Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P44502 (METB_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Cystathionine gamma-synthase

Short name=CGS
EC=2.5.1.48
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene names
Name:metB
Ordered Locus Names:HI_0086
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) [Reference proteome] [HAMAP]
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus

Protein attributes

Sequence length369 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia By similarity.

Catalytic activity

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactor

Binds 1 pyridoxal phosphate per subunit By similarity.

Subunit structure

Homotetramer By similarity.

Subcellular location

Cytoplasm By similarity.

Sequence similarities

Belongs to the trans-sulfuration enzymes family.

Sequence caution

The sequence AAC21764.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processAmino-acid biosynthesis
Methionine biosynthesis
   Cellular componentCytoplasm
   LigandPyridoxal phosphate
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processmethionine biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functioncystathionine gamma-synthase activity

Inferred from electronic annotation. Source: UniProtKB-EC

pyridoxal phosphate binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 369369Cystathionine gamma-synthase
PRO_0000114757

Amino acid modifications

Modified residue2001N6-(pyridoxal phosphate)lysine By similarity

Sequences

Sequence LengthMass (Da)Tools
P44502 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: D76A8A03FEAF7A14

FASTA36940,371
        10         20         30         40         50         60 
MTQQYAIDTL LAQAGNRSDE RTGAVSAPIF LSTAYGHCGI GESTGFDYTR TKNPTRTVLE 

        70         80         90        100        110        120 
ETIAKLENGD RGFAFSSGMA AIQVLMTLFT APDEWIVSSD VYGGTYRLLD FSYKNNNSVK 

       130        140        150        160        170        180 
PVYVNTASAS AIEAAINPNT KAIFIETPSN PLMEECDVVE IAKLAKKHNL MLIVDNTFLT 

       190        200        210        220        230        240 
PVLSRPLDLG ADVVIHSGTK YIAGHNDALV GLIVAKGQEL CDRIAYIQNG AGAVLSPFDS 

       250        260        270        280        290        300 
WLTIRGMKTL SLRMKRHQEN AQAIAEFLKA QPQVESVLYP NKGGMLSFRL QDEAWVNTFL 

       310        320        330        340        350        360 
KSIKLITFAE SLGGTESFIT YPATQTHMDI PESERVARGI TNTLLRFSVG IEDVEDIKAD 


LLQAFANLK 

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L42023 Genomic DNA. Translation: AAC21764.1. Different initiation.
RefSeqNP_438259.1. NC_000907.1.

3D structure databases

ProteinModelPortalP44502.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING71421.HI0086.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC21764; AAC21764; HI_0086.
GeneID950982.
KEGGhin:HI0086.
PATRIC20188627. VBIHaeInf48452_0087.

Phylogenomic databases

eggNOGCOG0626.
KOK01739.
OMATPIFLST.
OrthoDBEOG67DPN3.

Family and domain databases

Gene3D3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERPTHR11808. PTHR11808. 1 hit.
PfamPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFPIRSF001434. CGS. 1 hit.
SUPFAMSSF53383. SSF53383. 1 hit.
PROSITEPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMETB_HAEIN
AccessionPrimary (citable) accession number: P44502
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: May 14, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names