P44648 (TRMB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA (guanine-N(7)-)-methyltransferase
EC=2.1.1.33

Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferase
tRNA(m7G46)-methyltransferase

Gene names

Name:	trmB
Ordered Locus Names:	HI_0340

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	246 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA By similarity. HAMAP-Rule MF_01057
Catalytic activity	S-adenosyl-L-methionine + guanine46 in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine46 in tRNA. HAMAP-Rule MF_01057
Pathway	tRNA modification; N(7)-methylguanine-tRNA biosynthesis. HAMAP-Rule MF_01057
Sequence similarities	Belongs to the methyltransferase superfamily. TrmB family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	RNA (guanine-N7)-methylation Inferred from electronic annotation. Source: GOC
Molecular_function	tRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 246	246	tRNA (guanine-N(7)-)-methyltransferase HAMAP-Rule MF_01057		PRO_0000171333
Regions
	Region	225 – 228	4	Substrate binding Potential
Sites
	Binding site	77	1	S-adenosyl-L-methionine By similarity
	Binding site	102	1	S-adenosyl-L-methionine By similarity
	Binding site	129	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
	Binding site	156	1	Substrate By similarity
	Binding site	188	1	Substrate Potential

Sequences

	Sequence		Length	Mass (Da)
	P44648 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 1FF1DFD12FEC729D	FASTA	246	28,053
	10 20 30 40 50 60 MTQTFADQKR KTVETAEFTE DGRYKRKVRS FVLRTGRLSE FQRNMMNDNW GTLGLDYQTE 70 80 90 100 110 120 PFDFAKIYGN DNPVVLEIGF GMGKSLVDMA FANPDKNYLG IEVHTPGVGA CIAYAVEKGV 130 140 150 160 170 180 TNLRVICHDA TEILRDSIAD GALGGLQLFF PDPWHKAKHH KRRIVQPHFV TQVIQKLGEN 190 200 210 220 230 240 GFIHMATDWE NYAEQMLEVL SANTDLVNTS KNGDYIPRPD FRPLTKFEAR GYKLGHGVWD LYFVKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC22002.1.
PIR	F64148.
RefSeq	NP_438504.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44648.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0340.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC22002; AAC22002; HI_0340.
GeneID	949446.
KEGG	hin:HI0340.
PATRIC	20189223. VBIHaeInf48452_0357.
Phylogenomic databases
eggNOG	COG0220.
KO	K03439.
OMA	CEVHEPG.
ProtClustDB	PRK00121.
Enzyme and pathway databases
UniPathway	UPA00989.
Family and domain databases
HAMAP	MF_01057. tRNA_methyltr_TrmB.
InterPro	IPR003358. tRNA_(Gua-N-7)_MeTrfase. [Graphical view]
Pfam	PF02390. Methyltransf_4. 1 hit. [Graphical view]
TIGRFAMs	TIGR00091. TIGR00091. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TRMB_HAEIN

Accession

Primary (citable) accession number: P44648

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 88 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program