Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

N-acetylneuraminate lyase

Gene

nanA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei136 – 1361Involved in proton transfer during cleavage
Active sitei164 – 1641Schiff-base intermediate with substrate

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NAL
Short name:
Neu5Ac lyase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:nanA
Ordered Locus Names:HI_0142
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 293293N-acetylneuraminate lyasePRO_0000103214Add
BLAST

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi71421.HI0142.

Structurei

Secondary structure

1
293
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi6 – 105Combined sources
Beta strandi19 – 213Combined sources
Helixi23 – 3513Combined sources
Beta strandi40 – 467Combined sources
Helixi47 – 493Combined sources
Helixi51 – 533Combined sources
Helixi56 – 7015Combined sources
Beta strandi73 – 797Combined sources
Helixi85 – 9814Combined sources
Beta strandi101 – 1066Combined sources
Helixi115 – 12915Combined sources
Beta strandi133 – 1375Combined sources
Helixi139 – 1424Combined sources
Helixi148 – 1558Combined sources
Beta strandi160 – 1656Combined sources
Helixi170 – 17910Combined sources
Beta strandi183 – 1875Combined sources
Helixi190 – 1923Combined sources
Helixi193 – 1986Combined sources
Beta strandi202 – 2076Combined sources
Helixi209 – 22416Combined sources
Helixi228 – 24821Combined sources
Helixi250 – 26011Combined sources
Helixi278 – 29114Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F5ZX-ray1.88A/B/C/D1-293[»]
1F6KX-ray1.60A/C1-293[»]
1F6PX-ray2.25A/B/C/D1-293[»]
1F73X-ray1.95A/B/C/D1-293[»]
1F74X-ray1.60A/C1-293[»]
1F7BX-ray1.80A/C1-293[»]
ProteinModelPortaliP44539.
SMRiP44539. Positions 1-293.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44539.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni47 – 482Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CDP. Bacteria.
COG0329. LUCA.
KOiK01639.
OMAiYWNAISA.
PhylomeDBiP44539.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01237. N_acetylneuram_lyase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR005264. NanA.
IPR020625. Schiff_base-form_aldolases_AS.
IPR020624. Schiff_base-form_aldolases_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00683. nanA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDLKGIFSA LLVSFNEDGT INEKGLRQII RHNIDKMKVD GLYVGGSTGE
60 70 80 90 100
NFMLSTEEKK EIFRIAKDEA KDQIALIAQV GSVNLKEAVE LGKYATELGY
110 120 130 140 150
DCLSAVTPFY YKFSFPEIKH YYDTIIAETG NNMIVYSIPF LTGVNMGIEQ
160 170 180 190 200
FGELYKNPKV LGVKFTAGDF YLLERLKKAY PNHLIWAGFD EMMLPAASLG
210 220 230 240 250
VDGAIGSTFN VNGVRARQIF ELTKAGKLAE ALEIQHVTND LIEGILANGL
260 270 280 290
YLTIKELLKL EGVDAGYCRE PMTSKATEEQ LAKAKDLKAK FLS
Length:293
Mass (Da):32,565
Last modified:November 1, 1995 - v1
Checksum:iACA755D0A5D93D33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21814.1.
PIRiG64050.
RefSeqiNP_438311.1. NC_000907.1.
WP_005694428.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21814; AAC21814; HI_0142.
GeneIDi951045.
KEGGihin:HI0142.
PATRICi20188775. VBIHaeInf48452_0144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21814.1.
PIRiG64050.
RefSeqiNP_438311.1. NC_000907.1.
WP_005694428.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1F5ZX-ray1.88A/B/C/D1-293[»]
1F6KX-ray1.60A/C1-293[»]
1F6PX-ray2.25A/B/C/D1-293[»]
1F73X-ray1.95A/B/C/D1-293[»]
1F74X-ray1.60A/C1-293[»]
1F7BX-ray1.80A/C1-293[»]
ProteinModelPortaliP44539.
SMRiP44539. Positions 1-293.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21814; AAC21814; HI_0142.
GeneIDi951045.
KEGGihin:HI0142.
PATRICi20188775. VBIHaeInf48452_0144.

Phylogenomic databases

eggNOGiENOG4105CDP. Bacteria.
COG0329. LUCA.
KOiK01639.
OMAiYWNAISA.
PhylomeDBiP44539.

Miscellaneous databases

EvolutionaryTraceiP44539.
PROiP44539.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01237. N_acetylneuram_lyase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR005264. NanA.
IPR020625. Schiff_base-form_aldolases_AS.
IPR020624. Schiff_base-form_aldolases_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00683. nanA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANA_HAEIN
AccessioniPrimary (citable) accession number: P44539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: September 7, 2016
This is version 113 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.