Reviewed, UniProtKB/Swiss-Prot P44638 (LGUL_HAEIN)

Names and origin

Protein names

Recommended name:
    Lactoylglutathione lyase
    EC=4.4.1.5
Alternative name(s):
    Methylglyoxalase
    Aldoketomutase
    Glyoxalase I
      Short name=Glx I
    Ketone-aldehyde mutase
    S-D-lactoylglutathione methylglyoxal lyase

Gene names

Name:	gloA
Ordered Locus Names:	HI0323

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	135 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Catalyzes the conversion of hemimercaptal, formed from methylglyoxal and glutathione, to S-lactoylglutathione By similarity.
Catalytic activity	(R)-S-lactoylglutathione = glutathione + methylglyoxal.
Cofactor	Binds 1 nickel ion per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 1/2.
Sequence similarities	Belongs to the glyoxalase I family.

Ontologies

Keywords
Ligand	Metal-binding Nickel
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	lactoylglutathione lyase activity Inferred from electronic annotation. Source: EC nickel ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 135	135	Lactoylglutathione lyase		PRO_0000168093
Sites
	Metal binding	5	1	Nickel By similarity
	Metal binding	56	1	Nickel By similarity
	Metal binding	74	1	Nickel By similarity
	Metal binding	122	1	Nickel By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44638-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B6DF5FFCD9669E80	FASTA	135	14,893
	10 20 30 40 50 60 MQILHTMLRV GDLDRSIKFY QDVLGMRLLR TSENPEYKYT LAFLGYEDGE SAAEIELTYN 70 80 90 100 110 120 WGVDKYEHGT AYGHIAIGVD DIYATCEAVR ASGGNVTREA GPVKGGSTVI AFVEDPDGYK 130 IEFIENKSTK SGLGN

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Reference map of the low molecular mass proteins of Haemophilus influenzae." Fountoulakis M., Juranville J.-F., Roeder D., Evers S., Berndt P., Langen H. Electrophoresis 19:1819-1827(1998) [PubMed: 9719565] [Abstract] Cited for: IDENTIFICATION BY MASS SPECTROMETRY.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21986.1.
PIR	I64147.
RefSeq	NP_438488.1.
3D structure databases
SMR	P44638. Positions 1-125.
ModBase	Search...
Genome annotation databases
GeneID	949438.
GenomeReviews	Gene locus HI0323 in contig L42023_GR.
KEGG	hin:HI0323.
TIGR	HI0323.
Phylogenomic databases
HOGENOM	HBG704761.
OMA	PGPMKHG.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0323-MONOMER.
BRENDA	4.4.1.5. 109.
Family and domain databases
InterPro	IPR004360. Glyas_bleo-R_dOase. IPR004361. Glyoxalase_1. IPR018146. Glyoxalase_1_CS. [Graphical view]
Pfam	PF00903. Glyoxalase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00068. glyox_I. 1 hit.
PROSITE	PS00934. GLYOXALASE_I_1. 1 hit. PS00935. GLYOXALASE_I_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LGUL_HAEIN

Accession

Primary (citable) accession number: P44638

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	February 9, 2010
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)