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P44497

- NADK_HAEIN

UniProt

P44497 - NADK_HAEIN

Protein

NAD kinase

Gene

nadK

Organism
Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 96 (01 Oct 2014)
      Sequence version 3 (04 May 2001)
      Previous versions | rss
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    Functioni

    Involved in the regulation of the intracellular balance of NAD and NADP, and is a key enzyme in the biosynthesis of NADP. Catalyzes specifically the phosphorylation on 2'-hydroxyl of the adenosine moiety of NAD to yield NADP.UniRule annotation

    Catalytic activityi

    ATP + NAD+ = ADP + NADP+.UniRule annotation

    Cofactori

    Divalent metal ions.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei76 – 761Proton acceptorUniRule annotation
    Binding sitei162 – 1621NADUniRule annotation
    Binding sitei179 – 1791NADUniRule annotation
    Binding sitei181 – 1811NADUniRule annotation
    Binding sitei252 – 2521NADUniRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi76 – 772NADUniRule annotation
    Nucleotide bindingi151 – 1522NADUniRule annotation
    Nucleotide bindingi192 – 1976NADUniRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: UniProtKB-HAMAP
    3. NAD+ kinase activity Source: UniProtKB-HAMAP

    GO - Biological processi

    1. NAD metabolic process Source: InterPro
    2. NADP biosynthetic process Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Kinase, Transferase

    Keywords - Ligandi

    ATP-binding, NAD, NADP, Nucleotide-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    NAD kinaseUniRule annotation (EC:2.7.1.23UniRule annotation)
    Alternative name(s):
    ATP-dependent NAD kinaseUniRule annotation
    Gene namesi
    Name:nadKUniRule annotation
    Ordered Locus Names:HI_0072
    OrganismiHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
    Taxonomic identifieri71421 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPasteurellalesPasteurellaceaeHaemophilus
    ProteomesiUP000000579: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 285285NAD kinasePRO_0000120622Add
    BLAST

    Interactioni

    Protein-protein interaction databases

    STRINGi71421.HI0072.

    Structurei

    3D structure databases

    ProteinModelPortaliP44497.
    SMRiP44497. Positions 7-278.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the NAD kinase family.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0061.
    KOiK00858.
    OMAiDGHINID.
    OrthoDBiEOG6PZXDR.

    Family and domain databases

    Gene3Di2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPiMF_00361. NAD_kinase.
    InterProiIPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view]
    PANTHERiPTHR20275. PTHR20275. 1 hit.
    PfamiPF01513. NAD_kinase. 1 hit.
    [Graphical view]
    SUPFAMiSSF111331. SSF111331. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P44497-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNHLYRSFKT IALVGKPRND INLQMHKNLF HWLMERGYQV LVEKEVAITL    50
    ELPFEHLATL EEIGHRAQLA IVIGGDGNML GRARVLAKYD IPLIGINRGN 100
    LGFLTDIDPK NAYSQLEACL ERGEFFVEER FLLEAKIERA SEIVSTSNAV 150
    NEAVIHPAKI AHMIDFHVYI NDKFAFSQRS DGLIVSTPTG STAYSLSAGG 200
    PILTPNLNAI ALVPMFPHTL TSRPLVVDGD SKISIRFAEH NTSQLEVGCD 250
    SQITLPFTPD DVVHIQKSEH KLRLLHLKII IITMC 285
    Length:285
    Mass (Da):31,828
    Last modified:May 4, 2001 - v3
    Checksum:iD49647085A2025AB
    GO

    Sequence cautioni

    The sequence AAC21749.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21749.1. Different initiation.
    PIRiI64141.
    RefSeqiNP_438244.1. NC_000907.1.

    Genome annotation databases

    EnsemblBacteriaiAAC21749; AAC21749; HI_0072.
    GeneIDi950969.
    KEGGihin:HI0072.
    PATRICi20188597. VBIHaeInf48452_0072.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L42023 Genomic DNA. Translation: AAC21749.1 . Different initiation.
    PIRi I64141.
    RefSeqi NP_438244.1. NC_000907.1.

    3D structure databases

    ProteinModelPortali P44497.
    SMRi P44497. Positions 7-278.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 71421.HI0072.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC21749 ; AAC21749 ; HI_0072 .
    GeneIDi 950969.
    KEGGi hin:HI0072.
    PATRICi 20188597. VBIHaeInf48452_0072.

    Phylogenomic databases

    eggNOGi COG0061.
    KOi K00858.
    OMAi DGHINID.
    OrthoDBi EOG6PZXDR.

    Family and domain databases

    Gene3Di 2.60.200.30. 1 hit.
    3.40.50.10330. 1 hit.
    HAMAPi MF_00361. NAD_kinase.
    InterProi IPR017438. ATP-NAD_kinase_dom_1.
    IPR016064. ATP-NAD_kinase_PpnK-typ.
    IPR017437. ATP-NAD_kinase_PpnK-typ_all-b.
    IPR002504. PolyP/ATP_NADK.
    [Graphical view ]
    PANTHERi PTHR20275. PTHR20275. 1 hit.
    Pfami PF01513. NAD_kinase. 1 hit.
    [Graphical view ]
    SUPFAMi SSF111331. SSF111331. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.
    2. White O., Clayton R.A., Kerlavage A.R., Fleischmann R.D.
      Submitted (SEP-1996) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Two-dimensional map of the proteome of Haemophilus influenzae."
      Langen H., Takacs B., Evers S., Berndt P., Lahm H.W., Wipf B., Gray C., Fountoulakis M.
      Electrophoresis 21:411-429(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

    Entry informationi

    Entry nameiNADK_HAEIN
    AccessioniPrimary (citable) accession number: P44497
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 1, 1995
    Last sequence update: May 4, 2001
    Last modified: October 1, 2014
    This is version 96 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Haemophilus influenzae
      Haemophilus influenzae (strain Rd): entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3