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Protein

Ribosomal RNA small subunit methyltransferase E

Gene

rsmE

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Specifically methylates the N3 position of the uracil ring of uridine 1498 (m3U1498) in 16S rRNA. Acts on the fully assembled 30S ribosomal subunit (By similarity).By similarity

Catalytic activityi

S-adenosyl-L-methionine + uracil(1498) in 16S rRNA = S-adenosyl-L-homocysteine + N(3)-methyluracil(1498) in 16S rRNA.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

rRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Names & Taxonomyi

Protein namesi
Recommended name:
Ribosomal RNA small subunit methyltransferase E (EC:2.1.1.193)
Alternative name(s):
16S rRNA m3U1498 methyltransferase
Gene namesi
Name:rsmE
Ordered Locus Names:HI_0303
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 245245Ribosomal RNA small subunit methyltransferase EPRO_0000176202Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0303.

Structurei

Secondary structure

1
245
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi5 – 73Combined sources
Beta strandi16 – 194Combined sources
Helixi22 – 298Combined sources
Beta strandi39 – 435Combined sources
Beta strandi45 – 5713Combined sources
Beta strandi62 – 665Combined sources
Beta strandi80 – 856Combined sources
Beta strandi88 – 903Combined sources
Helixi93 – 1019Combined sources
Beta strandi106 – 1116Combined sources
Beta strandi115 – 1173Combined sources
Helixi121 – 14222Combined sources
Helixi156 – 1605Combined sources
Beta strandi167 – 1715Combined sources
Helixi179 – 1813Combined sources
Beta strandi190 – 1945Combined sources
Helixi202 – 2109Combined sources
Beta strandi214 – 2174Combined sources
Helixi225 – 23915Combined sources
Helixi242 – 2443Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NXZX-ray2.00A/B2-245[»]
1VHYX-ray1.90A/B2-245[»]
ProteinModelPortaliP44627.
SMRiP44627. Positions 2-245.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44627.

Family & Domainsi

Sequence similaritiesi

Belongs to the RNA methyltransferase RsmE family.Curated

Phylogenomic databases

eggNOGiENOG4105QF0. Bacteria.
COG1385. LUCA.
KOiK09761.
OMAiERMEFTI.
OrthoDBiEOG6FJNHQ.
PhylomeDBiP44627.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR015947. PUA-like_domain.
IPR006700. rRNA_ssu_MeTrfase-E.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04452. Methyltrans_RNA. 1 hit.
[Graphical view]
PIRSFiPIRSF015601. MTase_slr0722. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00046. TIGR00046. 1 hit.

Sequencei

Sequence statusi: Complete.

P44627-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIPRIYHPI SLENQTQCYL SEDAANHVAR VLRMTEGEQL ELFDGSNHIY
60 70 80 90 100
PAKIIESNKK SVKVEILGRE LADKESHLKI HLGQVISRGE RMEFTIQKSV
110 120 130 140 150
ELGVNVITPL WSERCGVKLD AERMDKKIQQ WQKIAIAACE QCGRNIVPEI
160 170 180 190 200
RPLMKLQDWC AENDGALKLN LHPRAHYSIK TLPTIPAGGV RLLIGSEGGL
210 220 230 240
SAQEIAQTEQ QGFTEILLGK RVLRTETASL AAISALQICF GDLGE
Length:245
Mass (Da):27,373
Last modified:November 1, 1995 - v1
Checksum:i1F6BEA83A791328C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21968.1.
PIRiD64147.
RefSeqiNP_438470.1. NC_000907.1.
WP_005694361.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21968; AAC21968; HI_0303.
GeneIDi950245.
KEGGihin:HI0303.
PATRICi20189147. VBIHaeInf48452_0320.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21968.1.
PIRiD64147.
RefSeqiNP_438470.1. NC_000907.1.
WP_005694361.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1NXZX-ray2.00A/B2-245[»]
1VHYX-ray1.90A/B2-245[»]
ProteinModelPortaliP44627.
SMRiP44627. Positions 2-245.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21968; AAC21968; HI_0303.
GeneIDi950245.
KEGGihin:HI0303.
PATRICi20189147. VBIHaeInf48452_0320.

Phylogenomic databases

eggNOGiENOG4105QF0. Bacteria.
COG1385. LUCA.
KOiK09761.
OMAiERMEFTI.
OrthoDBiEOG6FJNHQ.
PhylomeDBiP44627.

Miscellaneous databases

EvolutionaryTraceiP44627.

Family and domain databases

Gene3Di3.40.1280.10. 1 hit.
InterProiIPR029028. Alpha/beta_knot_MTases.
IPR015947. PUA-like_domain.
IPR006700. rRNA_ssu_MeTrfase-E.
IPR029026. tRNA_m1G_MTases_N.
[Graphical view]
PfamiPF04452. Methyltrans_RNA. 1 hit.
[Graphical view]
PIRSFiPIRSF015601. MTase_slr0722. 1 hit.
SUPFAMiSSF75217. SSF75217. 1 hit.
SSF88697. SSF88697. 1 hit.
TIGRFAMsiTIGR00046. TIGR00046. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
  2. "Functional assignment based on structural analysis: crystal structure of the yggJ protein (HI0303) of Haemophilus influenzae reveals an RNA methyltransferase with a deep trefoil knot."
    Forouhar F., Shen J., Xiao R., Acton T.B., Montelione G.T., Tong L.
    Proteins 53:329-332(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.0 ANGSTROMS) OF 2-245, IDENTIFICATION AS PUTATIVE RNA METHYLTRANSFERASE.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 2-245.

Entry informationi

Entry nameiRSME_HAEIN
AccessioniPrimary (citable) accession number: P44627
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: June 8, 2016
This is version 108 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.