Bifunctional aspartokinase/homoserine dehydrogenase

Contribute

Send feedback

Reviewed, UniProtKB/Swiss-Prot P44505 (AKH_HAEIN)

Last modified July 22, 2008. Version 60. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data |

Customize display

text xml rdf/xml gff fasta

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Hide | Top

Names and origin

Protein names

Recommended name:
    Bifunctional aspartokinase/homoserine dehydrogenase
      Short name=AK-HD
Including the following 2 domains:
    1- Recommended name:
            Aspartokinase
              EC=2.7.2.4
    2- Recommended name:
            Homoserine dehydrogenase
              EC=1.1.1.3

Gene names

Name:	thrA
Ordered Locus Names:	HI0089

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Hide | Top

Protein attributes

Sequence length	815 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

Hide | Top

General annotation (Comments)

Catalytic activity	L-homoserine + NAD(P)(+) = L-aspartate 4-semialdehyde + NAD(P)H. ATP + L-aspartate = ADP + 4-phospho-L-aspartate.
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; tetrahydrodipicolinate from L-aspartate: step 1/4. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 1/3. Amino-acid biosynthesis; L-methionine biosynthesis via de novo pathway; L-homoserine from L-aspartate: step 3/3. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 1/5. Amino-acid biosynthesis; L-threonine biosynthesis; L-threonine from L-aspartate: step 3/5.
Subunit structure	Homotetramer By similarity.
Sequence similarities	In the N-terminal section; belongs to the aspartokinase family. In the C-terminal section; belongs to the homoserine dehydrogenase family. Contains 2 ACT domains.

Hide | Top

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Threonine biosynthesis
Domain	Repeat
Ligand	NADP
Molecular function	Kinase Oxidoreductase Transferase
Technical term	Allosteric enzyme Complete proteome Multifunctional enzyme
Gene Ontology (GO)
None. [Check GOA]

Hide | Top

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Molecule processing

Chain

1 – 815

815

Bifunctional aspartokinase/homoserine dehydrogenase

Regions

Domain

316 – 390

ACT 1

Domain

397 – 471

ACT 2

Nucleotide binding

471 – 478

NADP Potential

Region

1 – 249

249

Aspartokinase

Region

250 – 470

221

Interface

Region

471 – 815

345

Homoserine dehydrogenase

Hide | Top

Sequences

Sequence

Length

Mass (Da)

Tools

P44505-1 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 7FD5512FCC4BE7F7
Show »

FASTA

815

88,221

        10         20         30         40         50         60 
MRVLKFGGTS LANPERFSQA AKLIEQAHLE EQAAGVLSAP AKITNHLVAL SEKAALNQST 

        70         80         90        100        110        120 
DTHFNEAIEI FYNIINGLHT ENNQFDLNGT KALIDAEFVQ IKGLLEEIRQ AGKVEDAVKA 

       130        140        150        160        170        180 
TIDCRGEKLS IAMMKAWFEA RGYSVHIVDP VKQLLAKGGY LESSVEIEES TKRVDAANIA 

       190        200        210        220        230        240 
KDKVVLMAGF TAGNEKGELV LLGRNGSDYS AACLAACLGA SVCEIWTDVD GVYTCDPRLV 

       250        260        270        280        290        300 
PDARLLPTLS YREAMELSYF GAKVIHPRTI GPLLPQNIPC VIKNTGNPSA PGSIIDGNVK 

       310        320        330        340        350        360 
SESLQVKGIT NLDNLAMFNV SGPGMQGMVG MASRVFSAMS GAGISVILIT QSSSEYSISF 

       370        380        390        400        410        420 
CVPVKSAEVA KTVLETEFAN ELNEHQLEPI EVIKDLSIIS VVGDGMKQAK GIAARFFSAL 

       430        440        450        460        470        480 
AQANISIVAI AQGSSERSIS AVVPQNKAIE AVKATHQALF NNKKVVDMFL VGVGGVGGEL 

       490        500        510        520        530        540 
IEQVKRQKEY LAKKNVEIRV CAIANSNRML LDENGLNLED WKNDLENATQ PSDFDVLLSF 

       550        560        570        580        590        600 
IKLHHVVNPV FVDCTSAESV AGLYARALKE GFHVVTPNKK ANTRELVYYN ELRQNAQASQ 

       610        620        630        640        650        660 
HKFLYETNVG AGLPVIENLQ NLLAAGDELE YFEGILSGSL SFIFGKLEEG LSLSEVTALA 

       670        680        690        700        710        720 
REKGFTEPDP RDDLSGQDVA RKLLILAREA GIELELSDVE VEGVLPKGFS DGKSADEFMA 

       730        740        750        760        770        780 
MLPQLDEEFK TRVATAKAEG KVLRYVGKIS EGKCKVSIVA VDLNNPLYKV KDGENALAFY 

       790        800        810 
TRYYQPIPLL LRGYGAGNAV TAAGIFADIL RTLQH

« Hide

Hide | Top

Cross-references

Sequence databases
	L42023 Genomic DNA. Translation: AAC21767.1.
PIR	A64048.
RefSeq	NP_438262.1.
3D structure databases
HSSP	HSSP built from PDB template 1EBF based on UniProtKB P31116.
ModBase	Search...
Genome annotation databases
GeneID	950991.
GenomeReviews	Gene locus HI0089 in contig L42023_GR.
KEGG	hin:HI0089.
NMPDR	fig\|71421.1.peg.88.
TIGR	HI0089.
Phylogenomic databases
HOGENOM	P44505.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0089-MON.
Family and domain databases
InterPro	IPR002912. ACT_bd. IPR001048. Asp/Glu/Uridylate_kinase. IPR005106. Asp/hSer_DHase_NAD-bd. IPR001341. Asp_kin_reg. IPR011147. bifunc_aspartokin/hSer_DHase. IPR001342. hSer_DHase_cat. IPR016040. NAD(P)-bd. [Graphical view]
Gene3D	G3DSA:3.40.1160.10. Aa_kinase. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
Pfam	PF00696. AA_kinase. 1 hit. PF01842. ACT. 2 hits. PF00742. Homoserine_dh. 1 hit. PF03447. NAD_binding_3. 1 hit. [Graphical view]
PIRSF	PIRSF000727. ThrA. 1 hit.
TIGRFAMs	TIGR00657. asp_kinases. 1 hit.
PROSITE	PS00324. ASPARTOKINASE. 1 hit. PS01042. HOMOSER_DHGENASE. 1 hit. [Graphical view]
ProDom	P44505. [Graphical view] [Entries sharing at least one domain]
BLOCKS	Search...
Other Resources
ProtoNet	Search...

Hide | Top

Entry information

Entry name

AKH_HAEIN

Accession

Primary (citable) accession number: P44505

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	July 22, 2008
This is version 60 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Hide | Top

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Keywords

Gene Ontology (GO)

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Genome annotation databases

Phylogenomic databases

Enzyme and pathway databases

Family and domain databases

Other Resources

Entry information

Relevant documents