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P44648

- TRMB_HAEIN

UniProt

P44648 - TRMB_HAEIN

Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 97 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    • Comment

    Functioni

    Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

    Catalytic activityi

    S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei77 – 771S-adenosyl-L-methionineUniRule annotation
    Binding sitei102 – 1021S-adenosyl-L-methionineUniRule annotation
    Binding sitei129 – 1291S-adenosyl-L-methionineUniRule annotation
    Active sitei152 – 1521By similarity
    Binding sitei152 – 1521S-adenosyl-L-methionineUniRule annotation
    Binding sitei156 – 1561SubstrateUniRule annotation
    Binding sitei188 – 1881SubstrateUniRule annotation

    GO - Molecular functioni

    1. tRNA (guanine-N7-)-methyltransferase activity Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    tRNA processing

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Enzyme and pathway databases

    UniPathwayiUPA00989.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
    Alternative name(s):
    tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
    tRNA(m7G46)-methyltransferaseUniRule annotation
    Gene namesi
    Name:trmBUniRule annotation
    Ordered Locus Names:HI_0340
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 246246tRNA (guanine-N(7)-)-methyltransferasePRO_0000171333Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0340.

    Structurei

    3D structure databases

    ProteinModelPortaliP44648.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni225 – 2284Substrate bindingUniRule annotation

    Sequence similaritiesi

    Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0220.
    KOiK03439.
    OMAiHSTLEMA.
    OrthoDBiEOG6K6VBC.
    PhylomeDBiP44648.

    Family and domain databases

    Gene3Di3.40.50.150. 1 hit.
    HAMAPiMF_01057. tRNA_methyltr_TrmB.
    InterProiIPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view]
    PfamiPF02390. Methyltransf_4. 1 hit.
    [Graphical view]
    SUPFAMiSSF53335. SSF53335. 1 hit.
    TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
    PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P44648-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTQTFADQKR KTVETAEFTE DGRYKRKVRS FVLRTGRLSE FQRNMMNDNW    50
    GTLGLDYQTE PFDFAKIYGN DNPVVLEIGF GMGKSLVDMA FANPDKNYLG 100
    IEVHTPGVGA CIAYAVEKGV TNLRVICHDA TEILRDSIAD GALGGLQLFF 150
    PDPWHKAKHH KRRIVQPHFV TQVIQKLGEN GFIHMATDWE NYAEQMLEVL 200
    SANTDLVNTS KNGDYIPRPD FRPLTKFEAR GYKLGHGVWD LYFVKK 246
    Length:246
    Mass (Da):28,053
    Last modified:November 1, 1995 - v1
    Checksum:i1FF1DFD12FEC729D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22002.1.
    PIRiF64148.
    RefSeqiNP_438504.1. NC_000907.1.
    WP_005694335.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC22002; AAC22002; HI_0340.
    GeneIDi949446.
    KEGGihin:HI0340.
    PATRICi20189223. VBIHaeInf48452_0357.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC22002.1 .
    PIRi F64148.
    RefSeqi NP_438504.1. NC_000907.1.
    WP_005694335.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44648.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0340.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC22002 ; AAC22002 ; HI_0340 .
    GeneIDi 949446.
    KEGGi hin:HI0340.
    PATRICi 20189223. VBIHaeInf48452_0357.

    Phylogenomic databases

    eggNOGi COG0220.
    KOi K03439.
    OMAi HSTLEMA.
    OrthoDBi EOG6K6VBC.
    PhylomeDBi P44648.

    Enzyme and pathway databases

    UniPathwayi UPA00989 .

    Family and domain databases

    Gene3Di 3.40.50.150. 1 hit.
    HAMAPi MF_01057. tRNA_methyltr_TrmB.
    InterProi IPR029063. SAM-dependent_MTases-like.
    IPR003358. tRNA_(Gua-N-7)_MeTrfase.
    [Graphical view ]
    Pfami PF02390. Methyltransf_4. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53335. SSF53335. 1 hit.
    TIGRFAMsi TIGR00091. TIGR00091. 1 hit.
    PROSITEi PS51625. SAM_MT_TRMB. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. "Two-dimensional map of the proteome of Haemophilus influenzae."
      Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M.
      Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiTRMB_HAEIN
    AccessioniPrimary (citable) accession number: P44648
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 97 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3