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Protein

N-acetylneuraminate lyase

Gene

nanA

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the reversible aldol cleavage of N-acetylneuraminic acid (sialic acid; Neu5Ac) to form pyruvate and N-acetylmannosamine (ManNAc) via a Schiff base intermediate.

Catalytic activityi

N-acetylneuraminate = N-acetyl-D-mannosamine + pyruvate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei136Involved in proton transfer during cleavage1
Active sitei164Schiff-base intermediate with substrate1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Carbohydrate metabolism

Keywords - Ligandi

Schiff base

Names & Taxonomyi

Protein namesi
Recommended name:
N-acetylneuraminate lyase (EC:4.1.3.3)
Short name:
NAL
Short name:
Neu5Ac lyase
Alternative name(s):
N-acetylneuraminate pyruvate-lyase
N-acetylneuraminic acid aldolase
Sialate lyase
Sialic acid aldolase
Sialic acid lyase
Gene namesi
Name:nanA
Ordered Locus Names:HI_0142
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001032141 – 293N-acetylneuraminate lyaseAdd BLAST293

Interactioni

Subunit structurei

Homotetramer.

Protein-protein interaction databases

STRINGi71421.HI0142.

Structurei

Secondary structure

1293
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi6 – 10Combined sources5
Beta strandi19 – 21Combined sources3
Helixi23 – 35Combined sources13
Beta strandi40 – 46Combined sources7
Helixi47 – 49Combined sources3
Helixi51 – 53Combined sources3
Helixi56 – 70Combined sources15
Beta strandi73 – 79Combined sources7
Helixi85 – 98Combined sources14
Beta strandi101 – 106Combined sources6
Helixi115 – 129Combined sources15
Beta strandi133 – 137Combined sources5
Helixi139 – 142Combined sources4
Helixi148 – 155Combined sources8
Beta strandi160 – 165Combined sources6
Helixi170 – 179Combined sources10
Beta strandi183 – 187Combined sources5
Helixi190 – 192Combined sources3
Helixi193 – 198Combined sources6
Beta strandi202 – 207Combined sources6
Helixi209 – 224Combined sources16
Helixi228 – 248Combined sources21
Helixi250 – 260Combined sources11
Helixi278 – 291Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F5ZX-ray1.88A/B/C/D1-293[»]
1F6KX-ray1.60A/C1-293[»]
1F6PX-ray2.25A/B/C/D1-293[»]
1F73X-ray1.95A/B/C/D1-293[»]
1F74X-ray1.60A/C1-293[»]
1F7BX-ray1.80A/C1-293[»]
ProteinModelPortaliP44539.
SMRiP44539.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP44539.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni47 – 48Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the DapA family. NanA subfamily.Curated

Phylogenomic databases

eggNOGiENOG4105CDP. Bacteria.
COG0329. LUCA.
KOiK01639.
OMAiYWNAISA.
PhylomeDBiP44539.

Family and domain databases

CDDicd00954. NAL. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01237. N_acetylneuram_lyase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR005264. NanA.
IPR020625. Schiff_base-form_aldolases_AS.
IPR020624. Schiff_base-form_aldolases_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00683. nanA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44539-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRDLKGIFSA LLVSFNEDGT INEKGLRQII RHNIDKMKVD GLYVGGSTGE
60 70 80 90 100
NFMLSTEEKK EIFRIAKDEA KDQIALIAQV GSVNLKEAVE LGKYATELGY
110 120 130 140 150
DCLSAVTPFY YKFSFPEIKH YYDTIIAETG NNMIVYSIPF LTGVNMGIEQ
160 170 180 190 200
FGELYKNPKV LGVKFTAGDF YLLERLKKAY PNHLIWAGFD EMMLPAASLG
210 220 230 240 250
VDGAIGSTFN VNGVRARQIF ELTKAGKLAE ALEIQHVTND LIEGILANGL
260 270 280 290
YLTIKELLKL EGVDAGYCRE PMTSKATEEQ LAKAKDLKAK FLS
Length:293
Mass (Da):32,565
Last modified:November 1, 1995 - v1
Checksum:iACA755D0A5D93D33
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21814.1.
PIRiG64050.
RefSeqiNP_438311.1. NC_000907.1.
WP_005694428.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21814; AAC21814; HI_0142.
GeneIDi951045.
KEGGihin:HI0142.
PATRICi20188775. VBIHaeInf48452_0144.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21814.1.
PIRiG64050.
RefSeqiNP_438311.1. NC_000907.1.
WP_005694428.1. NC_000907.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1F5ZX-ray1.88A/B/C/D1-293[»]
1F6KX-ray1.60A/C1-293[»]
1F6PX-ray2.25A/B/C/D1-293[»]
1F73X-ray1.95A/B/C/D1-293[»]
1F74X-ray1.60A/C1-293[»]
1F7BX-ray1.80A/C1-293[»]
ProteinModelPortaliP44539.
SMRiP44539.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0142.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21814; AAC21814; HI_0142.
GeneIDi951045.
KEGGihin:HI0142.
PATRICi20188775. VBIHaeInf48452_0144.

Phylogenomic databases

eggNOGiENOG4105CDP. Bacteria.
COG0329. LUCA.
KOiK01639.
OMAiYWNAISA.
PhylomeDBiP44539.

Miscellaneous databases

EvolutionaryTraceiP44539.
PROiP44539.

Family and domain databases

CDDicd00954. NAL. 1 hit.
Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01237. N_acetylneuram_lyase. 1 hit.
InterProiIPR013785. Aldolase_TIM.
IPR002220. DapA-like.
IPR005264. NanA.
IPR020625. Schiff_base-form_aldolases_AS.
IPR020624. Schiff_base-form_aldolases_CS.
[Graphical view]
PANTHERiPTHR12128. PTHR12128. 1 hit.
PfamiPF00701. DHDPS. 1 hit.
[Graphical view]
PIRSFiPIRSF001365. DHDPS. 1 hit.
PRINTSiPR00146. DHPICSNTHASE.
SMARTiSM01130. DHDPS. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00683. nanA. 1 hit.
PROSITEiPS00665. DHDPS_1. 1 hit.
PS00666. DHDPS_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiNANA_HAEIN
AccessioniPrimary (citable) accession number: P44539
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 2, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.