Reviewed, UniProtKB/Swiss-Prot P44527 (METC_HAEIN)

Names and origin

Protein names

Recommended name:
    Cystathionine beta-lyase
      Short name=CBL
    EC=4.4.1.8
Alternative name(s):
    Beta-cystathionase
    Cysteine lyase

Gene names

Name:	metC
Ordered Locus Names:	HI0122

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-cystathionine + H2O = L-homocysteine + NH3 + pyruvate.
Cofactor	Pyridoxal phosphate By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homocysteine from L-cystathionine: step 1/1.
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the trans-sulfuration enzymes family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Cellular component	Cytoplasm
Ligand	Pyridoxal phosphate
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cystathionine beta-lyase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 396	396	Cystathionine beta-lyase		PRO_0000114770
Amino acid modifications
	Modified residue	211	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44527-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 5ABFD4A3C4F991EE	FASTA	396	44,345
	10 20 30 40 50 60 MQTKYDLSTM FIHSGRQKRF SQGSVNPVLQ RASSLLFDSI EDKKHATQRR AKGELFYGRR 70 80 90 100 110 120 GTLTHFALQD LMCEMEGGAG CYLYPCGTAA VTNSILSFVK TGDHVLMSGA AYEPTQYFCN 130 140 150 160 170 180 IVLKKMQIDI TYYDPLIGED IATLIQPNTK VLFLEAPSSI TMEIPDIPTI VKAARKVNPN 190 200 210 220 230 240 IVIMIDNTWS AGVLFKALEH DIDISIQAGT KYLVGHSDIM IGTAVANART WDQLREHSYL 250 260 270 280 290 300 MGQMVDADSA YTTARGIRTL GVRLKQHQES SIKVAKWLSE QPEVKTVYHP ALPSCPGHEF 310 320 330 340 350 360 FLRDFSGSSG LFSFELTQRL TSEQVSKFMD HFQLFAMAYS WGGFESLILC NQPEEIAHIR 370 380 390 PNIKRNLTGS LIRVHIGFEN VDELIADLKA GFERIA

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21796.1.
PIR	E64049.
RefSeq	NP_438294.1.
3D structure databases
HSSP	HSSP built from PDB template 1CL2 based on UniProtKB P06721.
ModBase	Search...
Genome annotation databases
GeneID	951030.
GenomeReviews	Gene locus HI0122 in contig L42023_GR.
KEGG	hin:HI0122.
NMPDR	fig|71421.1.peg.116.
TIGR	HI0122.
Phylogenomic databases
HOGENOM	P44527.
OMA	IETTYYD.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0122-MON.
BRENDA	4.4.1.8. 109.
Family and domain databases
InterPro	IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz. IPR006233. Cys_b_lyase_bac. IPR015421. PyrdxlP-dep_Trfase_major_sub1. IPR015422. PyrdxlP-dep_Trfase_major_sub2. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit. G3DSA:3.90.1150.10. PyrdxlP-dep_Trfase_major_sub2. 1 hit.
PANTHER	PTHR11808:SF8. Cys_b_lyase_bac. 1 hit. PTHR11808. Cys_Met_Meta_PP. 1 hit.
Pfam	PF01053. Cys_Met_Meta_PP. 1 hit. [Graphical view]
PIRSF	PIRSF001434. CGS. 1 hit.
TIGRFAMs	TIGR01324. cysta_beta_ly_B. 1 hit.
PROSITE	PS00868. CYS_MET_METAB_PP. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

METC_HAEIN

Accession

Primary (citable) accession number: P44527

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 3, 2009
This is version 67 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)