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P44624

- AMPD_HAEIN

UniProt

P44624 - AMPD_HAEIN

Protein

1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD

Gene

ampD

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 87 (01 Oct 2014)
      Sequence version 1 (01 Nov 1995)
      Previous versions | rss
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    Functioni

    Involved in both cell wall peptidoglycans recycling and beta-lactamase induction. Specifically cleaves the amide bond between the lactyl group of N-acetylmuramic acid and the alpha-amino group of the L-alanine in degradation products containing an anhydro N-acetylmuramyl moiety By similarity.By similarity

    Catalytic activityi

    Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.

    Cofactori

    Zinc; required for amidase activity.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi38 – 381Zinc; catalyticBy similarity
    Active sitei120 – 1201Proton acceptorBy similarity
    Metal bindingi159 – 1591Zinc; catalyticBy similarity
    Sitei167 – 1671Transition state stabilizerBy similarity
    Metal bindingi169 – 1691Zinc; catalyticBy similarity

    GO - Molecular functioni

    1. metal ion binding Source: UniProtKB-KW
    2. N-acetylmuramoyl-L-alanine amidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. peptidoglycan catabolic process Source: InterPro

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Cell wall biogenesis/degradation

    Keywords - Ligandi

    Metal-binding, Zinc

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD (EC:3.5.1.28)
    Alternative name(s):
    N-acetylmuramoyl-L-alanine amidase
    Gene namesi
    Name:ampD
    Ordered Locus Names:HI_0300
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 1841841,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpDPRO_0000164414Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0300.

    Structurei

    3D structure databases

    ProteinModelPortaliP44624.
    SMRiP44624. Positions 6-183.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG3023.
    KOiK03806.
    OMAiCEDRAWH.
    OrthoDBiEOG60GRR5.
    PhylomeDBiP44624.

    Family and domain databases

    Gene3Di3.40.80.10. 1 hit.
    InterProiIPR002502. Amidase_domain.
    [Graphical view]
    PfamiPF01510. Amidase_2. 1 hit.
    [Graphical view]
    SMARTiSM00644. Ami_2. 1 hit.
    [Graphical view]
    SUPFAMiSSF55846. SSF55846. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P44624-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRKIKDIEKG LLTDCRQIQS PHFDKRPNPQ DISLLVIHYI SLPPEQFGGG    50
    YVDDFFQGKL DPKIHPYFAE IYQMRVSAHC LIERNGRITQ YVNFNDRAWH 100
    AGVSNFQGRE KCNDFAIGIE LEGSNEQPFT DAQYFSLQEL TNVIMKSYPK 150
    ITKDRIVGHC DISPKRKIDP GQYFDWERYL SSVK 184
    Length:184
    Mass (Da):21,448
    Last modified:November 1, 1995 - v1
    Checksum:iB7A035527E328401
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21965.1.
    PIRiF64060.
    RefSeqiNP_438467.1. NC_000907.1.
    WP_005694366.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC21965; AAC21965; HI_0300.
    GeneIDi949420.
    KEGGihin:HI0300.
    PATRICi20189141. VBIHaeInf48452_0317.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21965.1 .
    PIRi F64060.
    RefSeqi NP_438467.1. NC_000907.1.
    WP_005694366.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44624.
    SMRi P44624. Positions 6-183.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0300.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC21965 ; AAC21965 ; HI_0300 .
    GeneIDi 949420.
    KEGGi hin:HI0300.
    PATRICi 20189141. VBIHaeInf48452_0317.

    Phylogenomic databases

    eggNOGi COG3023.
    KOi K03806.
    OMAi CEDRAWH.
    OrthoDBi EOG60GRR5.
    PhylomeDBi P44624.

    Family and domain databases

    Gene3Di 3.40.80.10. 1 hit.
    InterProi IPR002502. Amidase_domain.
    [Graphical view ]
    Pfami PF01510. Amidase_2. 1 hit.
    [Graphical view ]
    SMARTi SM00644. Ami_2. 1 hit.
    [Graphical view ]
    SUPFAMi SSF55846. SSF55846. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiAMPD_HAEIN
    AccessioniPrimary (citable) accession number: P44624
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: November 1, 1995
    Last modified: October 1, 2014
    This is version 87 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3