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Protein

Cystathionine gamma-synthase

Gene

metB

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of L-cystathionine from O-succinyl-L-homoserine (OSHS) and L-cysteine, via a gamma-replacement reaction. In the absence of thiol, catalyzes gamma-elimination to form 2-oxobutanoate, succinate and ammonia (By similarity).By similarity

Catalytic activityi

O(4)-succinyl-L-homoserine + L-cysteine = L-cystathionine + succinate.

Cofactori

pyridoxal 5'-phosphateBy similarityNote: Binds 1 pyridoxal phosphate per subunit.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Methionine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-synthase (EC:2.5.1.48)
Short name:
CGS
Alternative name(s):
O-succinylhomoserine (thiol)-lyase
Gene namesi
Name:metB
Ordered Locus Names:HI_0086
OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifieri71421 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
Proteomesi
  • UP000000579 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001147571 – 369Cystathionine gamma-synthaseAdd BLAST369

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei200N6-(pyridoxal phosphate)lysineBy similarity1

Interactioni

Subunit structurei

Homotetramer.By similarity

Protein-protein interaction databases

STRINGi71421.HI0086.

Structurei

3D structure databases

ProteinModelPortaliP44502.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
KOiK01739.
OMAiSPIDCYL.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P44502-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTQQYAIDTL LAQAGNRSDE RTGAVSAPIF LSTAYGHCGI GESTGFDYTR
60 70 80 90 100
TKNPTRTVLE ETIAKLENGD RGFAFSSGMA AIQVLMTLFT APDEWIVSSD
110 120 130 140 150
VYGGTYRLLD FSYKNNNSVK PVYVNTASAS AIEAAINPNT KAIFIETPSN
160 170 180 190 200
PLMEECDVVE IAKLAKKHNL MLIVDNTFLT PVLSRPLDLG ADVVIHSGTK
210 220 230 240 250
YIAGHNDALV GLIVAKGQEL CDRIAYIQNG AGAVLSPFDS WLTIRGMKTL
260 270 280 290 300
SLRMKRHQEN AQAIAEFLKA QPQVESVLYP NKGGMLSFRL QDEAWVNTFL
310 320 330 340 350
KSIKLITFAE SLGGTESFIT YPATQTHMDI PESERVARGI TNTLLRFSVG
360
IEDVEDIKAD LLQAFANLK
Length:369
Mass (Da):40,371
Last modified:November 1, 1995 - v1
Checksum:iD76A8A03FEAF7A14
GO

Sequence cautioni

The sequence AAC21764 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21764.1. Different initiation.
RefSeqiNP_438259.1. NC_000907.1.
WP_005693832.1. NC_000907.1.

Genome annotation databases

EnsemblBacteriaiAAC21764; AAC21764; HI_0086.
GeneIDi950982.
KEGGihin:HI0086.
PATRICi20188627. VBIHaeInf48452_0087.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42023 Genomic DNA. Translation: AAC21764.1. Different initiation.
RefSeqiNP_438259.1. NC_000907.1.
WP_005693832.1. NC_000907.1.

3D structure databases

ProteinModelPortaliP44502.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi71421.HI0086.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC21764; AAC21764; HI_0086.
GeneIDi950982.
KEGGihin:HI0086.
PATRICi20188627. VBIHaeInf48452_0087.

Phylogenomic databases

eggNOGiENOG4105C28. Bacteria.
COG0626. LUCA.
KOiK01739.
OMAiSPIDCYL.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiMETB_HAEIN
AccessioniPrimary (citable) accession number: P44502
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: November 30, 2016
This is version 111 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Haemophilus influenzae
    Haemophilus influenzae (strain Rd): entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.