Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphatidylglycerophosphatase B

Gene

pgpB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the dephosphorylation of diacylglycerol diphosphate (DGPP) to phosphatidate (PA) and the subsequent dephosphorylation of PA to diacylglycerol (DAG). Also has undecaprenyl pyrophosphate phosphatase activity, required for the biosynthesis of the lipid carrier undecaprenyl phosphate. Can also use lysophosphatidic acid (LPA) and phosphatidylglycerophosphate as substrates. The pattern of activities varies according to subcellular location, PGP phosphatase activity is higher in the cytoplasmic membrane, whereas PA and LPA phosphatase activities are higher in the outer membrane. Activity is independent of a divalent cation ion and insensitive to inhibition by N-ethylmaleimide (By similarity).By similarity

Catalytic activityi

Phosphatidylglycerophosphate + H2O = phosphatidylglycerol + phosphate.
1,2-diacyl-sn-glycerol 3-diphosphate + H2O = 1,2-diacyl-sn-glycerol 3-phosphate + phosphate.
A 1,2-diacylglycerol 3-phosphate + H2O = a 1,2-diacyl-sn-glycerol + phosphate.
Ditrans,octacis-undecaprenyl diphosphate + H2O = ditrans,octacis-undecaprenyl phosphate + phosphate.

Pathwayi: phosphatidylglycerol biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. CDP-diacylglycerol--glycerol-3-phosphate 3-phosphatidyltransferase (pgsA)
  2. Phosphatidylglycerophosphatase B (pgpB), Phosphatidylglycerophosphatase A (pgpA)
This subpathway is part of the pathway phosphatidylglycerol biosynthesis, which is itself part of Phospholipid metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes phosphatidylglycerol from CDP-diacylglycerol, the pathway phosphatidylglycerol biosynthesis and in Phospholipid metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei160Proton donor; for a subset of substratesBy similarity1
Active sitei209NucleophileBy similarity1
Sitei213Stabilizes the active site histidine for nucleophilic attackBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase
Biological processLipid degradation, Lipid metabolism, Phospholipid degradation, Phospholipid metabolism

Enzyme and pathway databases

UniPathwayiUPA00084; UER00504.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphatidylglycerophosphatase B (EC:3.1.3.27)
Alternative name(s):
Diacylglycerol pyrophosphate phosphatase (EC:3.1.3.81)
Short name:
DGPP phosphatase
Phosphatidate phosphatase (EC:3.1.3.4)
Undecaprenyl pyrophosphate phosphatase (EC:3.6.1.27)
Undecaprenyl-diphosphatase
Gene namesi
Name:pgpB
Ordered Locus Names:HI_0211
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei1 – 21HelicalBy similarityAdd BLAST21
Topological domaini22 – 52PeriplasmicBy similarityAdd BLAST31
Transmembranei53 – 62HelicalBy similarity10
Topological domaini63 – 67CytoplasmicBy similarity5
Transmembranei68 – 91HelicalBy similarityAdd BLAST24
Topological domaini92 – 158PeriplasmicBy similarityAdd BLAST67
Transmembranei159 – 173HelicalBy similarityAdd BLAST15
Topological domaini174 – 184CytoplasmicBy similarityAdd BLAST11
Transmembranei185 – 204HelicalBy similarityAdd BLAST20
Topological domaini205 – 210PeriplasmicBy similarity6
Transmembranei211 – 235HelicalBy similarityAdd BLAST25
Topological domaini236 – 241CytoplasmicBy similarity6

GO - Cellular componenti

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Cell outer membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000583631 – 241Phosphatidylglycerophosphatase BAdd BLAST241

Interactioni

Protein-protein interaction databases

STRINGi71421.HI0211.

Structurei

3D structure databases

ProteinModelPortaliP44570.
SMRiP44570.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni94 – 102Phosphatase sequence motif ICurated9
Regioni157 – 160Phosphatase sequence motif IICurated4
Regioni202 – 213Phosphatase sequence motif IIICuratedAdd BLAST12

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EYK. Bacteria.
COG0671. LUCA.
KOiK01096.
OMAiAWFLWCL.
PhylomeDBiP44570.

Family and domain databases

Gene3Di1.20.144.10. 1 hit.
InterProiView protein in InterPro
IPR000326. P_Acid_Pase_2/haloperoxidase.
PfamiView protein in Pfam
PF01569. PAP2. 1 hit.
SMARTiView protein in SMART
SM00014. acidPPc. 1 hit.
SUPFAMiSSF48317. SSF48317. 2 hits.

Sequencei

Sequence statusi: Complete.

P44570-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MFKRLSLYTL LLCLVPFFIW GISYQWHGNS QLTQADYWLY LLTETGSVPY
60 70 80 90 100
ALITCVLFTL LFAFLFKNPK QWILGVIVMG ISVIATQAAK TGAKALFEEP
110 120 130 140 150
RPFTVYLAEQ THSTPENFYK NDRTLRAEIA KNFYSMDAIT PAWLVHHYEN
160 170 180 190 200
ETGYSFPSGH TIFAATWLML AVGFTQLLGN RSFKAKLLVV GIAVWGLLML
210 220 230 240
ISRVRLGMHY PIDLLVATLL AWLINSIIFA FLKKKAIFVM K
Length:241
Mass (Da):27,491
Last modified:November 1, 1995 - v1
Checksum:i86896C8B066D2A41
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21879.1.
PIRiI64054.
RefSeqiNP_438379.1. NC_000907.1.
WP_005694086.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21879; AAC21879; HI_0211.
GeneIDi951120.
KEGGihin:HI0211.
PATRICifig|71421.8.peg.215.

Similar proteinsi

Entry informationi

Entry nameiPGPB_HAEIN
AccessioniPrimary (citable) accession number: P44570
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 27, 2017
This is version 102 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families