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P44681

- YCHF_HAEIN

UniProt

P44681 - YCHF_HAEIN

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Protein
Ribosome-binding ATPase YchF
Gene
ychF, engD, HI_0393
Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner By similarity. Does not hydrolyze GTP.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi16 – 161Magnesium By similarity
Metal bindingi36 – 361Magnesium By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi12 – 176ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. ATPase activity Source: UniProtKB-HAMAP
  3. GTP binding Source: InterPro
  4. metal ion binding Source: UniProtKB-KW
  5. ribosomal large subunit binding Source: UniProtKB-HAMAP
  6. ribosome binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

    Complete GO annotation...

    Keywords - Ligandi

    ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-3075-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ribosome-binding ATPase YchF
    Gene namesi
    Name:ychF
    Synonyms:engD
    Ordered Locus Names:HI_0393
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed By similarity
    Chaini2 – 363362Ribosome-binding ATPase YchFUniRule annotation
    PRO_0000201678Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0393.

    Structurei

    Secondary structure

    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi4 – 85
    Helixi15 – 2410
    Beta strandi41 – 455
    Helixi49 – 5810
    Beta strandi61 – 644
    Beta strandi67 – 726
    Helixi80 – 834
    Helixi84 – 863
    Helixi90 – 956
    Beta strandi98 – 1058
    Helixi121 – 15131
    Turni152 – 1543
    Helixi156 – 17318
    Helixi178 – 1803
    Helixi185 – 1917
    Helixi192 – 1943
    Beta strandi202 – 2076
    Beta strandi213 – 2153
    Helixi217 – 22812
    Beta strandi232 – 2365
    Helixi238 – 2436
    Helixi244 – 2463
    Helixi253 – 2564
    Helixi267 – 27610
    Beta strandi279 – 2857
    Beta strandi287 – 29610
    Helixi301 – 3066
    Helixi312 – 3154
    Beta strandi318 – 3225
    Helixi324 – 3296
    Helixi333 – 3386
    Beta strandi343 – 3453
    Beta strandi356 – 3616

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1JALX-ray2.40A/B1-363[»]
    ProteinModelPortaliP44681.
    SMRiP44681. Positions 1-363.

    Miscellaneous databases

    EvolutionaryTraceiP44681.

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini3 – 256254OBG-type G
    Add
    BLAST

    Domaini

    Composed of 3 distinct structural domains: the N-terminal domain, which has a mononucleotide binding fold typical for the P-loop NTPases, followed by a pronounced alpha-helical coiled coil domain and the C-terminal domain, which may be involved in RNA binding.1 Publication

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG0012.
    KOiK06942.
    OMAiIPTTMEF.
    OrthoDBiEOG6NSGJC.
    PhylomeDBiP44681.

    Family and domain databases

    Gene3Di1.10.150.300. 1 hit.
    3.10.20.30. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPiMF_00944. YchF_OLA1_ATPase.
    InterProiIPR004396. ATPase_YchF/OLA1.
    IPR012675. Beta-grasp_dom.
    IPR013029. DUF933.
    IPR006073. GTP_binding_domain.
    IPR027417. P-loop_NTPase.
    IPR012676. TGS-like.
    IPR023192. TGS-like_dom.
    [Graphical view]
    PfamiPF01926. MMR_HSR1. 1 hit.
    PF06071. YchF-GTPase_C. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006641. CHP00092. 1 hit.
    PRINTSiPR00326. GTP1OBG.
    SUPFAMiSSF52540. SSF52540. 1 hit.
    SSF81271. SSF81271. 1 hit.
    TIGRFAMsiTIGR00092. TIGR00092. 1 hit.
    PROSITEiPS51710. G_OBG. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P44681-1 [UniParc]FASTAAdd to Basket

