150.

RefSeq

NP_438555.1. NC_000907.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1JAL	X-ray	2.40	A/B	1-363	[»]

ProteinModelPortal

P44681.

SMR

P44681. Positions 1-363.

ModBase

Search...

Protein-protein interaction databases

STRING

71421.HI0393.

Proteomic databases

PRIDE

P44681.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

AAC22052; AAC22052; HI_0393.

GeneID

949492.

KEGG

hin:HI0393.

PATRIC

20189337. VBIHaeInf48452_0412.

Phylogenomic databases

eggNOG

COG0012.

KO

K06942.

OMA

DGDIMHF.

ProtClustDB

PRK09601.

Family and domain databases

Gene3D

1.10.150.300. 1 hit.
3.10.20.30. 1 hit.

HAMAP

MF_00944. YchF_OLA1_ATPase.

InterPro

IPR012675. Beta-grasp_dom.
IPR004396. CHP00092.
IPR013029. DUF933.
IPR006073. GTP_binding_domain.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
[Graphical view]

PANTHER

PTHR23305:SF4. PTHR23305:SF4. 1 hit.

Pfam

PF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
[Graphical view]

PIRSF

PIRSF006641. CHP00092. 1 hit.

PRINTS

PR00326. GTP1OBG.

SUPFAM

SSF81271. TGS-like. 1 hit.

TIGRFAMs

TIGR00092. TIGR00092. 1 hit.

ProtoNet

Search...

Other

EvolutionaryTrace

P44681.

Entry information

Entry name

YCHF_HAEIN

Accession

Primary (citable) accession number: P44681

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 101 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program