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P44495 (MIAA_HAEIN) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
tRNA dimethylallyltransferase
EC=2.5.1.75
Alternative name(s):
Dimethylallyl diphosphate:tRNA dimethylallyltransferase
Short name=DMAPP:tRNA dimethylallyltransferase
Short name=DMATase
Isopentenyl-diphosphate:tRNA isopentenyltransferase
Short name=IPP transferase
Short name=IPPT
Short name=IPTase
Gene names
Name:miaA
Synonyms:trpX
Ordered Locus Names:HI_0068
OrganismHaemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd)
Taxonomic identifier71421 [NCBI]
Taxonomic lineageBacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length311 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity. HAMAP MF_00185

Catalytic activity

Dimethylallyl diphosphate + tRNA = diphosphate + tRNA containing 6-dimethylallyladenosine. HAMAP MF_00185

Cofactor

Magnesium By similarity. HAMAP MF_00185

Subunit structure

Monomer By similarity. HAMAP MF_00185

Sequence similarities

Belongs to the IPP transferase family.

Ontologies

Keywords
   Biological processtRNA processing
   LigandATP-binding
Magnesium
Nucleotide-binding
   Molecular functionTransferase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological processtRNA processing

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

tRNA dimethylallyltransferase activity

Inferred from electronic annotation. Source: EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 311311tRNA dimethylallyltransferase HAMAP MF_00185
PRO_0000163924

Regions

Nucleotide binding10 – 178ATP Potential
Region12 – 176Substrate binding By similarity
Region35 – 384Interaction with substrate tRNA By similarity
Region159 – 1635Interaction with substrate tRNA By similarity
Region240 – 2456Interaction with substrate tRNA By similarity

Sites

Site1011Interaction with substrate tRNA By similarity
Site1231Interaction with substrate tRNA By similarity

Sequences

Sequence LengthMass (Da)Tools
P44495 [UniParc].

Last modified November 1, 1995. Version 1.
Checksum: 4B5E3EEFE28DC637

FASTA31135,204
        10         20         30         40         50         60 
MKPTAIFLMG PTASGKTDLA IQLRSQLPVE VISVDSALIY KGMDIGTAKP SKEELALAPH 

        70         80         90        100        110        120 
RLIDILDPSE SYSAMNFRDD ALREMADITA QGKIPLLVGG TMLYYKALIE GLSPLPSADE 

       130        140        150        160        170        180 
NIRAELEQKA AQQGWAALHT ELAKIDPISA ARINPSDSQR INRALEVFYI TGKSLTELTE 

       190        200        210        220        230        240 
EKGEALPYDF VQFAIAPQDR HVLHERIEQR FHKMIELGFQ AEVEKLYARG DLNINLPSIR 

       250        260        270        280        290        300 
CVGYRQMWEY LQGDYAYEEM IFRGICATRQ LAKRQLTWLR GWKTPIQWLD SLQPQQAKET 

       310 
VLRHLDSYQK G

« Hide

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		L42023 Genomic DNA. Translation: AAC21746.1.
PIRF64046.
RefSeqNP_438241.1. NC_000907.1.

3D structure databases

ProteinModelPortalP44495.
SMRP44495. Positions 3-305.
ModBaseSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID950968.
GenomeReviewsGene locus HI_0068 in contig L42023_GR.
KEGGhin:HI0068.
NMPDRfig|71421.1.peg.67.
PATRIC20188591. VBIHaeInf48452_0069.
TIGRHI_0068.

Phylogenomic databases

HOGENOMHBG559459.
OMAHTRNYAK.
PhylomeDBP44495.
ProtClustDBPRK00091.

Enzyme and pathway databases

BioCycHINF71421:HI_0068-MONOMER.

Family and domain databases

HAMAPMF_00185. IPP_trans.
[Tree]
InterProIPR002627. IPPT.
IPR018022. tRNA_delta_PyrP_Trfase.
[Graphical view]
KOK00791.
PANTHERPTHR11088. IPPT. 1 hit.
PfamPF01715. IPPT. 1 hit.
[Graphical view]
TIGRFAMsTIGR00174. MiaA. 1 hit.
ProtoNetSearch...

Entry information

Entry nameMIAA_HAEIN
AccessionPrimary (citable) accession number: P44495
Entry history
Integrated into UniProtKB/Swiss-Prot: November 1, 1995
Last sequence update: November 1, 1995
Last modified: January 25, 2012
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Haemophilus influenzae

Haemophilus influenzae (strain Rd): entries and gene names

SIMILARITY comments

Index of protein domains and families

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