    « Hide

    MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NYPFCTIEPN TGVVPMPDPR    50
    LDALAEIVKP ERILPTTMEF VDIAGLVAGA SKGEGLGNKF LANIRETDAI 100
    GHVVRCFEND DIVHVAGKID PLDDIDTINT ELALADLDSC ERAIQRLQKR 150
    AKGGDKEAKF ELSVMEKILP VLENAGMIRS VGLDKEELQA IKSYNFLTLK 200
    PTMYIANVNE DGFENNPYLD RVREIAAKEG AVVVPVCAAI ESEIAELDDE 250
    EKVEFLQDLG IEEPGLNRVI RAGYALLNLQ TYFTAGVKEV RAWTVSVGAT 300
    APKAAAVIHT DFEKGFIRAE VIAYEDFIQF NGENGAKEAG KWRLEGKDYI 350
    VQDGDVMHFR FNV 363
    Length:363
    Mass (Da):39,751
    Last modified:January 23, 2007 - v2
    Checksum:i08E00CBEF7D0F65E
    GO

    Mass spectrometryi

    Molecular mass is 40011 Da from positions 2 - 363. Determined by MALDI. 1 Publication

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22052.1.
    PIRiI64150.
    RefSeqiNP_438555.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22052; AAC22052; HI_0393.
    GeneIDi949492.
    KEGGihin:HI0393.
    PATRICi20189337. VBIHaeInf48452_0412.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22052.1 .
    PIRi I64150.
    RefSeqi NP_438555.1. NC_000907.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1JAL X-ray 2.40 A/B 1-363 [» ]
    ProteinModelPortali P44681.
    SMRi P44681. Positions 1-363.
    ModBasei Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0393.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22052 ; AAC22052 ; HI_0393 .
    GeneIDi 949492.
    KEGGi hin:HI0393.
    PATRICi 20189337. VBIHaeInf48452_0412.

    Phylogenomic databases

    eggNOGi COG0012.
    KOi K06942.
    OMAi IPTTMEF.
    OrthoDBi EOG6NSGJC.
    PhylomeDBi P44681.

    Enzyme and pathway databases

    BioCyci RETL1328306-WGS:GSTH-3075-MONOMER.

    Miscellaneous databases

    EvolutionaryTracei P44681.

    Family and domain databases

    Gene3Di 1.10.150.300. 1 hit.
    3.10.20.30. 1 hit.
    3.40.50.300. 2 hits.
    HAMAPi MF_00944. YchF_OLA1_ATPase.
    InterProi IPR004396. ATPase_YchF/OLA1.
    IPR012675. Beta-grasp_dom.
    IPR013029. DUF933.
    IPR006073. GTP_binding_domain.
    IPR027417. P-loop_NTPase.
    IPR012676. TGS-like.
    IPR023192. TGS-like_dom.
    [Graphical view ]
    Pfami PF01926. MMR_HSR1. 1 hit.
    PF06071. YchF-GTPase_C. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006641. CHP00092. 1 hit.
    PRINTSi PR00326. GTP1OBG.
    SUPFAMi SSF52540. SSF52540. 1 hit.
    SSF81271. SSF81271. 1 hit.
    TIGRFAMsi TIGR00092. TIGR00092. 1 hit.
    PROSITEi PS51710. G_OBG. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    « Hide 'large scale' publications
    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. "Two-dimensional map of the proteome of Haemophilus influenzae."
      Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M.
      Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    3. "Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid."
      Teplyakov A., Obmolova G., Chu S.Y., Toedt J., Eisenstein E., Howard A.J., Gilliland G.L.
      J. Bacteriol. 185:4031-4037(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), DOMAIN.
    4. "Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins."
      Koller-Eichhorn R., Marquardt T., Gail R., Wittinghofer A., Kostrewa D., Kutay U., Kambach C.
      J. Biol. Chem. 282:19928-19937(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, ATPASE ACTIVITY.

    Entry informationi

    Entry nameiYCHF_HAEIN
    AccessioniPrimary (citable) accession number: P44681
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: January 23, 2007
    Last modified: July 9, 2014
    This is version 111 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3

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