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P44524 (RLMKL_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ribosomal RNA large subunit methyltransferase K/L

Including the following 2 domains:

  1. 23S rRNA m2G2445 methyltransferase
    EC=2.1.1.173
    Alternative name(s):
    rRNA (guanine-N(2)-)-methyltransferase RlmL
  2. 23S rRNA m7G2069 methyltransferase
    EC=2.1.1.264
    Alternative name(s):
    rRNA (guanine-N(7)-)-methyltransferase RlmK
Gene names
Name:rlmL
Ordered Locus Names:HI_0116
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length711 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Specifically methylates the guanine in position 2445 (m2G2445) and the guanine in position 2069 (m7G2069) of 23S rRNA By similarity. HAMAP-Rule MF_01858

Catalytic activity

S-adenosyl-L-methionine + guanine(2445) in 23S rRNA = S-adenosyl-L-homocysteine + N(2)-methylguanine(2445) in 23S rRNA. HAMAP-Rule MF_01858

S-adenosyl-L-methionine + guanine(2069) in 23S rRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(2069) in 23S rRNA. HAMAP-Rule MF_01858

Subcellular location

Cytoplasm Potential HAMAP-Rule MF_01858.

Sequence similarities

Belongs to the methyltransferase superfamily. RlmKL family.

Contains 1 THUMP domain.

Sequence caution

The sequence L42023 differs from that shown. Reason: Frameshift at position 182.

Ontologies

Keywords
   Biological processrRNA processing
   Cellular componentCytoplasm
   LigandRNA-binding
S-adenosyl-L-methionine
   Molecular functionMethyltransferase
Transferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_function23S rRNA (guanine(2445)-N(2))-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

RNA binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

rRNA (guanine-N7-)-methyltransferase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 711711Ribosomal RNA large subunit methyltransferase K/L HAMAP-Rule MF_01858
PRO_0000140474

Regions

Domain43 – 154112THUMP

Sequences

Sequence LengthMass (Da)Tools
P44524 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: BC5ABB77B60BDD03

FASTA71181,502
        10         20         30         40         50         60 
MKQLFATTSR GFEELLKVEL TELGAQEAKV VQGGVHYQAD DETLYRTLLW SRLASRILFP 

        70         80         90        100        110        120 
LIETKIYSDL DLYAAVSGFN WLAQFDERVT FFVDFNGTNQ EIRHTQFGAM RVKDGIVDYF 

       130        140        150        160        170        180 
ERQGKTRPDV DKIQPDVRIH AYLNRENLVI SLDLSGEALH LRGYREDAGQ APLRETLAAA 

       190        200        210        220        230        240 
IVMRSGWQVG SPLVDPMCGS GTLLIEAAQM EAKIAPQLYR LHWGFDFWKA HNQSAWEKVK 

       250        260        270        280        290        300 
NEAIELAEEK QREIQPHFYG FDLDHRVLKK AQKNAQNAGV SHLIKWQQAD VAALKNPRLN 

       310        320        330        340        350        360 
EVGTVICNPP YGERLGTTPA LIALYSVFGQ RLKKEFCGWN VSVFSSESTL LDCLRMRASR 

       370        380        390        400        410        420 
QFKAKNGPLD CVQKNYQVSE RKSDAITDEK ELEFNRTSEV APDFANRLQK NIKKISKWAK 

       430        440        450        460        470        480 
QQELDAYRLY DADLPEYNLA VDRYADYIVV QEYAAPKNID ENKARQRLLD AVTATLQVTG 

       490        500        510        520        530        540 
VETNKLILKV RQKQKGTNQY EKLANKGEYF YVNEYGTQLW VNLTDYLDTG LFLDHRLTRK 

       550        560        570        580        590        600 
MIGELAKGKD FLNLFAYTGS ATVHAALGGA KSTTTVDMSN TYLNWAEQNL ILNDIEGKQH 

       610        620        630        640        650        660 
KLIQADCLQW LEKCDRQFDL IFADPPTFSN SKRMEESWDV QRDHVKLMRN LKRVLSNNGT 

       670        680        690        700        710 
IVFSNNKRGF KMNLVALEEL GLSAIEISHK TLPLDFERNK QIHNCWMIQH I 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. No translation available.

3D structure databases

ProteinModelPortalP44524.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

OMANKRGFKM.

Family and domain databases

HAMAPMF_01858. 23SrRNA_methyltr_KL.
InterProIPR017244. 23SrRNA_methyltr_KL.
IPR002052. DNA_methylase_N6_adenine_CS.
IPR000241. RNA_methylase_dom.
IPR019614. SAM-dep_methyl-trfase.
IPR004114. THUMP.
[Graphical view]
PfamPF10672. Methyltrans_SAM. 1 hit.
PF02926. THUMP. 1 hit.
PF01170. UPF0020. 1 hit.
[Graphical view]
PIRSFPIRSF037618. RNA_Mtase_bacteria_prd. 1 hit.
SMARTSM00981. THUMP. 1 hit.
[Graphical view]
PROSITEPS51165. THUMP. 1 hit.
PS01261. UPF0020. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameRLMKL_HAEIN
AccessionPrimary (citable) accession number: P44524
Secondary accession number(s): P43945
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1997
Last modified: April 16, 2014
This is version 89 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names