Reviewed, UniProtKB/Swiss-Prot P44493 (AMIB_HAEIN)

Names and origin

Protein names

Recommended name:
    Probable N-acetylmuramoyl-L-alanine amidase amiB
    EC=3.5.1.28

Gene names

Name:	amiB
Ordered Locus Names:	HI0066

Organism

Haemophilus influenzae [Complete proteome] [HAMAP]

Taxonomic identifier

727 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Pasteurellales › Pasteurellaceae › Haemophilus

Protein attributes

Sequence length	432 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Cell-wall hydrolase probably involved in cell-wall hydrolysis, septation or recycling By similarity.
Catalytic activity	Hydrolyzes the link between N-acetylmuramoyl residues and L-amino acid residues in certain cell-wall glycopeptides.
Subcellular location	Secreted Potential.
Domain	LysM repeats are thought to be involved in peptidoglycan binding.
Sequence similarities	Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family. Contains 2 LysM repeats.

Ontologies

Keywords
Biological process	Cell wall biogenesis/degradation
Cellular component	Secreted
Domain	Repeat Signal
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell wall macromolecule catabolic process Inferred from electronic annotation. Source: InterPro cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	N-acetylmuramoyl-L-alanine amidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	Potential
	Chain	21 – 432	412	Probable N-acetylmuramoyl-L-alanine amidase amiB		PRO_0000006464
Regions
	Repeat	294 – 336	43	LysM 1
	Repeat	387 – 430	44	LysM 2

Sequences

	Sequence		Length	Mass (Da)
	P44493-1 [UniParc]. Last modified November 1, 1995. Version 1. Checksum: 008D918A0E1EE81D	FASTA	432	49,109
	10 20 30 40 50 60 MKTKILFFLF FSTFSFSIFA APITIAIDPG HGGKDPGAIS RNLGIYEKNV TLSIAKELKA 70 80 90 100 110 120 LLDKDPHFRG VLTRKSDYYI SVPERSEIAR KFKANYLISI HADSSKSPDR RGASVWVLSN 130 140 150 160 170 180 RRANDEMGQW LEDDEKRSEL LGGAGKVLSH NNDKYLDQTV LDLQFGHSQR TGYVLGEHIL 190 200 210 220 230 240 HHFAKVTTLS RSTPQHASLG VLRSPDIPSV LVETGFLSNS EEEKKLNSQT YRRRIAYMIY 250 260 270 280 290 300 EGLVAFHSGK TNTLVKDNLV QNIKQNDIKK SGKNNRTSEQ NINEDNIKDS GIRHIVKKGE 310 320 330 340 350 360 SLGSLSNKYH VKVSDIIKLN QLKRKTLWLN ESIKIPDNVE IKNKSLTIKE NDFHKKQNSL 370 380 390 400 410 420 VNNTNKDLKK EKNTQTNNQK NIIPLYHKVT KNQTLYAISR EYNIPVNILL SLNPHLKNGK 430 VITGQKIKLR EK

References

[1]

"Whole-genome random sequencing and assembly of Haemophilus influenzae Rd." Fleischmann R.D., Adams M.D., White O., Clayton R.A., Kirkness E.F., Kerlavage A.R., Bult C.J., Tomb J.-F., Dougherty B.A., Merrick J.M., McKenney K., Sutton G.G., FitzHugh W., Fields C.A., Gocayne J.D., Scott J.D., Shirley R., Liu L.-I. , Glodek A., Kelley J.M., Weidman J.F., Phillips C.A., Spriggs T., Hedblom E., Cotton M.D., Utterback T.R., Hanna M.C., Nguyen D.T., Saudek D.M., Brandon R.C., Fine L.D., Fritchman J.L., Fuhrmann J.L., Geoghagen N.S.M., Gnehm C.L., McDonald L.A., Small K.V., Fraser C.M., Smith H.O., Venter J.C. Science 269:496-512(1995) [PubMed: 7542800] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 51907 / DSM 11121 / KW20 / Rd.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L42023 Genomic DNA. Translation: AAC21744.1.
PIR	D64046.
RefSeq	NP_438239.1.
3D structure databases
SMR	P44493. Positions 24-247.
ModBase	Search...
Genome annotation databases
GeneID	950965.
GenomeReviews	Gene locus HI0066 in contig L42023_GR.
KEGG	hin:HI0066.
NMPDR	fig|71421.1.peg.65.
TIGR	HI0066.
Phylogenomic databases
HOGENOM	HBG656726.
OMA	QKIKLRE.
Enzyme and pathway databases
BioCyc	HINF71421:HI_0066-MONOMER.
BRENDA	3.5.1.28. 109.
Family and domain databases
InterPro	IPR002508. CW_Hdrlase/autolysin_cat. IPR018392. Peptidoglycan-bd_lysin. IPR002482. Peptidoglycan-bd_Lysin_sg. [Graphical view]
Gene3D	G3DSA:3.40.630.40. Cell_wall_OHase/autolysin_cat. 1 hit.
Pfam	PF01520. Amidase_3. 1 hit. PF01476. LysM. 2 hits. [Graphical view]
SMART	SM00646. Ami_3. 1 hit. SM00257. LysM. 2 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AMIB_HAEIN

Accession

Primary (citable) accession number: P44493

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 1, 1995
Last sequence update:	November 1, 1995
Last modified:	January 19, 2010
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)