P44557 (FRMA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
S-(hydroxymethyl)glutathione dehydrogenase
EC=1.1.1.284

Alternative name(s):
Alcohol dehydrogenase class-3
EC=1.1.1.1
Alcohol dehydrogenase class-III
Glutathione-dependent formaldehyde dehydrogenase
Short name=FALDH
Short name=FDH
Short name=GSH-FDH
EC=1.1.1.-

Gene names

Name:	frmA
Synonyms:	adhC
Ordered Locus Names:	HI_0185

Organism

Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]

Taxonomic identifier

71421 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus › Haemophilus influenzae

Protein attributes

Sequence length	378 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	S-(hydroxymethyl)glutathione + NAD(P)+ = S-formylglutathione + NAD(P)H. An alcohol + NAD+ = an aldehyde or ketone + NADH.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Subcellular location	Cytoplasm Potential.
Sequence similarities	Belongs to the zinc-containing alcohol dehydrogenase family. Class-III subfamily.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	Metal-binding NAD Zinc
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ethanol oxidation Inferred from electronic annotation. Source: InterPro
Cellular_component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	S-(hydroxymethyl)glutathione dehydrogenase activity Inferred from electronic annotation. Source: EC alcohol dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC nucleotide binding Inferred from electronic annotation. Source: InterPro zinc ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 378	378	S-(hydroxymethyl)glutathione dehydrogenase		PRO_0000160776
Sites
	Metal binding	49	1	Zinc 1; catalytic By similarity
	Metal binding	71	1	Zinc 1; catalytic By similarity
	Metal binding	101	1	Zinc 2 By similarity
	Metal binding	104	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	115	1	Zinc 2 By similarity
	Metal binding	178	1	Zinc 1; catalytic By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44557 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: A68677243681165E	FASTA	378	40,651
	10 20 30 40 50 60 MEIKQINSTI KSRAAVAFAP NQPLQIVEID VEMPRKGEVL IRNTHTGVCH TDAFTLSGSD 70 80 90 100 110 120 PEGVFPVVLG HEGAGVVVAV GEGVLSVKPG DHVIPLYTAE CGECEFCRSG KTNLCVSVRD 130 140 150 160 170 180 TQGKGLMPDC TTRFSYQGQP IYHYMGCSTF SEYSVVAEVS LAKINPEANH EQVCLLGCGV 190 200 210 220 230 240 TTGIGAVHNT AKVQEGDSVA VFGLGAIGLA VVQGARQAKA GRIIAIDTNP AKFELAKQFG 250 260 270 280 290 300 ATDCLNPNDY DKPIKDVLLD INKWGIDHTF ECIGNVNVMR QALESAHRGW GQSIIIGVAG 310 320 330 340 350 360 AGQEISTRPF QLVTGRVWKG SAFGGVKGRS ELPQMVEDSM KGDIQLEPFV THTMPLDKIN 370 EAFELMHEGK SIRTVIHY

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

+

Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21854.1.
PIR	H64052.
RefSeq	NP_438353.1. NC_000907.1.
3D structure databases
ProteinModelPortal	P44557.
SMR	P44557. Positions 7-377.
ModBase	Search...
Protein-protein interaction databases
STRING	71421.HI0185.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC21854; AAC21854; HI_0185.
GeneID	951091.
KEGG	hin:HI0185.
PATRIC	20188865. VBIHaeInf48452_0189.
Phylogenomic databases
eggNOG	COG1062.
KO	K00121.
OMA	VDHTFEC.
ProtClustDB	CLSK2393140.
Family and domain databases
Gene3D	3.40.50.720. 1 hit.
InterPro	IPR014183. ADH_3. IPR013149. ADH_C. IPR013154. ADH_GroES-like. IPR002085. ADH_SF_Zn-type. IPR002328. ADH_Zn_CS. IPR011032. GroES-like. IPR016040. NAD(P)-bd_dom. [Graphical view]
PANTHER	PTHR11695. PTHR11695. 1 hit.
Pfam	PF08240. ADH_N. 1 hit. PF00107. ADH_zinc_N. 1 hit. [Graphical view]
SUPFAM	SSF50129. GroES_like. 2 hits.
TIGRFAMs	TIGR02818. adh_III_F_hyde. 1 hit.
PROSITE	PS00059. ADH_ZINC. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

FRMA_HAEIN

Accession

Primary (citable) accession number: P44557

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	May 1, 2013
This is version 100 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program