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Protein

Periplasmic nitrate reductase, electron transfer subunit

Gene

napB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Electron transfer subunit of the periplasmic nitrate reductase complex NapAB. Receives electrons from the membrane-anchored tetraheme c-type NapC protein and transfers these to NapA subunit, thus allowing electron flow between membrane and periplasm. Essential for periplasmic nitrate reduction with nitrate as the terminal electron acceptor.1 Publication

Redox potential

E0 are -25 mV and -175 mV.1 Publication

Manual assertion based on experiment ini

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi70Iron (heme C 1 axial ligand); via tele nitrogen1
Binding sitei84Heme C 1 (covalent)1 Publication1
Binding sitei87Heme C 1 (covalent)1 Publication1
Metal bindingi88Iron (heme C 1 axial ligand); via tele nitrogen1
Metal bindingi105Iron (heme C 2 axial ligand); via tele nitrogen1
Binding sitei124Heme C 2 (covalent)1 Publication1
Binding sitei127Heme C 2 (covalent)1 Publication1
Metal bindingi128Iron (heme C 2 axial ligand); via tele nitrogen1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Electron transport, Transport

Keywords - Ligandi

Heme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Periplasmic nitrate reductase, electron transfer subunit
Alternative name(s):
Diheme cytochrome c NapB
Gene namesi
Name:napB
Ordered Locus Names:HI_0347
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 261 PublicationAdd BLAST26
ChainiPRO_000000658927 – 150Periplasmic nitrate reductase, electron transfer subunitAdd BLAST124

Post-translational modificationi

Binds 2 heme C groups per subunit.

Interactioni

Subunit structurei

Component of the periplasmic nitrate reductase NapAB complex composed of NapA and NapB.By similarity

Protein-protein interaction databases

STRINGi71421.HI0347.

Structurei

Secondary structure

1150
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi85 – 88Combined sources4
Turni90 – 92Combined sources3
Helixi93 – 96Combined sources4
Helixi103 – 105Combined sources3
Helixi124 – 126Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JNIX-ray1.25A28-150[»]
ProteinModelPortaliP44654.
SMRiP44654.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44654.

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi115 – 118Poly-Ser4

Sequence similaritiesi

Belongs to the NapB family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4105IYZ. Bacteria.
COG3043. LUCA.
KOiK02568.
OMAiPRVPESH.
PhylomeDBiP44654.

Family and domain databases

Gene3Di4.10.1020.10. 1 hit.
InterProiIPR011031. Multihaem_cyt.
IPR005591. NapB.
IPR027406. NapB_heme-bd.
[Graphical view]
PfamiPF03892. NapB. 1 hit.
[Graphical view]
PIRSFiPIRSF006105. NapB. 1 hit.
SUPFAMiSSF48695. SSF48695. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P44654-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MINMTKQVSK ILAGLFTALF AGSLMASDAP AVGKDLTQAA ENIPPAFHNA
60 70 80 90 100
PRQGELPALN YVNQPPMVPH SVANYQVTKN VNQCLNCHSP ENSRLSGATR
110 120 130 140 150
ISPTHFMDRD GKVGSSSSPR RYFCLQCHVS QANVDPIVPN DFKPMKGYGN
Length:150
Mass (Da):16,255
Last modified:November 1, 1995 - v1
Checksum:iC5E98C4E1B29E01F
GO

Mass spectrometryi

Molecular mass is 14748.68 Da from positions 27 - 150. Determined by ESI. 1 Publication

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22008.1.
PIRiC64149.
RefSeqiNP_438511.1. NC_000907.1.
WP_005691774.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC22008; AAC22008; HI_0347.
GeneIDi949780.
KEGGihin:HI0347.
PATRICi20189241. VBIHaeInf48452_0366.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC22008.1.
PIRiC64149.
RefSeqiNP_438511.1. NC_000907.1.
WP_005691774.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1JNIX-ray1.25A28-150[»]
ProteinModelPortaliP44654.
SMRiP44654.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0347.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC22008; AAC22008; HI_0347.
GeneIDi949780.
KEGGihin:HI0347.
PATRICi20189241. VBIHaeInf48452_0366.

Phylogenomic databases

eggNOGiENOG4105IYZ. Bacteria.
COG3043. LUCA.
KOiK02568.
OMAiPRVPESH.
PhylomeDBiP44654.

Miscellaneous databases

EvolutionaryTraceiP44654.

Family and domain databases

Gene3Di4.10.1020.10. 1 hit.
InterProiIPR011031. Multihaem_cyt.
IPR005591. NapB.
IPR027406. NapB_heme-bd.
[Graphical view]
PfamiPF03892. NapB. 1 hit.
[Graphical view]
PIRSFiPIRSF006105. NapB. 1 hit.
SUPFAMiSSF48695. SSF48695. 1 hit.
PROSITEiPS51008. MULTIHEME_CYTC. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNAPB_HAEIN
AccessioniPrimary (citable) accession number: P44654
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.