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P44681 (YCHF_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 111. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ribosome-binding ATPase YchF
Gene names
Name:ychF
Synonyms:engD
Ordered Locus Names:HI_0393
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length363 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

ATPase that binds to both the 70S ribosome and the 50S ribosomal subunit in a nucleotide-independent manner By similarity. Does not hydrolyze GTP. Ref.4

Cofactor

Magnesium By similarity. HAMAP-Rule MF_00944

Domain

Composed of 3 distinct structural domains: the N-terminal domain, which has a mononucleotide binding fold typical for the P-loop NTPases, followed by a pronounced alpha-helical coiled coil domain and the C-terminal domain, which may be involved in RNA binding. Ref.3

Sequence similarities

Belongs to the TRAFAC class OBG-HflX-like GTPase superfamily. OBG GTPase family. YchF/OLA1 subfamily.

Contains 1 OBG-type G (guanine nucleotide-binding) domain.

Mass spectrometry

Molecular mass is 40011 Da from positions 2 - 363. Determined by MALDI. Ref.2

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 363362Ribosome-binding ATPase YchF HAMAP-Rule MF_00944
PRO_0000201678

Regions

Domain3 – 256254OBG-type G
Nucleotide binding12 – 176ATP By similarity

Sites

Metal binding161Magnesium By similarity
Metal binding361Magnesium By similarity

Secondary structure

............................................................... 363
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P44681 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: 08E00CBEF7D0F65E

FASTA36339,751
        10         20         30         40         50         60 
MGFKCGIVGL PNVGKSTLFN ALTKAGIEAA NYPFCTIEPN TGVVPMPDPR LDALAEIVKP 

        70         80         90        100        110        120 
ERILPTTMEF VDIAGLVAGA SKGEGLGNKF LANIRETDAI GHVVRCFEND DIVHVAGKID 

       130        140        150        160        170        180 
PLDDIDTINT ELALADLDSC ERAIQRLQKR AKGGDKEAKF ELSVMEKILP VLENAGMIRS 

       190        200        210        220        230        240 
VGLDKEELQA IKSYNFLTLK PTMYIANVNE DGFENNPYLD RVREIAAKEG AVVVPVCAAI 

       250        260        270        280        290        300 
ESEIAELDDE EKVEFLQDLG IEEPGLNRVI RAGYALLNLQ TYFTAGVKEV RAWTVSVGAT 

       310        320        330        340        350        360 
APKAAAVIHT DFEKGFIRAE VIAYEDFIQF NGENGAKEAG KWRLEGKDYI VQDGDVMHFR 


FNV 

« Hide

References

« Hide 'large scale' references
[1]"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd."
Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. expand/collapse author list , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C.
Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]"Two-dimensional map of the proteome of Haemophilus influenzae."
Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M.
Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[3]"Crystal structure of the YchF protein reveals binding sites for GTP and nucleic acid."
Teplyakov A., Obmolova G., Chu S.Y., Toedt J., Eisenstein E., Howard A.J., Gilliland G.L.
J. Bacteriol. 185:4031-4037(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (2.4 ANGSTROMS), DOMAIN.
[4]"Human OLA1 defines an ATPase subfamily in the Obg family of GTP-binding proteins."
Koller-Eichhorn R., Marquardt T., Gail R., Wittinghofer A., Kostrewa D., Kutay U., Kambach C.
J. Biol. Chem. 282:19928-19937(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, ATPASE ACTIVITY.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC22052.1.
PIRI64150.
RefSeqNP_438555.1. NC_000907.1.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1JALX-ray2.40A/B1-363[»]
ProteinModelPortalP44681.
SMRP44681. Positions 1-363.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC22052; AAC22052; HI_0393.
GeneID949492.
KEGGhin:HI0393.
PATRIC20189337. VBIHaeInf48452_0412.

Phylogenomic databases

eggNOGCOG0012.
KOK06942.
OMAIPTTMEF.
OrthoDBEOG6NSGJC.
PhylomeDBP44681.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-3075-MONOMER.

Family and domain databases

Gene3D1.10.150.300. 1 hit.
3.10.20.30. 1 hit.
3.40.50.300. 2 hits.
HAMAPMF_00944. YchF_OLA1_ATPase.
InterProIPR004396. ATPase_YchF/OLA1.
IPR012675. Beta-grasp_dom.
IPR013029. DUF933.
IPR006073. GTP_binding_domain.
IPR027417. P-loop_NTPase.
IPR012676. TGS-like.
IPR023192. TGS-like_dom.
[Graphical view]
PfamPF01926. MMR_HSR1. 1 hit.
PF06071. YchF-GTPase_C. 1 hit.
[Graphical view]
PIRSFPIRSF006641. CHP00092. 1 hit.
PRINTSPR00326. GTP1OBG.
SUPFAMSSF52540. SSF52540. 1 hit.
SSF81271. SSF81271. 1 hit.
TIGRFAMsTIGR00092. TIGR00092. 1 hit.
PROSITEPS51710. G_OBG. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP44681.

Entry information

Entry nameYCHF_HAEIN
AccessionPrimary (citable) accession number: P44681
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 111 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names