Reviewed, UniProtKB/Swiss-Prot P44495 (MIAA_HAEIN)

Names and origin

Protein names

Recommended name:
    tRNA Delta(2)-isopentenylpyrophosphate transferase
      Short name=IPP transferase
    EC=2.5.1.8
Alternative name(s):
    Isopentenyl-diphosphate:tRNA isopentenyltransferase
      Short name=IPTase
      Short name=IPPT
    Dimethylallyl diphosphate:tRNA dimethylallyltransferase
      Short name=DMAPP:tRNA dimethylallyltransferase
      Short name=DMATase

Gene names

Name:	miaA
Synonyms:	trpX
Ordered Locus Names:	HI0068

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	311 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i6A) By similarity.
Catalytic activity	Isopentenyl diphosphate + tRNA = diphosphate + tRNA containing 6-isopentenyladenosine. HAMAP MF_00185
Cofactor	Magnesium By similarity.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the IPP transferase family.

Ontologies

Keywords
Biological process	tRNA processing
Ligand	ATP-binding Magnesium Nucleotide-binding
Molecular function	Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	tRNA processing Inferred from electronic annotation. Source: HAMAP
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW nucleotidyltransferase activity Inferred from electronic annotation. Source: UniProtKB-KW tRNA isopentenyltransferase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 311	311	tRNA Delta(2)-isopentenylpyrophosphate transferase HAMAP MF_00185		PRO_0000163924
Regions
	Nucleotide binding	10 – 17	8	ATP Potential
	Region	12 – 17	6	Substrate binding By similarity
	Region	35 – 38	4	Interaction with substrate tRNA By similarity
	Region	159 – 163	5	Interaction with substrate tRNA By similarity
	Region	240 – 245	6	Interaction with substrate tRNA By similarity
Sites
	Site	101	1	Interaction with substrate tRNA By similarity
	Site	123	1	Interaction with substrate tRNA By similarity

Sequences

	Sequence		Length	Mass (Da)
	P44495-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 4B5E3EEFE28DC637	FASTA	311	35,204
	10 20 30 40 50 60 MKPTAIFLMG PTASGKTDLA IQLRSQLPVE VISVDSALIY KGMDIGTAKP SKEELALAPH 70 80 90 100 110 120 RLIDILDPSE SYSAMNFRDD ALREMADITA QGKIPLLVGG TMLYYKALIE GLSPLPSADE 130 140 150 160 170 180 NIRAELEQKA AQQGWAALHT ELAKIDPISA ARINPSDSQR INRALEVFYI TGKSLTELTE 190 200 210 220 230 240 EKGEALPYDF VQFAIAPQDR HVLHERIEQR FHKMIELGFQ AEVEKLYARG DLNINLPSIR 250 260 270 280 290 300 CVGYRQMWEY LQGDYAYEEM IFRGICATRQ LAKRQLTWLR GWKTPIQWLD SLQPQQAKET 310 VLRHLDSYQK G

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBLGenBankDDBJ	L42023 Genomic DNA. Translation: AAC21746.1.
PIR	F64046.
RefSeq	NP_438241.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	950968.
GenomeReviews	Gene locus HI0068 in contig L42023_GR.
KEGG	hin:HI0068.
NMPDR	fig|71421.1.peg.67.
TIGR	HI0068.
Phylogenomic databases
HOGENOM	P44495.
OMA	VNADSMQ
Enzyme and pathway databases
BioCyc	HINF71421:HI_0068-MON.
BRENDA	2.5.1.8. 109.
Family and domain databases
HAMAP	MF_00185. [Tree]
InterPro	IPR002627. IPPT. IPR018022. tRNA_delta_PyrP_Trfase. [Graphical view]
PANTHER	PTHR11088. IPPT. 1 hit.
Pfam	PF01715. IPPT. 1 hit. [Graphical view]
TIGRFAMs	TIGR00174. miaA. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MIAA_HAEIN

Accession

Primary (citable) accession number: P44495

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	November 24, 2009
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)