Reviewed, UniProtKB/Swiss-Prot P44569 (5NTD_HAEIN)

Names and origin

Protein names

Recommended name:
    Probable 5'-nucleotidase
    EC=3.1.3.5

Gene names

Ordered Locus Names:

HI0206

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	603 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.
Cofactor	Divalent cations By similarity.
Sequence similarities	Belongs to the 5'-nucleotidase family.

Ontologies

Keywords
Domain	Signal
Ligand	Metal-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	nucleotide catabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	5'-nucleotidase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotide binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Ref.2
	Chain	26 – 603	578	Probable 5'-nucleotidase		PRO_0000000026
Regions
	Region	540 – 546	7	Substrate binding By similarity
Sites
	Metal binding	44	1	Divalent metal cation 1 By similarity
	Metal binding	46	1	Divalent metal cation 1 By similarity
	Metal binding	94	1	Divalent metal cation 1 By similarity
	Metal binding	94	1	Divalent metal cation 2 By similarity
	Metal binding	126	1	Divalent metal cation 2 By similarity
	Metal binding	227	1	Divalent metal cation 2 By similarity
	Binding site	456	1	Substrate By similarity
	Site	127	1	Transition state stabilizer By similarity
	Site	130	1	Transition state stabilizer By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44569-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: B641C32A5A2B6AEA	FASTA	603	66,245
	10 20 30 40 50 60 MLLSKKSASF ALSAFAMLFT SVALAKEAPQ AHKAVELSIL HINDHHSYLE PHETRINLNG 70 80 90 100 110 120 QQTKVDIGGF SAVNAKLNKL RKKYKNPLVL HAGDAITGTL YFTLFGGSAD AAVMNAGNFH 130 140 150 160 170 180 YFTLGNHEFD AGNEGLLKLL EPLKIPVLSA NVIPDKNSIL YNKWKPYDIF TVDGEKIAII 190 200 210 220 230 240 GLDTVNKTVN SSSPGKDVKF YDEIATAQIM ANALKQQGIN KIILLSHAGS EKNIEIAQKV 250 260 270 280 290 300 NDIDVIVTGD SHYLYGNDEL RSLKLPVIYE YPLEFKNPNG DPVFVMEGWA YSAVVGDLGV 310 320 330 340 350 360 KFSPEGIASI TRKIPHVLMS SHKLQVKNAE GKWTELTGDE RKKALDTLKS MKSISLDDHD 370 380 390 400 410 420 AKTDMLISKY KSEKDRLAQE IVGVITGSAM PGGSANRIPN KAGSNPEGSI ATRFIAETMY 430 440 450 460 470 480 NELKTVDLTI QNAGGVRADI LPGNVTFNDA YTFLPFGNTL YTYKMEGSLV KQVLEDAMQF 490 500 510 520 530 540 ALVDGSTGAF PYGAGIRYEA NETPNAEGKR LVSVEVLNKQ TQQWEPIDDN KRYLVGTNAY 550 560 570 580 590 600 VAGGKDGYKT FGKLFNDPKY EGVDTYLPDA ESFIKFMKKH PHFEAYTSSN VKFNASTDAL PKK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
[2]	"Two-dimensional map of the proteome of Haemophilus influenzae." Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M. Electrophoresis 21:411-429(2000) [PubMed: 10675023] [Abstract] Cited for: PROTEIN SEQUENCE OF 26-38.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21874.1.
PIR	E64054.
RefSeq	NP_438375.1.
3D structure databases
HSSP	HSSP built from PDB template 2USH based on UniProtKB P07024.
ModBase	Search...
Genome annotation databases
GeneID	951116.
GenomeReviews	Gene locus HI0206 in contig L42023_GR.
KEGG	hin:HI0206.
TIGR	HI0206.
Phylogenomic databases
HOGENOM	P44569.
OMA	FLHAGDC.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0206-MON. MetaCyc:MON-8341.
BRENDA	3.1.3.5. 109.
Family and domain databases
InterPro	IPR008334. 5'-Nucleotdase_C. IPR006146. 5'-Nucleotdase_CS. IPR006179. 5_nucleotidase/apyrase. IPR004843. M-pesterase. IPR006420. NadN. [Graphical view]
Gene3D	G3DSA:3.90.780.10. 5'-Nucleotdase_C. 1 hit.
PANTHER	PTHR11575. 5_nucleotidase. 1 hit.
Pfam	PF02872. 5_nucleotid_C. 1 hit. PF00149. Metallophos. 1 hit. [Graphical view]
PRINTS	PR01607. APYRASEFAMLY.
TIGRFAMs	TIGR01530. nadN. 1 hit.
PROSITE	PS00785. 5_NUCLEOTIDASE_1. 1 hit. PS00786. 5_NUCLEOTIDASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

5NTD_HAEIN

Accession

Primary (citable) accession number: P44569

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)